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Searching For Alternative Hosts And Determining The Variation In The Internal Transcribed Spacer (Its) Region Of Phakopsora Pachyrhizi And The Implications For Currently Used Molecular Diagnostic Assays: Subtitle, Tomas Allen Rush
LSU Master's Theses
Phakopsora pachyrhizi, the causal agent of soybean rust (SBR), is a serious disease on soybeans.The objectives of this project were to identify additional alternative and possible overwintering hosts of the SBR pathogen and to validate the current detection assays for SBR. For the first objective, we attempted to identify naturalized Louisiana legume(s) that can serve as hosts and overwintering sites for P. pachyrhizi. It was theorized that New Iberia and Iberville Parishes in south Louisiana are usually the first locations of soybean infection in the spring. This suggests that there may be alternative leguminous hosts located in these parishes that …
Assessment Of Interactions Amoung Viruses Infecting Sweetpotato, Charalambos D. Kokkinos
Assessment Of Interactions Amoung Viruses Infecting Sweetpotato, Charalambos D. Kokkinos
LSU Doctoral Dissertations
Viral diseases, especially those caused by mixed infections, are among the economically most important diseases of sweetpotato. Real-time PCR assays were developed for the detection and quantification of the potyviruses Sweet potato feathery mottle virus (SPFMV), Sweet potato virus G (SPVG), Ipomoea vein mosaic virus (IVMV); the crinivirus Sweet potato chlorotic stunt virus (SPCSV), and the begomovirus Sweet potato leaf curl virus (SPLCV) directly from infected sweetpotato plants. Titers of SPFMV, IVMV, and SPVG were lower in singly-infected sweetpotato plants compared to singly-infected plants of the standard indicator host Brazilian morning-glory (Ipomoea setosa) and the standard propagation host I. nil …