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Quantifying The Variability In Heavy Metal Concentrations In Produce Grown In Metals-Rich Soil, Harris Lowell Byers Dec 2019

Quantifying The Variability In Heavy Metal Concentrations In Produce Grown In Metals-Rich Soil, Harris Lowell Byers

Theses and Dissertations

Childhood Pb exposure is associated with a multitude of poor health outcomes. In food-insecure areas, growing fresh produce in residential backyard gardens is one option for parents; however, commonly grown crops are known to accumulate Pb in consumable tissues when grown in metals-rich soils. A variety of produce representing a continuum of consumable tissues were grown in soils collected from two residential vegetable gardens, a former metal foundry, and commercial topsoil purchased from a local hardware store. The concentrations of heavy metals in crop tissues were measured with custom wavelength dispersive X-ray Fluorescence (WD-XRF) spectroscopy and portable energy dispersive X-ray …


A Seascape Genetics Approach To Studying Genetic Differentiation In The Bull Kelp Nereocystis Luetkeana, Lily G. Gierke Dec 2019

A Seascape Genetics Approach To Studying Genetic Differentiation In The Bull Kelp Nereocystis Luetkeana, Lily G. Gierke

Theses and Dissertations

The brown alga Nereocystis luetkeana is a foundation species found from Alaska to California. In the Salish Sea, N. luetkeana is declining, but little is known about its population structure. We explored N. luetkeana 1) allelic dissimilarity and richness using seven microsatellite markers, and 2) tested models of gene flow in the Salish Sea using a hydrodynamic transport model. Our results suggest that the N. luetkeana distribution is comprised of four genetic co-ancestry groups that are geographically coherent, apart from the separation of the Strait of Georgia/Puget Sound by the Strait of Juan de Fuca. Our model supported that environmental …


Exploring Primitive Legume Symbiosis Using Chamaecrista Fasciculata As A Model, Zachary E. Zawada Aug 2019

Exploring Primitive Legume Symbiosis Using Chamaecrista Fasciculata As A Model, Zachary E. Zawada

Theses and Dissertations

Legumes form symbiotic associations with diazotrophs collectively termed “rhizobia” leading to the formation of nodules and N2-fixation and contribute significant amounts of fixed N to agricultural and natural environments. The mechanisms of nodulation and N2-fixation are extensively studied using model legumes that belong to the Papilionoideae, in which nodulation is widespread and advanced. In contrast, very little is known about the mechanisms of nodulation and N2-fixation in in basal Caesalpinioideae subfamily in which nodulation is rare. The nodulating caesalpiniod legume Chamaecrista fasciculata is widespread in North America and is suggested as a complementary model to study legume nodulation because of …


Dissection Of Floral Organ Development And Sterility In Sorghum Bicolor, Ashley R. Smith Aug 2019

Dissection Of Floral Organ Development And Sterility In Sorghum Bicolor, Ashley R. Smith

Theses and Dissertations

Sorghum bicolor is a drought resistant cereal grain commonly grown for use in food, feed, fiber, and fuel production. Due to its versatility and modest sized genome it is poised to become an increasingly important research organism within the C4 plants. Sorghum produces spikelet pairs with one fertile sessile spikelet and one to two sterile pedicellate spikelets. One major area of interest in sorghum production is maximizing seed yield. While the importance of the grain is obvious there are several large gaps in the study of sorghum that prevent researchers and growers from maximum productivity. The first gap is that …


Lorelei Localization And Ovule Ultrastructure In Arabidopsis Thaliana, Juleen May Dickson Aug 2019

Lorelei Localization And Ovule Ultrastructure In Arabidopsis Thaliana, Juleen May Dickson

Theses and Dissertations

Communication between the male and female gametophyte is vital to successful fertilization during sexual reproduction in plants. One of the proteins known to be important for communication between the male and female gametophyte is LORELEI (LRE). Several studies have shown that there are defects in pollen tube guidance and synergid degeneration, however this is the first study that shows that cell wall thickness in the female gametophyte may also be affected. Previous confocal studies have documented that LRE is present both in the filiform apparatus and found in puncta throughout the cytoplasm. This study confirmed this, but our studies suggest …