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Human Calmodulin Methyltransferase: Expression, Activity On Calmodulin, And Hsp90 Dependence, Sophia Magen, Roberta Magnani, Sitvanit Haziza, Eli Hershkovitz, Robert Houtz, Franca Cambi, Ruti Parvari

Horticulture Faculty Publications

Deletion of the first exon of calmodulin-lysine N-methyltransferase (CaM KMT, previously C2orf34) has been reported in two multigene deletion syndromes, but additional studies on the gene have not been reported. Here we show that in the cells from 2p21 deletion patients the loss of CaM KMT expression results in accumulation of hypomethylated calmodulin compared to normal controls, suggesting that CaM KMT is essential for calmodulin methylation and there are no compensatory mechanisms for CaM methylation in humans. We have further studied the expression of this gene at the transcript and protein levels. We have identified 2 additional …

Forage News [2012-12], Department Of Plant And Soil Sciences, University Of Kentucky

Forage News

• Farm Bureau Forage Commodity Conference
• AFGC will hold its Annual Meeting this January in Kentucky
• Forages at KCA
• Kentucky Small Ruminant Grazing Conference
• Heart of America Grazing Conference
• Choose Forage Varieties Wisely
• Hay Testing - A Wise Investment
• Energy-Crop Calculator Available Online
• Strong Hay Prices through Winter Maybe Longer
• Dairy Herd Expansion Unlikely in 2013
• What is Really Important?

Intraspecific Diversity In Sinningia Speciosa (Gesneriaceae: Sinningieae), And Possible Origins Of The Cultivated Florist's Gloxinia, David Zaitlin

Kentucky Tobacco Research and Development Center Faculty Publications

BACKGROUND AND AIMS: The florist's gloxinia is a familiar houseplant in the Gesneriaceae, the botanical family that includes the African violet (Saintpaulia) and other ornamental species. The gloxinia's wild progenitor is Sinningia speciosa (Lodd.) Hiern, a Brazilian endemic. Although it has been cultivated for almost 200 years, little is known about the genetic diversity in S. speciosa, how the wild populations relate to one another or even where the cultivated forms originated. Using available wild collections, preliminary phenetic and phylogenetic investigations were conducted to elucidate the interspecific relationships within S. speciosa and to infer the origins of the cultivars.

METHODOLOGY: …

Morphological And Genetic Changes Induced By Excess Zn In Roots Of Medicago Truncatula A17 And A Zn Accumulating Mutant, Ricky W. Lewis, Guiliang Tang, David H. Mcnear

Plant and Soil Sciences Faculty Publications

BACKGROUND: Nutrient fluxes associated with legume-rhizobia symbioses are poorly understood and little is known regarding the influence of abiotic stresses on development and maintenance of N-fixing nodules and root system architecture (RSA). We examined effects of Zn on nodule development and structure, root architecture, and expression of nodulation-related miRNAs in Medicago truncatula and the mutant, raz (requires additional Zn).

FINDINGS: Excess Zn increased root and shoot associated Zn in both genotypes, however, raz plants had lower root associated Zn than WT plants. Roots of raz plants exposed to excess Zn had less volume, surface area, and total length compared to …

Plant Polyadenylation Factors: Conservation And Variety In The Polyadenylation Complex In Plants, Arthur G. Hunt, Denghui Xing, Qingshun Q. Li

Plant and Soil Sciences Faculty Publications

BACKGROUND: Polyadenylation, an essential step in eukaryotic gene expression, requires both cis-elements and a plethora of trans-acting polyadenylation factors. The polyadenylation factors are largely conserved across mammals and fungi. The conservation seems also extended to plants based on the analyses of Arabidopsis polyadenylation factors. To extend this observation, we systemically identified the orthologs of yeast and human polyadenylation factors from 10 plant species chosen based on both the availability of their genome sequences and their positions in the evolutionary tree, which render them representatives of different plant lineages.

