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Molecular Basis Of The Ribulose-1,5-Bisphosphate Carboxylase/Oxygenase Activase Mutation In Arabidopsis Thaliana Is A Guanine-To-Adenine Transition At The 5'-Splice Junction Of Intron 3, Beverly M. Orozco, C Robertson Mcclung, Jeffrey M. Werneke, William L. Ogren
Molecular Basis Of The Ribulose-1,5-Bisphosphate Carboxylase/Oxygenase Activase Mutation In Arabidopsis Thaliana Is A Guanine-To-Adenine Transition At The 5'-Splice Junction Of Intron 3, Beverly M. Orozco, C Robertson Mcclung, Jeffrey M. Werneke, William L. Ogren
Dartmouth Scholarship
Analysis of the ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) activase gene and gene products from Arabidopsis thaliana wild-type plants and the Rubisco activase-deficient mutant strain showed that the rca mutation caused GT to be changed to AT at the 5[prime]-splice junction of intron 3 in the six-intron pre-mRNA. Northern blot analysis, genomic and cDNA sequencing, and primer extension analysis indicated that the mutation causes inefficient and incomplete splicing of the pre-mRNA, resulting in the accumulation of three aberrant mRNAs. One mutant mRNA was identical with wild-type mRNA except that it included intron 3, a second mRNA comprised intron 3 and exons 4 through …
Porphyrin Accumulation And Export By Isolated Barley (Hordeum Vulgare) Plastids (Effect Of Diphenyl Ether Herbicides), Judith M. Jacobs, Nicholas J. Jacobs
Porphyrin Accumulation And Export By Isolated Barley (Hordeum Vulgare) Plastids (Effect Of Diphenyl Ether Herbicides), Judith M. Jacobs, Nicholas J. Jacobs
Dartmouth Scholarship
We have investigated the formation of porphyrin intermediates by isolated barley (Hordeum vulgare) plastids incubated for 40 min with the porphyrin precursor 5-aminolevulinate and in the presence and absence of a diphenylether herbicide that blocks protoporphyrinogen oxidase, the enzyme in chlorophyll and heme synthesis that oxidizes protoporphyrinogen IX to protoporphyrin IX. In the absence of herbicide, about 50% of the protoporphyrin IX formed was found in the extraplastidic medium, which was separated from intact plastids by centrifugation at the end of the incubation period. In contrast, uroporphyrinogen, an earlier intermediate, and magnesium protoporphyrin IX, a later intermediate, were located mainly …