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Full-Text Articles in Entire DC Network
Signaling Via Camp In Fungi: Interconnections With Mitogen-Activated Protein Kinase Pathways, James Kronstad, Adriana De Maria, Deanna Funnell, R. David Laidlaw, Nancy Lee, Mário Moniz De Sá, Marilee Ramesh
Signaling Via Camp In Fungi: Interconnections With Mitogen-Activated Protein Kinase Pathways, James Kronstad, Adriana De Maria, Deanna Funnell, R. David Laidlaw, Nancy Lee, Mário Moniz De Sá, Marilee Ramesh
Department of Plant Pathology: Faculty Publications
The cAMP signal transduction pathway controls a wide variety of processes in fungi. For example, considerable progress has been made in describing the involvement of cAMP pathway components in the control of morphogenesis in Saccharomyces cerevisiae, Ustilago maydis, and Magnaporthe grisea. These morphological processes include the establishment of filamentous growth in S. cerevisiae and U. maydis, and the differentiation of an appressorial infection structure in M. grisea. The discovery that appressorium formation requires cAMP signaling provides an immediate connection to fungal virulence. This connection may have broader implications among fungal pathogens because recent work indicates …
The Pseudomonas Syringae Pv. Tomato Hrpw Protein Has Domains Similar To Harpins And Pectate Lyases And Can Elicit The Plant Hypersensitive Response And Bind To Pectate, Amy O. Charkowski, James R. Alfano, Gail Preston, Jing Yuan, Sheng Yang He, Alan Collmer
The Pseudomonas Syringae Pv. Tomato Hrpw Protein Has Domains Similar To Harpins And Pectate Lyases And Can Elicit The Plant Hypersensitive Response And Bind To Pectate, Amy O. Charkowski, James R. Alfano, Gail Preston, Jing Yuan, Sheng Yang He, Alan Collmer
Center for Plant Science Innovation: Faculty and Staff Publications
The host-specific plant pathogen Pseudomonas syringae elicits the hypersensitive response (HR) in nonhost plants and secretes the HrpZ harpin in culture via the Hrp (type III) secretion system. Previous genetic evidence suggested the existence of another harpin gene in the P. syringae genome. hrpW was found in a region adjacent to the hrp cluster in P. syringae pv. tomato DC3000. hrpW encodes a 42.9-kDa protein with domains resembling harpins and pectate lyases (Pels), respectively. HrpW has key properties of harpins. It is heat stable and glycine rich, lacks cysteine, is secreted by the Hrp system, and is able to elicit …
A Sensitive Elisa For Pythium Ultimum Using Polyclonal And Species-Specific Monoclonal Antibodies, G. Y. Yuen, J. Q. Xia, C. L. Sutula
A Sensitive Elisa For Pythium Ultimum Using Polyclonal And Species-Specific Monoclonal Antibodies, G. Y. Yuen, J. Q. Xia, C. L. Sutula
Department of Plant Pathology: Faculty Publications
A double-antibody sandwich indirect enzyme-linked immunosorbent assay (ELISA) was developed for the detection and quantification of Pythium ultimum. A polyclonal antibody produced to cell walls of P. ultimum was used as the capture antibody, while a P. ultimum-specific monoclonal antibody (MAb E5) was used for recognition of the fungus. In the ELISA, culture extracts of 7 isolates of P. ultimum exhibited strong positive reactions, whereas none of the 37 isolates of other Pythium spp. and fungal genera had positive reactions. P. ultimum was detected by ELISA in roots of bean, cabbage, and sugar beet seedlings grown in pathogen-infested …
Interactions Between The Structural Domains Of The Rna Replication Proteins Of Plant-Infecting Rna Viruses, Erin K. O'Reilly, Zhaohui Wang, Roy C. French, C. Cheng Kao
Interactions Between The Structural Domains Of The Rna Replication Proteins Of Plant-Infecting Rna Viruses, Erin K. O'Reilly, Zhaohui Wang, Roy C. French, C. Cheng Kao
Department of Plant Pathology: Faculty Publications
Brome mosaic virus (BMV), a positive-strand RNA virus, encodes two replication proteins: the 2a protein, which contains polymerase-like sequences, and the 1a protein, with N-terminal putative capping and C-terminal helicase-like sequences. These two proteins are part of a multisubunit complex which is necessary for viral RNA replication. We have previously shown that the yeast two-hybrid assay consistently duplicated results obtained from in vivo RNA replication assays and biochemical assays of protein-protein interaction, thus permitting the identification of additional interacting domains. We now map an interaction found to take place between two 1a proteins. Using previously characterized 1a mutants, a perfect …
Mutational Analysis Of Area, A Transcriptional Activator Mediating Nitrogen Metabolite Repression In Aspergillus Nidulans And A Member Of The “Streetwise” Gata Family Of Transcription Factors, Richard A. Wilson, Herbert N. Arst Jr.
