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Dsrna As A Potential Tool For Cercospora Leaf Blight Management And Elucidation Of A Factors That Contribute To Late Disease Onset In The Reproductive Growth Stage Of Soybean, Marija Zivanovic
LSU Doctoral Dissertations
Cercospora leaf blight (CLB) of soybean (Glycine max L. Merr.), an important disease in Louisiana, is primarily caused by Cercospora cf. flagellaris. The pathogen produces cercosporin, a toxin necessary for fungal virulence. So far, there are few commercial cultivars of soybean with CLB resistance and few effective fungicides to manage this disease. Consequently, alternative management approaches are needed. With that in mind, the first objective was to develop a screening method to compare the effectiveness of bacterially-expressed double stranded (ds) RNAs corresponding to genes associated with cercosporin accumulation (Avr4, CTB1, CTB8, AY-1 and HN-2 …
The Roles Of Avr4 In Fungal Virulence,Cercosporin Biosynthesis And Its Potential Use In Host Induced Gene Silencing For Controlling Cercospora Leaf Blight Disease Of Soybeans, Josielle Santos Rezende
The Roles Of Avr4 In Fungal Virulence,Cercosporin Biosynthesis And Its Potential Use In Host Induced Gene Silencing For Controlling Cercospora Leaf Blight Disease Of Soybeans, Josielle Santos Rezende
LSU Doctoral Dissertations
The AVR4 effector, secreted by Cladosporium fulvum, has been demonstrated to be involved in pathogen virulence. Recent studies further demonstrated that Avr4 is highly conserved among several Cercospora species, indicating a potential important role of this gene in fungal virulence. Therefore, investigation to determine whether this fungal effector gene is present in Cercospora cf. flagellaris (previously known as C. kikuchii), the causal agent of soybean cercospora leaf blight (CLB) disease, and whether it plays any role in CLB disease development, is of great interest. In the present study, the Avr4 gene from C. cf. flagellaris was cloned and mutants lacking …
Sensitivity And Resistance Of Cercospora Kikuchii, Causal Agent Of Cercospora Leaf Blight And Purple Seed Stain Of Soybean, To Selected Fungicides, Paul Patrick Price, Iii
Sensitivity And Resistance Of Cercospora Kikuchii, Causal Agent Of Cercospora Leaf Blight And Purple Seed Stain Of Soybean, To Selected Fungicides, Paul Patrick Price, Iii
LSU Doctoral Dissertations
ABSTRACT Isolates of Cercospora kikuchii, the causal agent of Cercospora leaf blight (CLB) and purple seed stain (PSS), were used to determine baseline sensitivities to selected quinone outside inhibitor (QoI) and demethylation inhibitor (DMI) fungicides by conducting radial growth assays on fungicide-amended media. The effective concentration to inhibit 50% radial growth (EC50) for each isolate was calculated by linear interpolation of the dose-response relationship. All baseline distributions were non-normal with outliers towards the less sensitive ends of the spectra, and median EC50 values for azoxystrobin, pyraclostrobin, trifloxystrobin, flutriafol, propiconazole, and tetraconazole were 0.081, 0.013, 0.012, 0.273, 0.143, 1.47 µg/ml, respectively. …
Cercospora Leaf Blight Of Soybean: Pathogen Vegetative Compatibility Groups, Population Structure, And Host Resistance, Guohong Cai
LSU Doctoral Dissertations
Nitrogen nonutilizing mutants were used to assess vegetative compatibility of 58 isolates of Cercospora kikuchii, 55 of which were isolated from soybean plants in Louisiana. Only 16 of 56 self-compatible isolates were assigned to six multi-member vegetative compatibility groups (VCGs), 01-06, with two or three isolates in each VCG. The other 40 isolates were not vegetatively compatible with any isolates other than themselves. All six multi-member VCGs contained isolates from different soybean cultivars, and three included isolates from different locations. Only one VCG included isolates both from soybean leaves and from seeds, while two and three multi-member VCGs included isolates …