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Comparative Proteome And Qpcr Analysis Of The Sugarcane Reaction To Leaf Scald Caused By Xanthomonas Albilineans, Freddy Fernando Garces-Obando
Comparative Proteome And Qpcr Analysis Of The Sugarcane Reaction To Leaf Scald Caused By Xanthomonas Albilineans, Freddy Fernando Garces-Obando
LSU Doctoral Dissertations
Leaf scald is an important disease of sugarcane caused by Xanthomonas albilineans (Xa). Leaf scald is controlled by the development and planting of resistant cultivars. However, erratic symptom expression makes conventional screening for resistance difficult. In addition, the mechanisms of resistance to leaf scald are not completely understood. Real-time, quantitative polymerase-chain-reaction (qPCR) assays were developed utilizing SYBR Green for a highly sensitive detection method or a TaqMan probe to quantify Xa populations in infected plants. Xa populations detected by qPCR followed similar trends to disease severity ratings and vascular infection results for two resistant and two susceptible cultivars under greenhouse …
Simplicillium Lanosoniveum, A Mycoparasite Of Phakopsora Pachyrhizi And Its Use As A Biological Control Agent, Nicole A. Ward
Simplicillium Lanosoniveum, A Mycoparasite Of Phakopsora Pachyrhizi And Its Use As A Biological Control Agent, Nicole A. Ward
LSU Doctoral Dissertations
In 2007, a filamentous fungus was recovered from sori of soybean rust (SBR), caused by Phakopsora pachyrhizi, collected from Louisiana and Florida. This fungus was identified as Simplicillium lanosoniveum on the basis of ITS sequence data and morphological traits. Simplicillium lanosoniveum was found coiling within sori and around urediniospores and showed a trophic attraction to rust sori, extending from sorus to sorus. In co-inoculated soybean leaves, the fungus did not grow or establish on leaf surfaces until sori erupted. Similarly, S. lanosoniveum colonized within 3 days and sporulated within 4 days on leaves showing disease symptoms. In field studies, when …