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Beta-Carbonic Anhydrase Gene Expression Levels Change Depending On The Drought Severity In Both The Leaves And Roots Of Arabidopsis Thaliana, Sertan Çevi̇k, Oğuzhan Kurt, Ayşi̇n Güzel Değer, Ferit Kocaçinar, Rıza Bi̇nzet
Beta-Carbonic Anhydrase Gene Expression Levels Change Depending On The Drought Severity In Both The Leaves And Roots Of Arabidopsis Thaliana, Sertan Çevi̇k, Oğuzhan Kurt, Ayşi̇n Güzel Değer, Ferit Kocaçinar, Rıza Bi̇nzet
Turkish Journal of Botany
In this study, it was aimed to determine the relationship between β carbonic anhydrase (βCA) gene expression levels and drought stress severity. For this purpose, the expression levels of six βCA genes in the roots and leaves of Arabidopsis thaliana were investigated under mild, moderate, and severe drought conditions. In addition, changes in the biomass, leaf water content, photosynthetic efficiency, CA enzyme activity, free proline content, and lipid peroxidation were also determined. The results showed that all of the β CA gene expression levels and CA enzyme activity increased in the leaf under mild drought conditions; however, the expression levels …
Genome-Wide Identification Of Qtls Associated With Iron Deficiency Tolerance In Soybean Reveals Gata12 As The Integrator Of Iron Signaling In Roots, Amir Maqbool, Ahmet Şahi̇n, Allah Bakhsh, Emre Aksoy
Genome-Wide Identification Of Qtls Associated With Iron Deficiency Tolerance In Soybean Reveals Gata12 As The Integrator Of Iron Signaling In Roots, Amir Maqbool, Ahmet Şahi̇n, Allah Bakhsh, Emre Aksoy
Turkish Journal of Botany
Iron (Fe) is an essential micronutrient required for plant growth and development. However, its deficiency causes substantial yield losses, particularly in alkaline soils. Soybean (Glycine max) serves as an ideal model to study Fe deficiency chlorosis (IDC) due to its inefficient Fe uptake from the soil. Although the quantitative trait loci (QTL) associated with IDC tolerance were determined in soybean, the specific genes within these QTL regions remain unidentified. In this study, it was aimed to identify and analyze the expression levels of genes present in IDC-responsive soybean QTL under Fe deficiency. Through this investigation, 6593 genes were identified within …
Boron Toxicity Induces Sulfate Transporters At Transcriptional Level In Arabidopsis Thaliana, Ceyhun Kayihan, Emre Aksoy, Su Naz Mutlu
Boron Toxicity Induces Sulfate Transporters At Transcriptional Level In Arabidopsis Thaliana, Ceyhun Kayihan, Emre Aksoy, Su Naz Mutlu
Turkish Journal of Botany
Plants activate glutathione (GSH)-dependent detoxification pathways at biochemical and molecular levels under boron (B) toxicity. Sulfate uptake and transport are necessary for GSH biosynthesis in plants. Therefore, the transcriptional regulation of some sulfate transporters was determined in this study to clarify the importance of these transporters in leaf and root tissues of Arabidopsis thaliana under toxic B conditions. The expression level of SULTR1;3 was dramatically increased in leaf and root tissues under moderate and severe toxic B conditions, suggesting source-to-sink sulfate translocation under B toxicity. Stable expression levels of SULTR2;1, SULTR2;2, and low SULTR3;5 expression might restrict the sulfate movement …
Identification And Characterization Of A Long Fruit Mutant In Cucumis Sativus L., Yajing Hu, Xiaojing Dong, Yana Zhang, Rui Tang, Jiali Li, Chenxing Cao, Chunhua Chen, Zhonghai Ren, Lina Wang
Identification And Characterization Of A Long Fruit Mutant In Cucumis Sativus L., Yajing Hu, Xiaojing Dong, Yana Zhang, Rui Tang, Jiali Li, Chenxing Cao, Chunhua Chen, Zhonghai Ren, Lina Wang
