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Investigation Of Cytochrome P450 Monooxygenases In S. Homoeocarpa For Chlorothalonil Biotransformation, Robert Green Jul 2017

Investigation Of Cytochrome P450 Monooxygenases In S. Homoeocarpa For Chlorothalonil Biotransformation, Robert Green

Masters Theses

Sclerotinia homoeocarpa (F.T. Bennett) is one of the most economically important pathogens on high amenity cool-season turfgrasses where it causes dollar spot. Due to decades of over-reliance and repeated chemical treatments, S. homoeocarpa has developed resistance and insensitivity to multiple classes of fungicides. To understand the genetic mechanisms of fungicide resistance, the whole genomes of two strains with varying resistance levels to fungicides, were sequenced. In unpublished data (Sang et al.), a RNA-sequencing analysis revealed three CYP450s that were validated to play a functional role in S. homoeocarpa’s resistance against different fungicide classes. We also identified CYP450 metabolic action …


Development Of Cold Hardy, Large Patch Resistant Zoysiagrass Cultivars For The Transition Zone, Mingying Xiang, J. Fry, M. Kennelly Jul 2017

Development Of Cold Hardy, Large Patch Resistant Zoysiagrass Cultivars For The Transition Zone, Mingying Xiang, J. Fry, M. Kennelly

Kansas Agricultural Experiment Station Research Reports

AZ. japonicagenotype TAES 5645 which exhibited resistance to large patch in preliminary studies conducted by our collaborators at Texas A&M University was used as a breeding parent there and crossed with 22 cold hardy zoysiagrasses, resulting in 985 progeny. These progeny were evaluated for cold hardiness and agronomic traits (establishment rate, overall quality, spring greenup, leaf texture, and genetic color) in Manhattan, KS; West Lafayette, IN; and Dallas, TX; from 2012 to 2014. This report focuses on the Manhattan, KS, results. From this work, 60 progeny were identified for further evaluation in larger plots. In fall 2016, ‘Meyer’ …


Impact Of Zoysiagrass And Tall Fescue Seed Mixtures On Brown Patch Severity, Mingying Xiang, J. Fry, M. Kennelly Jul 2017

Impact Of Zoysiagrass And Tall Fescue Seed Mixtures On Brown Patch Severity, Mingying Xiang, J. Fry, M. Kennelly

Kansas Agricultural Experiment Station Research Reports

Earlier research has demonstrated that polystands of zoysiagrass and tall fescue can be established successfully, with the potential to provide a high-quality turfgrass stand with reduced inputs. Our objective was to determine whether mixing zoysiagrass with tall fescue will reduce brown patch severity while maintaining overall acceptable quality. Studies were established at the Rocky Ford Turfgrass Research Center in Manhattan, KS. In the split-plot design, natural infection byRhizoctonia solanior a fungicide-treated control was the whole-plot treatment factor and species (tall fescue monostand and the zoysiagrass/tall fescue mixture) were subplots. During July and August 2016, when hot, humid weather …