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Functional Annotation, Transcriptional Characterization And Enzymatic Contributions Of Essential Amino Acid Biosynthesis-Related Genes, Teresa J. Clark
Functional Annotation, Transcriptional Characterization And Enzymatic Contributions Of Essential Amino Acid Biosynthesis-Related Genes, Teresa J. Clark
Masters Theses
In plants, essential amino acid biosynthesis predominantly or exclusively occurs in the plastid. The plastid in the heterokont alga Nannochloropsis oceanica is surrounded by four membranes, which add great complexity to intracellular trafficking and communication. N. oceanica genes in essential amino acid biosynthesis were functionally annotated. The biosynthesis pathways resemble the pathways in Arabidopsis thaliana, but the gene content seems to be simpler in N. oceanica.
In addition, two A. thaliana mutants with loss-of-function mutations in the aspartate kinase-homoserine dehydrogenase 2 (AK-HSDH2) gene were characterized. These ak-hsdh2 mutants demonstrate unexpected accumulation of aspartate-derived amino acids (ADAAs), particularly threonine, in …