Turkish Journal of Botany
A long fruit (lf) mutant was obtained from the cucumber inbred line 'SN5' by ethyl methanesulfonate (EMS)-mediated mutagenesis. We found that plant height and internode length of lf were significantly decreased, while leaf area was significantly increased in comparison to wild type (WT) plants. Higher carbohydrate level, together with lower chlorophyll content and photosynthetic parameters, was revealed in lf leaves than those of WT. Longer fruits, as well as enhanced fruit quality, were observed for mutant plants in comparison to WT, and the difference in fruit length was continuously increased with the development of fruits. ELISA and real-time PCR analyses …
Synergism And Antagonism In Plant Acclimation To Abiotic Stress Combinations, Karl-Josef Dietz
Synergism And Antagonism In Plant Acclimation To Abiotic Stress Combinations, Karl-Josef Dietz
Turkish Journal of Botany
Acclimation of plants to their environment involves mechanisms at all molecular levels and only the complete temporal and spatial pattern adequately describes the acclimation state. The diversity of physiological states realized in plants increases by the essentially infinite combinations of abiotic and biotic stresses, considering duration, impact order, and strength. The interference between stressors involves phenomena of synergism, antagonism, and indifference. This review exemplarily sketches principles of interference considering posttranslational modifications, the formation of molecular assemblies in regulosomes, the regulation of gene expression, and the vast number of other posttranscriptional controls e.g., in RNA processing and recruitment to ribosomes. Small …
Effect Of Fungicide Pretreatment On Lipid Peroxidation, Antioxidant Enzyme Systems And Proline Accumulation In Tomato (Lycopersicon Esculentum Mill.) Leaves Under High Temperature Stress, Eli̇f Yüzbaşioğlu
Turkish Journal of Botany
The aim of the present study was to determine the effect of high temperature stress (HT) and to evaluate the protective role of fungicide pretreatment against HT stress in tomato leaves. The oxidative stress was triggered under HT stress through an increase in the content of malondialdehyde (MDA) and hydrogen peroxide (H2O2). Also, it caused a decline in photosynthetic pigments and enhanced the proline in tomato. However, the activities of catalase (CAT; EC 1.11.1.6), peroxidase (POX; EC 1.11.1.7), and glutathione S-transferase (GST; EC.2.5.1.18), as well as ascorbate-glutathione cycle enzymes (APX, EC 1.11.1.11, DHAR, EC 1.8.5.1, MDHAR EC 1.6.5.4, GR; EC …
Dehydrins: An Overview Of Current Approaches And Advancement, Nesli̇han Saruhan Güler, Rabi̇ye Terzi̇
Dehydrins: An Overview Of Current Approaches And Advancement, Nesli̇han Saruhan Güler, Rabi̇ye Terzi̇
Turkish Journal of Botany
Since plants are exposed to diverse environmental stresses in their natural area, numerous reviews have demonstrated many aspects of dehydrins (DHNs), including structural and functional dynamics, and multiple roles such as membrane protection, cryoprotection of enzymes, chaperone feature, and protection from reactive oxygen species. In this review, we have focused on new information, and other promising and emerging topics of DHNs in plants. This review outlines particularly the potential regulatory mechanisms of DHNs associated with stress tolerance and the role of DHNs in morphological responses of the plants exposed to environmental stresses. Besides the discussion of the signaling pathways involved …
Characterization And Tissue-Specific As Well As Heat-Stress Expression Analysis Of Cbl-Interacting Protein Kinase Genes In Dimocarpus Longan Lour, Wei Zheng, Xueming Dong, Xuefei Yu, Qiuying Zhang
