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Aversion Properties Of Retrorsine, Retrorsine Nitrogen Oxide And Frosted Senecio Latifolius, Leendert D. Snyman Dec 2023

Aversion Properties Of Retrorsine, Retrorsine Nitrogen Oxide And Frosted Senecio Latifolius, Leendert D. Snyman

Poisonous Plant Research (PPR)

Two pilot trials, investigating the aversive properties of retrorsine and retrorsine nitrogen oxide and that of frosted Senecio latifolius, were executed. Retrorsine and retrorsine-NO (the toxic principles of Senecio retrorsus), unlke sceleratine-NO (the toxic principle of another hepatotoxic pyrrolizidine alkaloid containing plant, namely S. latifolius), were not capable of establishing aversion when given to a sheep. Frosted S. latifolius given to a sheep also did not induce aversion. However, a sheep averted to S. latifolius (non-frosted) refused eating frosted S. latifolius.


Sceleratin Nitrogen Oxide As Aversive Agent In Conditioning Livestock To Avoid Senecio Latifolius, Leendert D. Snyman Dec 2023

Sceleratin Nitrogen Oxide As Aversive Agent In Conditioning Livestock To Avoid Senecio Latifolius, Leendert D. Snyman

Poisonous Plant Research (PPR)

Sceleratine nitrogen oxide, when administered together with a dichloromethane extract of Senecio latifolius, successfully conditioned cattle and sheep to avoid milled freeze dried S. latifolius mixed with maize meal. This treatment was effectively applied in conditioning steers to refuse eating S. latifolius grown in pots.


Isolation Of The Toxic Principle Of Senecio Latifolius By Means Of The Sensory Receptors Of Sheep, Leendert D. Snyman Dec 2023

Isolation Of The Toxic Principle Of Senecio Latifolius By Means Of The Sensory Receptors Of Sheep, Leendert D. Snyman

Poisonous Plant Research (PPR)

The aversive substance of Senecio latifolius was isolated by means of the sensory receptors of sheep averted to S. latifolius. Chemical fractions refused due to the presence of the aversive substance sensed by the sheep were fractionated until a purified substance had been isolated. Nuclear magnetic resonance (NMR) analysis of the purified substance showed it to be sceleratine nitrogen oxide, the toxic principle of S. latifolius.


Fruits Of Nandina Domestica Are (Sometimes) Cyanogenic And (Sometimes) Hazardous To Birds, Scott Zona Apr 2022

Fruits Of Nandina Domestica Are (Sometimes) Cyanogenic And (Sometimes) Hazardous To Birds, Scott Zona

Poisonous Plant Research (PPR)

A 12-month survey of cyanogenesis in the fruits and leaves of Nandina domestica (Berberidaceae) revealed that the leaves are strongly cyanogenic throughout the year, as measured by the Feigl-Anger test. Fruits vary in their cyanogenic potential depending on the degree of ripeness and clone. Green fruits are strongly and rapidly cyanogenic, but most ripe fruits are weakly and slowly cyanogenic. Some fruits tested negative for cyanide. Fruits tend to become less cyanogenic the longer they remain on the plant, so birds feeding on older fruits are at a lower risk of poisoning. More than other frugivores, cedar waxwings (Bombycilla …


Rapid Quantitative Analysis Of Toxic Norditerpenoid Alkaloids In Larkspur (Delphinium Spp.) By Flow Injection - Electrospray Ionization – Mass Spectrometry, Dale R. Gardner, Stephen T. Lee, Daniel Cook Sep 2021

Rapid Quantitative Analysis Of Toxic Norditerpenoid Alkaloids In Larkspur (Delphinium Spp.) By Flow Injection - Electrospray Ionization – Mass Spectrometry, Dale R. Gardner, Stephen T. Lee, Daniel Cook

Poisonous Plant Research (PPR)

A rapid flow injection - electrospray ionization – mass spectrometry (FI-ESI-MS) method for the quantitative and qualitative analysis of norditerpenoid alkaloids in larkspur plants was developed. The FI-ESI-MS method was calibrated for alkaloid concentrations with larkspur plant samples against an existing Fourier transform infrared spectroscopy FTIR method. There was a strong correlation (r = 0.9926, r = 0.9891) between the FTIR and FI-ESI-MS methods. The relative standard deviation (RSD) for all measurements were ≤ 6.2 % except for the sample with the lowest concentration which was 19%. The sample throughput of the FI-ESI-MS method is much higher than the FTIR …


Aconitum Lycoctonum L.: Phenolic Compounds And Their Bioactivities, Erna Karalija, Adisa Paric, Sabina Dahija, Renata Besta Gajevic, Sanja Cavar Zeljkovic May 2021

Aconitum Lycoctonum L.: Phenolic Compounds And Their Bioactivities, Erna Karalija, Adisa Paric, Sabina Dahija, Renata Besta Gajevic, Sanja Cavar Zeljkovic

Poisonous Plant Research (PPR)

The very first report on the phenolic composition of aerial parts of Aconitum lycoctonum L., a species belonging to the toxic Aconitum genus, is presented here. Aerial parts were subjected to the extraction with four different solvents and analyzed via LC-MS/MS for the content of phenolic acids and flavonoids. Furthermore, isolated extracts were tested for antimicrobial and antioxidant activities. Ethanolic extracts of both flowers and vegetative parts (leaves and stems) were found to be the richest in the phenolic compounds, following the water extracts. Ethanolic extract of flowers was very rich in flavonoid apigenin, while high levels of salicylic and …


An Evaluation Of The Susceptibility Of Goats To Larkspur Toxicosis, Kevin D. Welch, Clint A. Stonecipher, Dale R. Gardner, Benidict T. Green, Daniel Cook Apr 2020

An Evaluation Of The Susceptibility Of Goats To Larkspur Toxicosis, Kevin D. Welch, Clint A. Stonecipher, Dale R. Gardner, Benidict T. Green, Daniel Cook

Poisonous Plant Research (PPR)

Larkspurs (Delphinium spp.) are a major cause of cattle losses in western North America, whereas sheep have been shown to be resistant to larkspur toxicosis. Goats are often used as a small ruminant model to study poisonous plants, even though they can be more resistant to some poisonous plants. It is not known how susceptible goats are to the adverse effects of larkspurs. In this study, we evaluated the susceptibility of goats to larkspur toxicosis by performing a dose-response study. We dosed goats with D. barbeyi collected near Cedar City, Utah at 3.3, 4.4, 6.6, 8.8 and 10.0 g …


Molecular Differentiation Of Astragalus Species And Varieties From The Western United States: The Chloroplast Dna Bridge Between Evolution And Molecular Systematics, Marwa Neyaz, Daniel Cook, Rebecca Creamer Mar 2020

Molecular Differentiation Of Astragalus Species And Varieties From The Western United States: The Chloroplast Dna Bridge Between Evolution And Molecular Systematics, Marwa Neyaz, Daniel Cook, Rebecca Creamer

Poisonous Plant Research (PPR)

Locoweeds are the most widespread poisonous plant problem in the world and have been reported in the Western United States since the 1800s, causing tremendous losses in livestock. Consumption of locoweeds by grazing animals stimulates the neurological disease, locoism, characterized by weight loss, ataxia, and lack of muscular coordination. The name locoweed is used for Astragalus and Oxytropis species known to contain swainsonine, the toxic principle produced by the plant endophytic fungus Undifilum. Astragalus includes 2,500-3,000 species and many varieties that have almost identical morphological characteristics that overlap among species, leading to improper identification. Therefore, the aim of this study …