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Construction Of Crispr/Cas9 Vectors For Directed Mutagenesis Of Fusarium Graminearum, Rebekah Akers
Construction Of Crispr/Cas9 Vectors For Directed Mutagenesis Of Fusarium Graminearum, Rebekah Akers
Honors Program Theses
Fusarium graminearum causes the fungal disease known as scab or head blight in cereals, including barley. There is no known resistance in barley against scab disease. Generating mutations in the fungus is a promising strategy to reduce infection. Research has shown that CRISPR/Cas9 is a very powerful tool for gene mutation. In this study, CRISPR/Cas9 vectors containing guide RNA sequences were constructed to mutate one visual marker gene (AUR1) and two genes essential for infection in F. graminearum (Tri5 and MGV1). Plasmids pFC332 and pFC334 were used to create vectors with the target genes using the …
The Effect Of Cold Stratification And Perigynia Removal On The Germination Of Three Prairie Carex Species, Christina M. Boeck Crew
The Effect Of Cold Stratification And Perigynia Removal On The Germination Of Three Prairie Carex Species, Christina M. Boeck Crew
Dissertations and Theses @ UNI
Sedges (Carex spp. Linneaus. [Cyperaceae]) are a major component of Midwestern U.S. tallgrass prairie and prairie wetland plant communities, yet they are often lacking in restoration projects because they can be difficult to germinate. Cold-wet stratification and perigynia removal have been shown to increase germination in some Carex species. The germination response of 3 native species, plains oval or shortbeak sedge (C. brevior (Dewey) Mack.), field oval or troublesome sedge (C. molesta Mack.), and prairie or Bicknell’s sedge (C. bicknellii Britton.), was tested to cold-wet stratification (28d) and perigynia removal in stored seed (10 months). Seed treatments …
Morphological And Morphometric Analysis Of Nekemias Arborea And Ampelopsis Aconitifolia (Vitaceae), Sally Rose Gray
Morphological And Morphometric Analysis Of Nekemias Arborea And Ampelopsis Aconitifolia (Vitaceae), Sally Rose Gray
Dissertations and Theses @ UNI
Leaf morphology in angiosperms is not constrained by the leaf’s important function of providing energy through photosynthetic reactions. In just one family, Vitaceae, it is easy to observe among it’s over 900 species, various leaf shapes and sizes even among closely related species as well as within individual species. Observation of these leaf forms within species can be used to determine, for example where a vine transitions from the juvenile state to the adult state as well as their relationship to the spatial and temporal patterning of inflorescence initiation. Analysis of two species within the Ampelopsis clade, one that retained …