Characterization And Tissue-Specific As Well As Heat-Stress Expression Analysis Of Cbl-Interacting Protein Kinase Genes In Dimocarpus Longan Lour, Wei Zheng, Xueming Dong, Xuefei Yu, Qiuying Zhang
Turkish Journal of Botany
The CBL-interacting protein kinase (CIPK) gene family plays important roles in plant growth, development, and responses to abiotic stresses. To date, the CIPKs have been extensively identified and characterized in various plants. However, there have been few studies on CIPKs in Dimocarpus longan Lour (D. longan). In this study we identified eight putative CIPKs in D. longan (DlCIPKs) according to RNA-seq data. The physicochemical properties, subcellular localization, conserved motifs, phylogenetic relationships, glycosylation and phosphorylation sites, and gene ontology annotation were analyzed using bioinformatics tools. The tissue-specific and heat-stress-responsive expressions were systematically investigated as well. Ten motifs widely maintained by DlCIPKs …
Regulation Of Boron Toxicity Responses Via Glutathione-Dependent Detoxification Pathways At Biochemical And Molecular Levels In Arabidopsis Thaliana, Doğa Seli̇n Kayihan, Ceyhun Kayihan, Yelda Özden Çi̇ftçi̇
Regulation Of Boron Toxicity Responses Via Glutathione-Dependent Detoxification Pathways At Biochemical And Molecular Levels In Arabidopsis Thaliana, Doğa Seli̇n Kayihan, Ceyhun Kayihan, Yelda Özden Çi̇ftçi̇
Turkish Journal of Botany
The fine-tuned regulation of the Halliwell-Asada cycle (ascorbate-glutathione pathway) in Arabidopsis thaliana under boron (B) toxicity was shown in our previous report. In this study, we investigated the expression levels of some members of the glutathione S-transferase (GST) superfamily, such as phi (GSTF2, GSTF6, GSTF7, and GSTF8), tau (GSTU19), and zeta (GSTZ1) classes in Arabidopsis thaliana that were exposed to 1 mM boric acid (1B) and 3 mM boric acid (3B). Additionally, the expression levels of genes for glutathione (GSH) and phytochelatin biosynthesis as well as miR169 and miR156 were evaluated in Arabidopsis thaliana exposed to 1B and 3B. Moreover, …
Variations In Ethylene Sensitivity Among Mungbean [Vigna Radiata (L.) Wilczek] Genotypes Exposed To Drought And Waterlogging Stresses, Susheel Kumar Raina, Nikhil Raskar, Lalitkumar Aher, Ajay Kumar Singh, Dhammaprakash Pandhari Wankhede, Jagadish Rane, Paramjit Singh Minhas
Variations In Ethylene Sensitivity Among Mungbean [Vigna Radiata (L.) Wilczek] Genotypes Exposed To Drought And Waterlogging Stresses, Susheel Kumar Raina, Nikhil Raskar, Lalitkumar Aher, Ajay Kumar Singh, Dhammaprakash Pandhari Wankhede, Jagadish Rane, Paramjit Singh Minhas
Turkish Journal of Botany
Ethylene is an important phytohormone that regulates several aspects of plant development including those crucial for drought tolerance. The main aim of the present work was to investigate variability in ethylene sensitivity of mungbeans to seed germination, waterlogging, and leaf senescence, and the underlying transcriptional regulation of ethylene biosynthetic/responsive genes. Significant variation was observed for silver-nitrate-mediated germination inhibition and flooding stress tolerance among the mungbean genotypes. Genotypes with differential ethylene sensitivity (IC-325817 and IC-325756) revealed variable leaf senescence rates upon exposure to exogenously supplied ethephon. Drought induced significant upregulation of VrACS7 and VrERF genes in the more sensitive genotype, implicating …
Molecular Cloning And Characterization Of Ipomoea Nil Metallothioneins, Agnieszka Mierek-Adamska, Zuzanna Znajewska, Anna Goc, Grazyna Dabrowska