RESULTS: The evolutionary trajectories revealed several interesting features of plant polyadenylation factors. …

Forage News [2012-11], Department Of Plant And Soil Sciences, University Of Kentucky

Forage News

• Kentucky Grazing Conference
• KFGC Presents Awards
• Alfalfa Intensive Training Seminar
• Kentuckian Featured at National Hay Convention
• AFGC Conference in Covington
• Kentucky's 2012 National Forage Spokesman to speak at Farm Bureau Forage Commodity Conference
• Dr. Peter Ballerstedt to Speak at Forages at KCA During the KCA Convention
• Neutral Detergent Fiber Digestibility (NDFD): A Good Predictor of Forage Quality
• Where Does Nitrogen Fertilizer Come From?
• The Cost of Pasture vs. Hay

Strategies To Minimize The Effects Of Drought, Gary Bates

Kentucky Grazing Conference

As this summer has shown, forage production from cool-season pasture and hay fields across the state can be dramatically affected by drought. Pastures that consist of cool-season grasses like tall fescue and orchardgrass have been severely overgrazed, and many may have lost some significant stand. Even if you have reseeded these fields, the question is how to prevent having the same problem next year. One of the best practices is to develop a forage program that doesn’t rely on cool-season grasses for summer production, but selects forage species that are better able to produce during periods of limited rainfall and …

Grassfed Beef From A Global Perspective, Glen Aiken

Kentucky Grazing Conference

Beef production and marketing has been shifting over the last few decades in response to unstable cattle markets, higher input costs, and a need to better meet market demands. Advancement of vertical integration of the production and marketing of beef calves through retained ownership from suckling calf to feedyard has certainly enhanced the genetics of the U.S. beef herd and improved the consistency of beef products. Another shift in production has been an increased number of grassfed cattle (grass finished) for marketing either organic or locally grown beef. These niche markets have developed primarily from concerns of urban consumers regarding …

The Cost Of Pasture Versus Hay, Kenneth H. Burdine

Kentucky Grazing Conference

Whenever I discuss the economics of grazing, I like to start by thinking about hay production costs. Regardless of which species we are talking about, a livestock producer should know what his / her most expensive feeding days are. In most cases, these will be winter feeding days, and that will generally consist of feeding either purchased or home-grown hay. So, I encourage producers to start by getting a solid feel for what their daily winter feeding costs are. For the purposes of this article, I am going to present some specific estimates for winter feeding costs, and other items, …

Pastures For Horses, Robert Coleman

Kentucky Grazing Conference

For horse owners, the use of pasture in the daily management of their horses provides both exercise for the horses, but also is a valuable source of nutrients. Horse feeding programs are based on forage and pasture is an excellent means to supply nutrients. To make effective use of the forage, horse owners need to manage both the horse and the grass.

Winter Annuals And Short Term Perennials For Grazing, S. Ray Smith

Kentucky Grazing Conference

During the last 5 years there has been a renewed interest in winter annual and short term perennials for grazing and for stored feed. There are many sources of information on this subject. I especially encourage you to read the new publication written by Dr. Garry Lacefield and colleagues, “Extending Grazing and Reducing Stored Feed Needs”. I have used information from this publication and other publications from the University of Kentucky Forage Website (www.uky.edu/Ag/Forage) in writing this article. This website contains many use publications on the subject of winter annuals and variety trial information on annual ryegrass and …

Novel Endophyte Tall Fescue, Garry D. Lacefield

Kentucky Grazing Conference

“Novel” endophyte is a term used to describe a “good” or “friendly” fungus (endophyte) in tall fescue as opposed to the endophyte that’s present if over eighty-five percent of the tall fescue in the Fescue Belt of the USA. This endophyte is costing beef producers an estimated $1 Billion dollars in losses from animal gains, reproduction and overall performance.

Forages On My Farm, Brent White

Kentucky Grazing Conference

No abstract provided.

Foreword And Kfgc Award Winners [2012], Garry D. Lacefield, Christi L. Forsythe

Kentucky Grazing Conference

No abstract provided.

Effects Of Long-Term Cattle Grazing And Woody Plant Encroachment On Soil Microbial Communities At The Santa Rita Experimental Range, Arizona, Cody Burton, Steven Archer, Rebecca L. Mcculley

Plant and Soil Sciences Presentations

Livestock grazing is considered a key driver of woody plant encroachment in dryland ecosystems worldwide. Woody plant establishment in these systems creates “islands of fertility,” in part by modifying erosional processes such that soil and plant litter are deposited beneath the shrub canopy, creating a nutrient rich soil/litter matrix that supports enhanced soil microbial biomass pools. In this study, we utilized a long-term grazing exclosure (>80 yrs) at the Santa Rita Experimental Range south of Tucson, Arizona and phopsholipid fatty acid analysis to quantify livestock grazing effects on soil microbial communities associated with the complex vegetative mosaic that characterizes …

Forage News [2012-10], Department Of Plant And Soil Sciences, University Of Kentucky

