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Use Of Shoot-Root Grafts In Case Studies Of Site Of O-Hydroxycinnamic Acid Synthesis In Melilotus Alba, Herman J. Gorz, Francis A. Haskins
Use Of Shoot-Root Grafts In Case Studies Of Site Of O-Hydroxycinnamic Acid Synthesis In Melilotus Alba, Herman J. Gorz, Francis A. Haskins
Department of Agronomy and Horticulture: Faculty Publications
The content of o-hydroxycinnamic acid was measured in shoot-root grafts involving four combinations of Melilotus alba plants of the CuCuBB and cucuBB genotypes. Six portions from each grafted plant were assayed. Results provide evidence that o-hydroxycinnamic acid synthesis occurs in the above-ground parts of sweetclover plants, with limited translocation to the roots. No support was obtained for the view that the root plays an important role in o-hydroxycinnamic acid synthesis.
Genetic And Biochemical Aspects Of O-Hydroxycinnamic Acid Synthesis In Melilotus Alba, Herman J. Gorz, Francis A. Haskins, A. Kleinhofs
Genetic And Biochemical Aspects Of O-Hydroxycinnamic Acid Synthesis In Melilotus Alba, Herman J. Gorz, Francis A. Haskins, A. Kleinhofs
Department of Agronomy and Horticulture: Faculty Publications
In sweetclover (Melilotus alba Desr.) the cis- and trans-isomers of o-hydroxycinnamic acid occur primarily as the respective, β-D-glucosides. Available evidence indicates that these glucosides are formed via the following pathway: phenylalanine (formed from shikimic acid) → trans-cinnamic acid → o-coumaric acid (trans-o-hydroxycinnamic acid) → o-coumaryl glucoside (trans-β-D-glucosyl-o-hydroxycinnamic acid) → coumarinyl glucoside (cis-β-D-glucosyl-o-hydroxycinnamic acid). In tissues that are disrupted, coumarinyl glucoside is rapidly hydrolyzed by the action of endogenous β-glucosidase to yield coumarinic acid, which lactonizes spontaneously to form coumarin. The cu …