Molecular Cloning And Characterization Of Ipomoea Nil Metallothioneins, Agnieszka Mierek-Adamska, Zuzanna Znajewska, Anna Goc, Grazyna Dabrowska
Turkish Journal of Botany
Metallothioneins are low-molecular-weight, cysteine-rich proteins able to bind a variety of heavy metal ions. They are involved in the maintenance of micronutrient homeostasis and detoxification of nonessential heavy metals. Plant metallothioneins are classified into four main types based on the number and arrangement of cysteine residues. Here we report on the isolation of novel complementary and genomic DNA from a model for a short-day plant, Ipomoea nil (Japanese morning glory), that encodes 66- or 76-amino acid residue proteins. In silico and phylogenetic analysis of putative amino acid sequences confirmed that these 66- and 76-amino acid residue proteins belong, respectively, to …
A Novel Phytoene Synthase Paralog From Halophytic Salicorniaconfers Salinity Tolerance In Plants, Nazia Rehman, Muhammad Ramzan Khan, Ghulam Muhammad Ali
A Novel Phytoene Synthase Paralog From Halophytic Salicorniaconfers Salinity Tolerance In Plants, Nazia Rehman, Muhammad Ramzan Khan, Ghulam Muhammad Ali
Turkish Journal of Botany
Salicornia seems to be a suitable euhalophyte for the study of salt stress resilience mechanism and exploitation of salinity-tolerant genes for crop yield improvement. In this study, we cloned a novel SePSY1 gene from an extremely salt-tolerant glycophyte, Salicornia europaea. The sequence analysis revealed that the newly isolated gene is a paralog of PSY, suggesting a gene duplication event in Salicornia. The newly isolated gene encodes a protein of 419 amino acids. The C-terminus containing a Trans-IPPS-H domain with catalytic and binding regions exhibits considerable conservation in various species, while the N-terminus remained divergent. The structural analysis suggested a catalytic …
Molecular Characterization Of Tocopherol Biosynthesisgenes From Olea Europaea (L.) Cv. Ayvalık, Fati̇h Sezer, Kemal Meli̇k Taşkin
Molecular Characterization Of Tocopherol Biosynthesisgenes From Olea Europaea (L.) Cv. Ayvalık, Fati̇h Sezer, Kemal Meli̇k Taşkin
Turkish Journal of Botany
Vitamin E is a group of compounds that includes metabolites known as tocopherols, which have high antioxidant activities. Tocopherols are synthesized in plants, and their beneficial effects on human health have been reported for diseases such as coronary heart disease and cancer. Here we report the full-length transcripts encoding vitamin E biosynthetic enzymes from fruit mesocarp tissues using the rapid amplification of cDNA ends (RACE) method for the first time in olives. We characterized the structure of the genes 4-hydroxyphenylpyruvate dioxygenase (PDS1), homogentisate phytyltransferase 1 (HPT1), vitamin E defective 3 (VTE3), tocopherol cyclase (VTE1), and gamma tocopherol methyltransferase (GTMT), which …
Preliminary Analysis On The Transcripts Involved In Resistance Responses To Eumusae Leaf Spot Disease Of Banana Caused By Mycosphaerella Eumusae, A Recent Add-On Of The Sigatoka Disease Complex, Asoor Santhanam Saravanakkumar, Subbaraya Uma, Raman Thangavelu, Suthanthiram Backiyrani, Marimuthu Somasundaram Saraswathi, Viswanathan Sriram
Preliminary Analysis On The Transcripts Involved In Resistance Responses To Eumusae Leaf Spot Disease Of Banana Caused By Mycosphaerella Eumusae, A Recent Add-On Of The Sigatoka Disease Complex, Asoor Santhanam Saravanakkumar, Subbaraya Uma, Raman Thangavelu, Suthanthiram Backiyrani, Marimuthu Somasundaram Saraswathi, Viswanathan Sriram
Turkish Journal of Botany