Forage News

• 13th Kentucky Grazing Conference
• AFGC Returns to Kentucky - Register Soon
• 33rd Kentucky Alfalfa Conference
• Heart of America Grazing Conference
• Hay Testing - A Wise Investment
• UK Beef Cow Forage Supplement Tool - A Web Based APP
• KFGC Field Day
• Kentucky Grazing School
• Bale Weight, Nutrient Removal, and Feed Value of Hay: An On-Farm Perspective
• Beware of Cyanide (Prussic Acid) Poisoning
• Nitrate Rumors can Kill Cattle

Cytochrome P450s And Uses Thereof, Joe Chappell, Lyle F. Ralston

Plant and Soil Sciences Faculty Patents

The invention features isolated cytochrome P450 polypeptides and nucleic acid molecules, as well as expression vectors and transgenic plants containing these molecules. In addition, the invention features uses of such molecules in methods of increasing the level of resistance against a disease caused by a plant pathogen in a transgenic plant, in methods for producing altered compounds, for example, hydroxylated compounds, and in methods of producing isoprenoid compounds.

Forage News [2012-09], Department Of Plant And Soil Sciences, University Of Kentucky

Forage News

• KFGC Forage Field Day
• Kentucky Grazing School
• Beef Bash 2012
• National Hay Association to Feature Kentucky Speakers
• 13th Kentucky Grazing Conference
• Partridge Pea or Chamaecrista fasciculate (Michx.) Greene
• Forage Brassica Crops in Grazing Systems
• Darkling/Mealworm Beetles can cause "Blister Beetle Scare"

Forage News [2012-08], Department Of Plant And Soil Sciences, University Of Kentucky

Forage News

• KFGC Field Day - Hart County - September 6
• Drought and Forages
• Choose the Best Variety for Fall Plantings
• Plan to Attend the AFGC Meeting in Covington, KY - Jan. 7-9, 2013
• High Nitrates Especially in Drought Stressed Corn
• Biofuels will Continue to Drive Feed Prices
• Mother Nature Throws a Wrench into 2012

Discovery Of Novel Dsrna Viral Sequences By In Silico Cloning And Implications For Viral Diversity, Host Range And Evolution, Huiquan Liu, Yanping Fu, Jiatao Xie, Jiasen Cheng, Said A. Ghabrial, Guoqing Li, Xianhong Yi, Daohong Jiang

Plant Pathology Faculty Publications

Genome sequence of viruses can contribute greatly to the study of viral evolution, diversity and the interaction between viruses and hosts. Traditional molecular cloning methods for obtaining RNA viral genomes are time-consuming and often difficult because many viruses occur in extremely low titers. DsRNA viruses in the families, Partitiviridae, Totiviridae, Endornaviridae, Chrysoviridae, and other related unclassified dsRNA viruses are generally associated with symptomless or persistent infections of their hosts. These characteristics indicate that samples or materials derived from eukaryotic organisms used to construct cDNA libraries and EST sequencing might carry these viruses, which were not easily detected by the researchers. …

Reproductive Characteristics Of A Populus Euphratica Population And Prospects For Its Restoration In China, Dechang Cao, Jingwen Li, Zhenying Huang, Carol C. Baskin, Jerry M. Baskin, Peng Hao, Weilei Zhou, Junqing Li

Biology Faculty Publications

Populus euphratica is a dominant tree in riparian ecosystems in arid areas of northwest China, but it fails to regenerate in these systems. This study evaluates causes for the failure of sexual and asexual regeneration of this species in the wild. P. euphratica disperses as many as 85743 seeds/m² during summer, and the seeds germinate to 92.0% in distilled water and to 60.8% on silt. However, very few seeds (3.6%) can germinate on unflooded soil. The seed-rain season is prolonged by temporal variability in seed dispersal among individuals, which ensures that seedling emergence can occur during favorable conditions (i.e., …

Utility Of Phylloplanins As Antibiotics, Selective Fungicides And For Enhancing Microbial Resistance In Plants, George Wagner, Ryan Sheperd

Plant and Soil Sciences Faculty Patents

The present invention relates to plant proteins produced by a plant's epidermal layer that contribute to the innate pest/disease resistance of the plant (“phylloplanins”), compositions comprising the phylloplanins and methods of using them. In particular the methods relate to inhibiting or preventing microbial, e.g., fungal or bacterial, growth on a subject, organism or surface by administering a phylloplanin. The invention also relates to transformed host cells that produce phylloplanins, and to transgenic plants producing phylloplanins conferring increased resistance to microbial infections/growth.

