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Dynamics Of The Sweetpotato Potyvirus Aphid Pathosystem In Louisiana, Everlyne Nafula Wosula
Dynamics Of The Sweetpotato Potyvirus Aphid Pathosystem In Louisiana, Everlyne Nafula Wosula
LSU Doctoral Dissertations
Sweetpotato potyviruses [Sweet potato feathery mottle virus (SPFMV), Sweet potato virus G (SPVG) and Sweet potato virus 2 (SPV2)] commonly infect sweetpotato and weedy morning glories in the USA. These viruses are transmitted in a non-persistent manner by various aphid species and cause up to 15% yield loss. Sweetpotato is vegetatively propagated, and in the USA growers are supplied with virus tested propagation material to minimize impact of viruses. However the rapid re-infection of these materials with viruses warranted further studies to determine factors that influence the epidemiology of these viruses. The objectives of this study were: (i) to determine …
Molecular Approaches To Detect And Control Cercospora Kikuchii In Soybeans, Ashok Kumar Chanda
Molecular Approaches To Detect And Control Cercospora Kikuchii In Soybeans, Ashok Kumar Chanda
LSU Doctoral Dissertations
Cercospora leaf blight (CLB) caused by Cercospora kikuchii, has become a troublesome disease in the southern United States. C. kikuchii produces a non-hostspecific phytotoxin and a pathogenicity factor known as cercosporin during infection of soybean. A quantitative real-time PCR assay was developed for detection and quantification of C. kikuchii. The sensitivity of detection is 1 pg of genomic DNA. The assay detected the presence of C. kikuchii in soybean leaves long before the appearance of disease symptoms. C. kikuchii DNA levels in soybean leaves increased slowly during early soybean development, followed by a quick increase at late reproductive stages. Results …