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Movement Of Dna Across The Chloroplast Envelope: Implications For The Transfer Of Promiscuous Dna, Heriberto D. Cerutti, André Jagendorf

Center for Plant Science Innovation: Faculty and Staff Publications

Little is known about the mechanistic basis for the movement of promiscuous nucleic acids across cell membranes. To address this problem we sought conditions that would permit the entry of plasmid DNA into isolated, intact pea chloroplasts. DNA uptake did not occur normally, but was induced by hypotonic treatments, by incubation with millimolar levels of Mg²⁺, or by heat shock at 42 °C. These results are consistent with DNA movement being permitted by conditions that transiently alter the permeability of the chloroplast envelope. Plant cells are subject to osmotic tensions and/or conditions inducing polymorphic changes in the membranes, …