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A Molecular Genetic Study On The Tofl/Tofr Quorum-Sensing System Of Burkholderia Glumae: The Major Pathogen That Causes Bacterial Panicle Blight Of Rice, Ruoxi Chen
LSU Master's Theses
Burkholderia glumae is the major causal agent of an economically important rice disease, bacterial panicle blight (BPB). The known virulence factors of B. glumae share the TofI/TofR quorum sensing system as their regulator. tofI and tofR genes encode the N-acyl homoserine lactone (AHL) synthase for the B. glumae quorum sensing signals, N-octanoyl homoserine lactone (C8-HSL) and N-hexanoyl homoserine lactone (C6-HSL), and the receptor for AHL, respectively. To better understand the relationship between quorum sensing and known virulence factors (toxoflavin, flagella and lipase), as well as, putative virulence factors (i.e. extracellular polysaccharide), mutagenetic and phenotypic analyses were applied to this study. …