Mutational Analysis Of Area, A Transcriptional Activator Mediating Nitrogen Metabolite Repression In Aspergillus Nidulans And A Member Of The “Streetwise” Gata Family Of Transcription Factors, Richard A. Wilson, Herbert N. Arst Jr.
Fungal Molecular Plant-Microbe Interactions
Details a mutational analysis of AREA, a transcriptional activator mediating nitrogen metabolite repression in Aspergillus nidulans and a member of the “streetwise” GATA family of transcription factors.
A Partial Host Range Of The High Plains Virus Of Corn And Wheat, Dallas L. Seifers, Tom L. Harvey, T. J. Martin, Stanley G. Jensen
A Partial Host Range Of The High Plains Virus Of Corn And Wheat, Dallas L. Seifers, Tom L. Harvey, T. J. Martin, Stanley G. Jensen
Department of Plant Pathology: Faculty Publications
Barley (Hordeum vulgare), cheat (Bromus secalinus), corn (Zea mays), oat (Avena sativa), rye (Secale cereale), and wheat (Triticum aestivum) were infected by a Kansas isolate of the High Plains virus (HPV) in greenhouse experiments, but several other grass species were not. Infection of a host was dependent upon wheat curl mite numbers. Although both green foxtail (Setaria viridis) and yellow foxtail (S. glauca) are found naturally infected by HPV, only yellow foxtail could be infected in greenhouse experiments. Field sampling (1994 to 1996) of …
Certification And The American Phytopathological Society, L. J. Stowell, J. Amador, O.W. Barnett, R. J. Cook, D. E. Mathre, A. K. Vidaver, S. A. Tolin
Certification And The American Phytopathological Society, L. J. Stowell, J. Amador, O.W. Barnett, R. J. Cook, D. E. Mathre, A. K. Vidaver, S. A. Tolin
Department of Plant Pathology: Faculty Publications
The profession of plant pathology strives to improve the health and safety of the public through improved plant health systems. This includes the benefits afforded by well-managed urban landscapes and abundant food and fiber. However, the proud glow of the profession of plant pathology has dimmed over the past 20 years to the point that some members of the American Phytopathological Society (APS), the principal professional society of plant pathologists in the United States, are not sure what a plant pathologist should know or what a plant pathologist is responsible for (Phytopathology News 30:162). Without a unified, positive direction for …
Phylogenetic Relationships Within The Family Potyviridae: Wheat Streak Mosaic Virus And Brome Streak Mosaic Virus Are Not Members Of The Genus Rymovirus, Drake C. Stenger, Jeffrey S. Hall, Il-Ryong Choi, Roy C. French
Phylogenetic Relationships Within The Family Potyviridae: Wheat Streak Mosaic Virus And Brome Streak Mosaic Virus Are Not Members Of The Genus Rymovirus, Drake C. Stenger, Jeffrey S. Hall, Il-Ryong Choi, Roy C. French
Department of Plant Pathology: Faculty Publications
The complete nucleotide sequence of wheat streak mosaic virus (WSMV) has been determined based on complementary DNA clones derived from the 9,384-nucleotide (nt) RNA of the virus. The genome of WSMV has a 130-nt 5leader and 149-nt 3-untranslated region and is polyadenylated at the 3end. WSMV RNA encodes a single polyprotein of 3,035 amino acid residues and has a deduced genome organization typical for a member of the family Potyviridae (5-P1/HC-Pro/P3/6K1/CI/6K2/VPg-NIa/NIb/CP-3). Because WSMV shares with ryegrass mosaic virus (RGMV) the biological property of transmission by eriophyid mites, WSMV has been assigned to the genus Rymovirus, of which RGMV is …
Expression Of Human Lactoferrin Cdna Confers Resistance To Ralstonia Solanacearum In Transgenic Tobacco Plants, Zhanyuan Zhang, Dermot P. Coyne, Anne K. Vidaver, Amitava Mitra
Expression Of Human Lactoferrin Cdna Confers Resistance To Ralstonia Solanacearum In Transgenic Tobacco Plants, Zhanyuan Zhang, Dermot P. Coyne, Anne K. Vidaver, Amitava Mitra
