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Use Of Shoot-Root Grafts In Case Studies Of Site Of O-Hydroxycinnamic Acid Synthesis In Melilotus Alba, Herman J. Gorz, Francis A. Haskins Nov 1966

Use Of Shoot-Root Grafts In Case Studies Of Site Of O-Hydroxycinnamic Acid Synthesis In Melilotus Alba, Herman J. Gorz, Francis A. Haskins

Department of Agronomy and Horticulture: Faculty Publications

The content of o-hydroxycinnamic acid was measured in shoot-root grafts involving four combinations of Melilotus alba plants of the CuCuBB and cucuBB genotypes. Six portions from each grafted plant were assayed. Results provide evidence that o-hydroxycinnamic acid synthesis occurs in the above-ground parts of sweetclover plants, with limited translocation to the roots. No support was obtained for the view that the root plays an important role in o-hydroxycinnamic acid synthesis.


Cultural Practices For Seed Production From Established Stands Of Western Wheatgrass, D. E. Smika, L. C. Newell Jun 1966

Cultural Practices For Seed Production From Established Stands Of Western Wheatgrass, D. E. Smika, L. C. Newell

Nebraska Agricultural Experiment Station: Historical Research Bulletins

Fertilization and irrigation requirements and aftermath removal practices for maximum seed production from solid stand western wheatgrass were studied for three years near Oakley, Kansas. Conclusions from this study indicate that to obtain maximum quantity of highest quality western wheatgrass seed from established stands, the grass must be fertilized in the fall with at least 80 pounds of nitrogen per acre. Irrigation with sufficient water to fill the root zone in either the fall or early spring and again when the grass is in " boot" or heading is necessary. The practice of removing the aftermath following harvest by mowing …


Changes In Fungi With Age I. Chemical Composition Of Rhizoctonia Solani And Sclerotium Bataticola , David Gottlieb, James L. Van Etten Jan 1966

Changes In Fungi With Age I. Chemical Composition Of Rhizoctonia Solani And Sclerotium Bataticola , David Gottlieb, James L. Van Etten

Department of Plant Pathology: Faculty Publications

The chemical composition of the mycelium of Rhizoctonia solani and Sclerotium bataticola was determined in cells of various ages. The percentage, per unit of dry weight, of soluble amino nitrogen, deoxyribonucleic acid (DNA), ribonucleic acid (RNA), ergosterol, and protein decreased with age in both fungi. Total lipids and fatty acids increased with age in S. bataticola but remained constant in R. solani. Total carbohydrate increased with age in R. solani and decreased in S. bataticola. Fewer changes with age were observed when the results were calculated in ratio to DNA. There was no change in the ratios of protein, …


Changes In Fungi With Age Ii. Respiration And Respiratory Enzymes Of Rhizoctonia Solani And Sclerotium Bataticola, James L. Van Etten, H. Peter Molitoris, David Gottlieb Jan 1966

Changes In Fungi With Age Ii. Respiration And Respiratory Enzymes Of Rhizoctonia Solani And Sclerotium Bataticola, James L. Van Etten, H. Peter Molitoris, David Gottlieb

Department of Plant Pathology: Faculty Publications

The rate of respiration of Rhizoctonia solani and Sclerotium bataticola decreased with age. This decrease in respiratory rate might be produced by a decrease in the specific activity of one or more enzymes involved in carbohydrate metabolism. Specific activities in cell-free extracts were measured for most of the enzymes in the hexose monophosphate shunt, Embden-Meyerhof-Parnas pathway, tricarboxylic acid cycle, and terminal electron-transport system. In addition, glucose oxidase, isocitritase, and malic enzyme were measured. In R. solani, increases in activity with age occurred for hexokinase, α-glycerolphosphate dehydrogenase, malic dehydrogenase, and cytochrome oxidase. Decreases occurred for phosphohexokinase, aconitase, nicotinamide adenine dinucleotide-specific …


Growing Proso In Nebraska, P. H. Grabouski Jan 1966

Growing Proso In Nebraska, P. H. Grabouski

Nebraska Agricultural Experiment Station: Historical Circulars

Proso, sometimes called "hog millet," is receiving attention in the Nebraska Panhandle as a dryland grain crop because of acreage restrictions and limited crop alternatives.


Genetic And Biochemical Aspects Of O-Hydroxycinnamic Acid Synthesis In Melilotus Alba, Herman J. Gorz, Francis A. Haskins, A. Kleinhofs Jan 1966

Genetic And Biochemical Aspects Of O-Hydroxycinnamic Acid Synthesis In Melilotus Alba, Herman J. Gorz, Francis A. Haskins, A. Kleinhofs

Department of Agronomy and Horticulture: Faculty Publications

In sweetclover (Melilotus alba Desr.) the cis- and trans-isomers of o-hydroxycinnamic acid occur primarily as the respective, β-D-glucosides. Available evidence indicates that these glucosides are formed via the following pathway: phenylalanine (formed from shikimic acid) → trans-cinnamic acid → o-coumaric acid (trans-o-hydroxycinnamic acid) → o-coumaryl glucoside (trans-β-D-glucosyl-o-hydroxycinnamic acid) → coumarinyl glucoside (cis-β-D-glucosyl-o-hydroxycinnamic acid). In tissues that are disrupted, coumarinyl glucoside is rapidly hydrolyzed by the action of endogenous β-glucosidase to yield coumarinic acid, which lactonizes spontaneously to form coumarin. The cu …