Eumusae leaf spot disease, caused by Mycosphaerella eumusae, is widely distributed in different banana growing countries in Asia and Africa, causing severe losses in yield and quality of banana. The aim of this study was to identify the differentially expressed defense responsive genes through subtractive suppression hybridization (SSH) in the Musa-M. eumusae interaction system. In this method, leaf samples collected at different intervals between 0 and 72 h postinoculation (hpi) of M. eumusae from resistant (Manoranjitham-AAA) and susceptible (Grand Naine-AAA) cultivars were subjected to cDNA-SSH library construction. From the SSH library, 832 clones having the insert were selected and sequenced. …
Application Of Data Analysis In Cold Stress: A Case Study Of Nicotiana Benthamiana, İbrahi̇m Koç, Zi̇hni̇ Onur Çalişkaner, Ertuğrul Fi̇li̇z
Application Of Data Analysis In Cold Stress: A Case Study Of Nicotiana Benthamiana, İbrahi̇m Koç, Zi̇hni̇ Onur Çalişkaner, Ertuğrul Fi̇li̇z
Turkish Journal of Botany
Cold stress is a major environmental factor in plant life cycles. Nicotiana benthamiana, which belongs to the family Solanaceae, is one of the most commonly used model species in plant-microbe interaction studies. In total, 5205 differentially expressed genes were identified under cold stress in N. Benthamiana. Of these, 5029 were upregulated and 176 were downregulated within four time periods (4 h, 12 h, 24 h, and 48 h). The common up- and downregulated genes were identified as 692 and 6, respectively. The functional annotations of these genes were studied and these common genes involved in protein, RNA, miscellaneous enzyme families, …
Effects Of Cobalt Nanoparticles On Artemisinin Production And Gene Expression In Artemisia Annua, Bita Ghasemi, Ramin Hosseini, Fatemeh Dehghan Nayeri
Effects Of Cobalt Nanoparticles On Artemisinin Production And Gene Expression In Artemisia Annua, Bita Ghasemi, Ramin Hosseini, Fatemeh Dehghan Nayeri
Turkish Journal of Botany
The low artemisinin content in Artemisia has caused this compound to be expensive among medicines. Several attempts have been made to increase its production by altering the expression of different genes. However, no approach has been cost-effective. In this study, the expression levels of SQS and DBR2 genes were quantified by qRT-PCR, and artemisinin content was measured by HPLC in Artemisia annua cell suspension culture under nano cobalt particles elicitation. For this purpose, nano cobalt particles were used in 0.25, 2.5, and 5 mg L-1 concentrations and samples were collected after 8, 24, 48, and 72 h. The highest artemisinin …
Effects Of Exogenous Methyl Jasmonate And 2-Isopentenyladenine On Artemisinin Production And Gene Expression In Artemisia Annua, Mahboobeh Zare Mehrjerdi, Mohammad Reza Bihamta, Mansoor Omidi, Mohammad Reza Naghavi, Hassan Soltanloo, Mojtaba Ranjbar
Effects Of Exogenous Methyl Jasmonate And 2-Isopentenyladenine On Artemisinin Production And Gene Expression In Artemisia Annua, Mahboobeh Zare Mehrjerdi, Mohammad Reza Bihamta, Mansoor Omidi, Mohammad Reza Naghavi, Hassan Soltanloo, Mojtaba Ranjbar
Turkish Journal of Botany
Artemisinin, produced in very low amounts in Artemisia annua L. plants, is one of the most effective drugs in treating malaria. In this study, the effect of exogenous applications of methyl jasmonate (MeJA) and 2-isopentenyladenine (2-iP) in artemisinin production and the main genes of its biosynthesis pathway within a 1-week time period were investigated. Both MeJA and 2-iP increased artemisinin content, but no correlation was found between gene expression and its content. In plants treated with 2-iP, despite increased gene expression following elicitor application, downregulation of gene expression was also observed, which might be attributed to the negative effects of …