Forage News [2012-07], Department Of Plant And Soil Sciences, University Of Kentucky

Forage News

• Drought and Forages
• Kentucky Hay Grower Wins National Hay Awards
• KFGC Field Day to be in Hart County
• Native Warm-Season Grasses In-Service Training
• AFGC National Tour
• If Baling Hay Wet, Watch for Fires
• Custom Grazing of Stocker Cattle
• AFGC 2013 Annual Conference
• AFGC 2013 National Competitions
• Reducing Energy Use in Production Agriculture
• Ohio State Alfalfa Enterprise Budgets Online
• The Diverse Structure and Organization of U.S. Beef Cow-Calf Farms

Differential Expression Of P1f1-Targeted Genes In Various Pif1 And Ctg10 Mutants, Taylor D. Lloyd

Kaleidoscope

No abstract provided.

Plants Having An Enhanced Resistance To Necrotrophic Pathogens And Method Of Making Same, Pradeep Kachroo, Aardra Kachroo

Plant Pathology Faculty Patents

A method for enhancing resistance to necrotrophic and/or hemibiotrophic pathogens by overexpressing glycerol-3-phosphate dehydrogenase using an expression vector in a plant species. For example, the present method can be used to enhance resistance to C. higginsianum by overexpressing glycerol-3-phosphate dehydrogenase in a plant such as Arabidopsis plant, using an expression vector in a plant.

Evolutionary Genomics Of Mycovirus-Related Dsrna Viruses Reveals Cross-Family Horizontal Gene Transfer And Evolution Of Diverse Viral Lineages, Huiquan Liu, Yanping Fu, Jiatao Xie, Jiasen Cheng, Said A. Ghabrial, Guoqing Li, Youliang Peng, Xianhong Yi, Daohong Jiang

Plant Pathology Faculty Publications

BACKGROUND: Double-stranded (ds) RNA fungal viruses are typically isometric single-shelled particles that are classified into three families, Totiviridae, Partitiviridae and Chrysoviridae, the members of which possess monopartite, bipartite and quadripartite genomes, respectively. Recent findings revealed that mycovirus-related dsRNA viruses are more diverse than previously recognized. Although an increasing number of viral complete genomic sequences have become available, the evolution of these diverse dsRNA viruses remains to be clarified. This is particularly so since there is little evidence for horizontal gene transfer (HGT) among dsRNA viruses.

RESULTS: In this study, we report the molecular properties of two novel dsRNA mycoviruses that …

Sesquiterpene Synthase Gene And Protein, Joe Chappell, Bryan Greenhagen

Plant and Soil Sciences Faculty Patents

The invention relates to sesquiterpene synthases and methods for their production and use. Particularly, the invention provides nucleic acids comprising the nucleotide sequence of citrus valencene synthase (CVS) which codes for at least one CVS. The invention further provides nucleic acids comprising the nucleotide sequence coding for amino acid residues forming the tier 1 and tier 2 domains of CVS. The invention also provides for methods of making and using the nucleic acids and amino acids of the current invention.

Forage News [2012-06], Department Of Plant And Soil Sciences, University Of Kentucky

Forage News

• Advanced Grazing School
• Measuring the Moisture Content of Forage Using a Microwave Oven
• Thin Alfalfa Plantings: Deciding when to give up
• AFGC Forage Tour
• Kentucky Beef Cattle Market Update
• Thought for the Day

Identification Of Late Embryogenesis Abundant (Lea) Protein Putative Interactors Using Phage Display, Rekha Kushwaha, Taylor D. Lloyd, Kim R. Schäfermeyer, Santosh Kumar, A. Bruce Downie

Horticulture Faculty Publications

Arabidopsis thaliana seeds without functional SEED MATURATION PROTEIN1 (SMP1), a boiling soluble protein predicted to be of intrinsic disorder, presumed to be a LATE EMBRYOGENESIS ABUNDANT (LEA) family protein based on sequence homology, do not enter secondary dormancy after 3 days at 40 °C. We hypothesized that SMP1 may protect a heat labile protein involved in the promotion of secondary dormancy. Recombinant SMP1 and GmPM28, its soybean (Glycine max), LEA4 homologue, protected the labile GLUCOSE-6-PHOSPHATE DEHYROGENASE enzyme from heat stress, as did a known protectant, Bovine Serum Albumin, whether the LEA protein was in solution or attached to the bottom …

Forage News [2012-05], Department Of Plant And Soil Sciences, University Of Kentucky

Forage News

• 4th Annual Equine Farm & Facilities Expo
• Grazing School
• In Search of 300-Day Grazing
• Farmland Prices Expected to Keep Rising
• Record Prices, Low Numbers: Where Are We?
• Harvesting Summer Annual Grasses for Hay