Spiroplasma Leucomae Sp. Nov., Isolated In Poland From White Satin Moth (Leucoma Salicis L.) Larvae, Marietta A. Oduori, Jerzy J. Lipa, Gail E. Gasparich
Oct 2005
Spiroplasma Leucomae Sp. Nov., Isolated In Poland From White Satin Moth (Leucoma Salicis L.) Larvae, Marietta A. Oduori, Jerzy J. Lipa, Gail E. Gasparich
Gail Gasparich
Spiroplasma sp. strain SMAT, isolated in Poland from white satin moth larvae, Leucoma salicis L. (Lepidoptera: Lymantriidae), was serologically distinct from other Spiroplasma species, groups or subgroups. Dark-field microscopy of the cells revealed the classical helical shape and subsequent transmission electron microscopy revealed cells surrounded by only a single cell membrane (lacking a cell wall). Growth of strain SMAT occurred in M1D medium at 30 °C. Strain SMAT catabolized both glucose and arginine, but did not hydrolyse urea. The G+C content of the DNA was 24±1 mol% as determined by melting temperature analysis. Serological analysis revealed a very weak cross-reactivity …
Spiroplasma Atrichopogonis Sp. Nov., From A Ceratopogonid Biting Midge, R. Thomas Koerber, Gail E. Gasparich, Mark F. Frana, William L. Grogan
Dec 2004
Spiroplasma Atrichopogonis Sp. Nov., From A Ceratopogonid Biting Midge, R. Thomas Koerber, Gail E. Gasparich, Mark F. Frana, William L. Grogan
Gail Gasparich
Spiroplasma sp. strain GNAT3597T was isolated from the biting midge genus Atrichopogon (Diptera: Ceratopogonidae). It was serologically distinct from other Spiroplasma species, groups or subgroups. Dark-field microscopy of the cells revealed the classical helical shape and subsequent transmission electron microscopy revealed cells surrounded by only a cell membrane (i.e. lacking a cell wall). Growth of strain GNAT3597T occurred in M1D medium at 30 °C. Strain GNAT3597T catabolized both glucose and arginine, but did not hydrolyse urea. The DNA G+C content of strain GNAT3597T was 29±1 mol%. Only one strain, SMCAT (Spiroplasma mirum), is serologically related to strain GNAT3597T, although the …
The Genus Spiroplasma And Its Non-Helical Descendants: Phylogenetic Classification, Correlation With Phenotype And Roots Of The Mycoplasma Mycoides Clade, Gail E. Gasparich, Robert F. Whitcomb, Deborah Dodge, Frank E. French, John Glass, David L. Williamson
Jan 2004
The Genus Spiroplasma And Its Non-Helical Descendants: Phylogenetic Classification, Correlation With Phenotype And Roots Of The Mycoplasma Mycoides Clade, Gail E. Gasparich, Robert F. Whitcomb, Deborah Dodge, Frank E. French, John Glass, David L. Williamson
Gail Gasparich
The genus Spiroplasma (helical mollicutes: Bacteria: Firmicutes: Mollicutes: Entomoplasmatales: Spiroplasmataceae) is associated primarily with insects. The Mycoplasma mycoides cluster (sensu Weisburg et al. 1989 and Johansson and Pettersson 2002 ) is a group of mollicutes that includes the type species – Mycoplasma mycoides – of Mycoplasmatales, Mycoplasmataceae and Mycoplasma. This cluster, associated solely with ruminants, contains five other species and subspecies. Earlier phylogenetic reconstructions based on partial 16S rDNA sequences and a limited sample of Spiroplasma and Mycoplasma sequences suggested that the genus Mycoplasma was polyphyletic, as the M. mycoides cluster and the grouping that consisted of the hominis and …
Revised Group Classification Of The Genus Spiroplasma, David L. Williamson, Robert F. Whitcomb, Joseph G. Tully, Gail E. Gasparich, David L. Rose, Patricia Carle, Joseph M. Bové, Kevin J. Hackett, Jean R. Adams, Roberta B. Henegar, Meghnad Konai, Claude Chastel, Frank E. French
Dec 1997
Revised Group Classification Of The Genus Spiroplasma, David L. Williamson, Robert F. Whitcomb, Joseph G. Tully, Gail E. Gasparich, David L. Rose, Patricia Carle, Joseph M. Bové, Kevin J. Hackett, Jean R. Adams, Roberta B. Henegar, Meghnad Konai, Claude Chastel, Frank E. French
Gail Gasparich
Significant changes have been made in the systematics of the genus Spiroplasma (class Mollicutes) since it was expanded by revision in 1987 to include 23 groups and eight sub-groups. Since that time, two additional spiroplasmas have been assigned group numbers and species names. More recently, specific epithets have been assigned to nine previously designated groups and three sub-groups. Also, taxonomic descriptions and species names have been published for six previously ungrouped spiroplasmas. These six new organisms are: Spiroplasma alleghenense (strain PLHS-1T) (group XXVI), Spiroplasma lineolae (strain TALS-2T) (group XXVII), Spiroplasma platyhelix (strain PALS-1T) (group XXVIII), Spiroplasma montanense (strain HYOS-1T) (group …
