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Evidence Against Equimolarity Of Large Repeat Arrangements And A Predominant Master Circle Structure Of The Mitochondrial Genome From A Monkeyflower (Mimulus Guttatus) Lineage With Cryptic Cms, Jeffrey P. Mower, Andrea L. Case, Eric R. Floro, John H. Willis

Center for Plant Science Innovation: Faculty and Staff Publications

Despite intense investigation for over 25 years, the in vivo structure of plant mitochondrial genomes remains uncertain. Mapping studies and genome sequencing generally produce large circular chromosomes, whereas electrophoretic and microscopic studies typically reveal linear and multi-branched molecules. To more fully assess the structure of plant mitochondrial genomes, the complete sequence of the monkeyflower (Mimulus guttatus DC. line IM62) mtDNA was constructed from a large (35 kb) paired-end shotgun sequencing library to a high depth of coverage (~30x). The complete genome maps as a 525,671 bp circular molecule and exhibits a fairly conventional set of features including 62 genes …