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The Dna- And Rna-Binding Protein Factor Of Dna Methylation 1 Requires Xh Domain-Mediated Complex Formation For Its Function In Rna-Directed Dna Methylation, Meng Xie, Guodong Ren, Chi Zhang, Bin Yu
The Dna- And Rna-Binding Protein Factor Of Dna Methylation 1 Requires Xh Domain-Mediated Complex Formation For Its Function In Rna-Directed Dna Methylation, Meng Xie, Guodong Ren, Chi Zhang, Bin Yu
Center for Plant Science Innovation: Faculty and Staff Publications
Studies have identified a sub-group of SGS3-LIKE proteins including FDM1–5 and IDN2 as key components of RNA-directed DNA methylation pathway (RdDM). Although FDM1 and IDN2 bind RNAs with 5' overhangs, their functions in the RdDM pathway remain to be examined. Here we show that FDM1 interacts with itself and with IDN2. Gel filtration suggests that FDM1 may exist as a homodimer in a heterotetramer complex in vivo. The XH domain of FDM1 mediates the FDM1–FDM1 and FDM1–IDN2 interactions. Deletion of the XH domain disrupts FDM1 complex formation and results in loss-of-function of FDM1. These results demonstrate that XH domainmediated …
Critical Roles Of Rna-Binding Proteins In Mirna Biogenesis In Arabidopsis, Guodong Ren, Bin Yu
Critical Roles Of Rna-Binding Proteins In Mirna Biogenesis In Arabidopsis, Guodong Ren, Bin Yu
Center for Plant Science Innovation: Faculty and Staff Publications
MicroRNAs (miRNAs) are key regulators of gene expression and play critical roles in modulating metabolism, development and physiology in animals and plants. miRNA levels are transcriptionally and post-transcriptionally controlled for their proper function. Recent studies have shown that RNA-binding proteins play important roles in producing miRNAs by affecting the accurate and/or efficient processing of precursors of miRNAs. Many of these RNA-binding proteins also have roles in general RNA metabolism, indicating potential connections between miRNA biogenesis and other RNA metabolism. Here, we focus on the function of several RNA-binding proteins in miRNA biogenesis in Arabidopsis.