Spiroplasma Chrysopicola Sp. Nov., Spiroplasma Gladiatoris Sp. Nov., Spiroplasma Helicoides Sp. Nov., And Spiroplasma Tabanidicola Sp. Nov., From Tabanid (Diptera: Tabanidae) Flies, Robert F. Whitcomb, Frank E. French, Joseph G. Tully, Gail E. Gasparich, David L. Rose, Patricia Carle, Joseph M. Bove, Roberta B. Henegar, Meghnad Konai, Kevin J. Hackett, Jean R. Adams, Truman B. Clark, David L. Williamson
Jun 1997
Spiroplasma Chrysopicola Sp. Nov., Spiroplasma Gladiatoris Sp. Nov., Spiroplasma Helicoides Sp. Nov., And Spiroplasma Tabanidicola Sp. Nov., From Tabanid (Diptera: Tabanidae) Flies, Robert F. Whitcomb, Frank E. French, Joseph G. Tully, Gail E. Gasparich, David L. Rose, Patricia Carle, Joseph M. Bove, Roberta B. Henegar, Meghnad Konai, Kevin J. Hackett, Jean R. Adams, Truman B. Clark, David L. Williamson
Gail Gasparich
Four spiroplasma strains, DF-1T, TG-1T, TABS-2T, and TAUS-1T, all of which were isolated from deerflies or horseflies (Diptera: Tabanidae), were serologically distinct from previously described spiroplasma species, groups, and subgroups. Strain DF-1Toriginated from a Maryland deerfly (Chrysops sp.); strain TG-1Twas isolated from a Maryland horsefly (Tabanus gladiator); strain TAUS-1Toriginated from a member of the Tabanus abdominalis-limbatinevris complex of horseflies collected in Maryland; and strain TABS-2Twas isolated from a horsefly (Tabanus abactor) collected in Oklahoma. Cells of all of the strains appeared to be helical and motile when they were examined by dark-field microscopy. Cells of strain DF-1Tgrowing in M1D medium …
The Spiroplasma Motility Inhibition Test, A New Method For Determining Intraspecific Variation Among Colorado Potato Beetle Spiroplasmas, Kevin J. Hackett, J. J. Lipa, G. E. Gasparich, D. E. Lynn, M. Konai, M. Camp, R. F. Whitcomb
Dec 1996
The Spiroplasma Motility Inhibition Test, A New Method For Determining Intraspecific Variation Among Colorado Potato Beetle Spiroplasmas, Kevin J. Hackett, J. J. Lipa, G. E. Gasparich, D. E. Lynn, M. Konai, M. Camp, R. F. Whitcomb
Gail Gasparich
The Colorado potato beetle, Leptinotarsa decemlineata, is a major holarctic pest of solanaceous crops. Presumably, this insect spread from Solanum species in central America to the Mexican plateau, and this was followed by multiple invasions of North America and Europe. Attempts are being made to control this beetle by using a genetically modified spiroplasma that occurs naturally in its gut. In the current study, spiroplasmas isolated from beetles collected in North America and Poland exhibited serologic (spiroplasma motility inhibition test) and genomic (restriction fragment length polymorphism) profiles that suggest that there were multiple spiroplasma introductions. Two serovars were identified; one …
Improved Cultivation Systems For Isolation Of The Colorado Potato Beetle Spiroplasma., Meghnad Konai, K. J. Hackett, D. L. Williamson, J. J. Lipa, J. D. Pollack, G. E. Gasparich, E. A. Clark, D. C. Vacek, R. F. Whitcomb
Aug 1996
Improved Cultivation Systems For Isolation Of The Colorado Potato Beetle Spiroplasma., Meghnad Konai, K. J. Hackett, D. L. Williamson, J. J. Lipa, J. D. Pollack, G. E. Gasparich, E. A. Clark, D. C. Vacek, R. F. Whitcomb
Gail Gasparich
In North America, the Colorado potato beetle, Leptinotarsa decemlineata, is often infected with the host-specific, gut-inhabiting Colorado potato beetle spiroplasma (CPBS). CPBS is apparently a commensal, but it may be useful in biocontrol if it can be transformed to express an insect-lethal gene. Difficulty in cultivating the organism, however, has hindered the development of a suitable transformation system. In this study, we eliminated the need for coculturing CPBS with insect cells. CPBS was reliably isolated with the BBL Anaerobic GasPak Jar system (low redox, enhanced CO(inf2)), which was easier to use and less expensive than insect cell coculture methods. A …
