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Assessing Multiple-Herbicide Resistance In A 2,4-D Resistant Waterhemp (Amaranthus Tuberculatus) Biotype From Nebraska – Student Research, Roberto Crespo

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

A waterhemp biotype was recently reported resistant to 2,4-D in Nebraska. In addition to the repeated use of 2,4-D, atrazine and imazathapyr were reported to be frequently used to control broadleaf weeds. Greenhouse experiments were conducted to confirm 2,4-D resistance and test for resistance to growth regulator herbicides and other herbicide modes-of-action (MoA). A single dose-response experiment showed reduced sensitivity in all three waterhemp biotypes to atrazine, imazethapyr and lactofen; therefore, they were generally considered resistant to those three herbicide MoA. None of the biotypes contained the Ser264 target-site mutation. Since the ametryn dose-response experiment resulted in all susceptible biotypes, …