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Articles 1 - 7 of 7
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Mutations In Pmr5 Result In Powdery Mildew Resistance And Altered Cell Wall Composition, John P. Vogel, Ted K. Raab, Chris R. Somerville, Shauna C. Somerville
Mutations In Pmr5 Result In Powdery Mildew Resistance And Altered Cell Wall Composition, John P. Vogel, Ted K. Raab, Chris R. Somerville, Shauna C. Somerville
Ted K. Raab
Powdery mildews and other obligate biotrophic pathogens are highly adapted to their hosts and often show limited host ranges. One facet of such host specialization is likely to be penetration of the host cell wall, a major barrier to infection. A mutation in the pmr5 gene rendered Arabidopsis resistant to the powdery mildew species Erysiphe cichoracearum and Erysiphe orontii, but not to the unrelated pathogens Pseudomonas syringae or Peronospora parasitica. PMR5 belongs to a large family of plant-specific genes of unknown function. pmr5-mediated resistance did not require signaling through either the salicylic acid or jasmonic acid/ ethylene defense pathways, suggesting …
Ecological And Agricultural Applications Of Synchrotron Ir Microscopy, Ted K. Raab, John P. Vogel
Ecological And Agricultural Applications Of Synchrotron Ir Microscopy, Ted K. Raab, John P. Vogel
Ted K. Raab
The diffraction-limited spot size of synchrotron-based IR microscopes provides cell-specific, spectrochemical imaging of cleared leaf, stem and root tissues of the model genetic organism Arabidopsis thaliana, and mutant plants created either by T-DNA insertional inactivation or chemical mutagenesis. Spectra in the wavelength region from 6 to 12 microns provide chemical and physical information on the cell wall polysaccharides of mutants lacking particular biosynthetic enzymes (‘‘Cellulose synthase-like’’ genes). In parallel experiments, synchrotron IR microscopy delineates the role of Arabidopsis cell wall enzymes as susceptibility factors to the fungus Erysiphe cichoracearum, a causative agent of powdery mildew disease. Three genes, pmr4, pmr5, …
Pmr6, A Pectate Lyase–Like Gene Required For Powdery Mildew Susceptibility In Arabidopsis, John P. Vogel, Ted K. Raab, Celine Schiff, Shauna C. Somerville
Pmr6, A Pectate Lyase–Like Gene Required For Powdery Mildew Susceptibility In Arabidopsis, John P. Vogel, Ted K. Raab, Celine Schiff, Shauna C. Somerville
Ted K. Raab
The plant genes required for the growth and reproduction of plant pathogens are largely unknown. In an effort to identify these genes, we isolated Arabidopsis mutants that do not support the normal growth of the powdery mildew pathogen Erysiphe cichoracearum. Here, we report on the cloning and characterization of one of these genes, PMR6. PMR6 encodes a pectate lyase-like protein with a novel C-terminal domain. Consistent with its predicted gene function, mutations in PMR6 alter the composition of the plant cell wall, as shown by Fourier transform infrared spectroscopy. pmr6-mediated resistance requires neither salicylic acid nor the ability to perceive …
Visualizing Rhizosphere Chemistry Of Legumes With Mid-Infrared Synchrotron Radiation, Ted K. Raab, Michael C. Martin
Visualizing Rhizosphere Chemistry Of Legumes With Mid-Infrared Synchrotron Radiation, Ted K. Raab, Michael C. Martin
Ted K. Raab
A bright synchrotron light source operated by the Lawrence Berkeley National Laboratory served as an external source for infrared (IR) microscopy of plant root microcosms. Mid-IR light from synchrotrons is 2-3 orders of magnitude brighter than conventional sources, providing contrast based on the chemical information in the reflected signal at a spatial resolution near the diffraction-limit of 3-10 microns. In an experiment using plant root microcosms fitted with zinc selenide IR-transmissive windows (50 mm x 20 mm x 1 mm), we describe chemical differences and similarities within the root zone of mung bean (Vigna radiata L.), grown with or without …
Toward An Ecosystem Approach To Remediation In The Great Basin, Ted K. Raab
Toward An Ecosystem Approach To Remediation In The Great Basin, Ted K. Raab
Ted K. Raab
We consider the web of interactions among geologic materials, soils, plants, and animals to ask, "If mining or other extractive energy technologies occur in desert regions, what do we need to know to return the land to productivity?" The Great Basin represents a formidable challenge in this regard, as winters in these cold deserts and seasonal lack of moisture during parts of the year severely constrain the growing season for vegetation. Due to the nature of current or proposed mining activities in this region, we have chosen to concentrate on two potential pollutants: the trace element selenium (Se) and nitrate …
Variation In Competitive Abilities Of Plants And Microbes For Specific Amino Acids, David A. Lipson, Ted K. Raab, Steven K. Schmidt, Russ K. Monson
Variation In Competitive Abilities Of Plants And Microbes For Specific Amino Acids, David A. Lipson, Ted K. Raab, Steven K. Schmidt, Russ K. Monson
Ted K. Raab
Microbes are assumed to possess strong competitive advantages over plants for uptake of nutrients from the soil. The finding that non-mycorrhizal plants can obtain a significant fraction of their N requirement from soil amino acids contradicts this assumption. The amino acid glycine (Gly) has been used as a model amino acid in many recent studies. Our preliminary studies showed that Gly was a poor substrate for microbial growth compared to other amino acids. We tested the hypothesis that the alpine sedge Kobresia myosuroides competes better for Gly than for other amino acids because of decreased microbial demand for this compound. …
Non-Mycorrhizal Uptake Of Amino Acids By Roots Of The Alpine Sedge Kobresia Myosuroides: Implications For The Alpine Nitrogen Cycle, Ted K. Raab, David A. Lipson, Russ K. Monson
Non-Mycorrhizal Uptake Of Amino Acids By Roots Of The Alpine Sedge Kobresia Myosuroides: Implications For The Alpine Nitrogen Cycle, Ted K. Raab, David A. Lipson, Russ K. Monson
Ted K. Raab
Non-mycorrhizal plants of the alpine sedge, Kobresia myosuroides , take up the amino acid glycine from nutrient solutions at greater rates than NO3- or NH4+. The amino acids glutamate and proline were also taken up at high rates. Total plant biomass was twice as high after 4 months of growth on glycine, compared to NH4NO3, with significant increases in both root and leaf biomass. By taking advantage of differences in the d13C signature of air in the growth chamber and the glycine used for growth, a two-member mixing model was used to estimate that a significant amount of the glycine …