Department of Plant Pathology: Faculty Publications
A construct containing a human lactoferrin cDNA was used to transform tobacco (Nicotiana tabacum) using an Agrobacterium-mediated DNA-transfer system to express this human protein in transgenic plants. Transformants were analyzed by Southern, Northern, and Western blots to determine integration of the cDNA into the plant genome and lactoferrin gene expression levels. Most transgenic plants demonstrated significant delays of bacterial wilt symptoms when inoculated with the bacterial pathogen Ralstonia solanacearum. Quantification of the expressed lactoferrin protein by enzyme-linked immunosorbent assay in transgenic plants indicated a significant positive relationship between lactoferrin gene expression levels and levels of disease …
Physiologic Specialization Of Puccinia Recondita F. Sp. Tritici In Nebraska During 1995 And 1996, J. E. Watkins, S. S. Rutledge, P. Stephen Baenziger, W. Youngquist
Physiologic Specialization Of Puccinia Recondita F. Sp. Tritici In Nebraska During 1995 And 1996, J. E. Watkins, S. S. Rutledge, P. Stephen Baenziger, W. Youngquist
Department of Plant Pathology: Faculty Publications
Field samples of Puccinia recondita f. sp. tritici, collected from four wheat-growing regions in Nebraska in 1995 and from three in 1996, were characterized for virulence. Twenty virulence phenotypes were identified in 1995 and 18 in 1996. Virulence phenotypes MBR-10,18 (virulent on Lr genes, 1, 3, 3ka, 10, 11, 18, and 30) and MDR-10,18 (virulent on Lr genes 1, 3, 3ka, 10, 11, 18, 24, and 30) were the most prevalent, with each phenotype comprising 21.6% of the isolates characterized in 1995. Of the 1995 isolates, 24% were virulent on 10 or more host genes. No virulence to Lr16 and …
Polymerase Chain Reaction Detection And Phylogenetic Characterization Of An Agent Associated With Yellow Vine Disease Of Cucurbits, Francisco J. Avila, Benny D. Bruton, Jacqueline Fletcher, J. L. Sherwood, Sam D. Pair, Ulrich Melcher
Polymerase Chain Reaction Detection And Phylogenetic Characterization Of An Agent Associated With Yellow Vine Disease Of Cucurbits, Francisco J. Avila, Benny D. Bruton, Jacqueline Fletcher, J. L. Sherwood, Sam D. Pair, Ulrich Melcher
Department of Plant Pathology: Faculty Publications
Diagnosis of yellow vine disease (YVD) in cucurbits, an important disease in the south-central United States, relies on external symptom appearance, phloem discoloration, and the presence of bacterium-like organisms (BLOs) in phloem. Polymerase chain reaction (PCR) amplification of BLO nucleotide sequences was explored as a means to improve diagnostic techniques. PCR, using a primer pair based on sequences of the citrus-greening BLO, amplified a 0.15-kilobase (kb) fragment from the DNA of symptomatic plants, but not from that of asymptomatic plants. Its nucleotide sequence suggested that the DNA amplified was of prokaryotic origin. A primer pair, designed to amplify nonspecific prokaryotic …
Studies On The Biology Of Striga Aspera (Scrophulariaceae) In Nigeria, Emmanuel Izaka Aigbokhan
Studies On The Biology Of Striga Aspera (Scrophulariaceae) In Nigeria, Emmanuel Izaka Aigbokhan
Biological Sciences Theses & Dissertations
Studies on Striga aspera are few and little information exists on its relationship with the morphologically similar and much studied S. hermonthica. Both species are sympatric in West Africa and if they hybridize, may serve as virulent gene reservoirs for each other.
The primary focus of this study was on the biology of S. aspera in Nigeria. The research focussed on its distribution, phenology, cytology, hybridization, seed dormancy, viability and germination in relation to S. hermonthica. Related studies involving the hybrids include genetic and morphological variability, pathogenicity and the simultaneous dual infection of a maize plant by both species.
