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Resistance Gene Analog Polymorphism (Rgap) Markers Co-Localize With Disease Resistance Genes And Qtl In Common Bean, Nedim Mutlu, Phillip Miklas, Dermot Coyne
Resistance Gene Analog Polymorphism (Rgap) Markers Co-Localize With Disease Resistance Genes And Qtl In Common Bean, Nedim Mutlu, Phillip Miklas, Dermot Coyne
Department of Agronomy and Horticulture: Faculty Publications
Resistance (R) genes containing nucleotide-binding site (NBS)-leucine rich repeats (LRR) are the most prevalent types of R gene in plants. The objective of this study was to develop PCR-based R-gene analog polymorphism (RGAP) markers for common bean (Phaseolus vulgaris L). Twenty degenerate primers were designed from the conserved kinase-1a (GVGKTT) and hydrophobic domains (GLPLAL) of known NBSLRR type R-genes and from EST databases. Sixty-six of the 100 primer combinations tested yielded polymorphism. Thirty-two RGAP markers were mapped in the BAT 93/Jalo EEP558 core mapping population for common bean. The markers mapped to 10 of 11 linkage groups with a …