In …
Selection For Resistance And Tolerance To Oat Mosaic Virus And Oat Golden Stripe Virus In Hexaploid Oats, Scott L. Walker, Steven Leath, J. Paul Murphy, Steven A. Lommel
Selection For Resistance And Tolerance To Oat Mosaic Virus And Oat Golden Stripe Virus In Hexaploid Oats, Scott L. Walker, Steven Leath, J. Paul Murphy, Steven A. Lommel
Steven Leath
Clubroot, Rachel Lancaster, Caroline Donald, Ian Porter
Clubroot, Rachel Lancaster, Caroline Donald, Ian Porter
Journal of the Department of Agriculture, Western Australia, Series 4
Rachel Lancaster, Caroline Donald and Ian Palmer, outline some control measures for clubroot, one of the most serious diseases of crucifers world wide.
Characterization Of The Large (L) Rna Of Peanut Bud Necrosis Tospovirus, S. Gowda, Satyanarayana Tatineni, R. A. Naidu, A. Mushegian, W. O. Dawson, D. V. R. Reddy
Characterization Of The Large (L) Rna Of Peanut Bud Necrosis Tospovirus, S. Gowda, Satyanarayana Tatineni, R. A. Naidu, A. Mushegian, W. O. Dawson, D. V. R. Reddy
Department of Plant Pathology: Faculty Publications
The nucleocapsids purified from peanut plants systemically infected with peanut bud necrosis virus (PBNV), a member of the genus Tospovirus, contained both viral(v) and viral complementary(vc) sense L RNAs. Defective forms of L RNA containing ‘core polymerase region’ were observed. The full length L RNA of PBNV was sequenced using overlapping cDNA clones. The 8911 nucleotide L RNA contains a single open reading frame (ORF) in the vc strand, and encodes a protein of 330 kDa. At the 50 and 30 termini of the v sense RNA there were 247 and 32 nt untranslated regions, respectively, containing an 18 …
A Phylogenetic Analysis Of Heterorhabditis (Nemata: Rhabditidae) Based On Internal Transcribed Spacer 1 Dna Sequence Data, B. J. Adams, A. M. Burnell, Thomas O. Powers
A Phylogenetic Analysis Of Heterorhabditis (Nemata: Rhabditidae) Based On Internal Transcribed Spacer 1 Dna Sequence Data, B. J. Adams, A. M. Burnell, Thomas O. Powers
Department of Plant Pathology: Faculty Publications
Internal transcribed spacer 1 sequences were used to infer phylogenetic relationships among 8 of the 9 described species and one putative species of the entomopathogenic nematode genus Heterorhabditis. Sequences were aligned and optimized based on pairwise genetic distance and parsimony criteria and subjected to a variety of sequence alignment parameters. Phylogenetic trees were constructed with maximum parsimony, cladistic, distance, and maximum likelihood algorithms. Our results gave strong support for four pairs of sister species, while relationships between these pairs also were resolved but less well supported. The ITS1 region of the nuclear ribosomal repeat was a reliable source of …
Lindsay M. Black, 1907 To 1997, Myron K. Brakke, D. V. R. Reddy
Lindsay M. Black, 1907 To 1997, Myron K. Brakke, D. V. R. Reddy
Department of Plant Pathology: Faculty Publications
Plant pathology and virology lost a pioneer when Lindsay MacLeod Black died on 23 December 1997. We lost a friend and mentor. Black was noted for proving conclusively that a plant virus can multiply in both leafhopper vectors and plants; characterizing unstable, leafhopper-transmitted viruses; and developing insect tissue cultures for studying plant viruses.
Signaling Via Camp In Fungi: Interconnections With Mitogen-Activated Protein Kinase Pathways, James Kronstad, Adriana De Maria, Deanna L. Funnell, R. David Laidlaw, Nancy Lee, Mário Moniz De Sá, Marilee Ramesh
Signaling Via Camp In Fungi: Interconnections With Mitogen-Activated Protein Kinase Pathways, James Kronstad, Adriana De Maria, Deanna L. Funnell, R. David Laidlaw, Nancy Lee, Mário Moniz De Sá, Marilee Ramesh
Department of Plant Pathology: Faculty Publications
The cAMP signal transduction pathway controls a wide variety of processes in fungi. For example, considerable progress has been made in describing the involvement of cAMP pathway components in the control of morphogenesis in Saccharomyces cerevisiae, Ustilago maydis, and Magnaporthe grisea. These morphological processes include the establishment of filamentous growth in S. cerevisiae and U. maydis, and the differentiation of an appressorial infection structure in M. grisea. The discovery that appressorium formation requires cAMP signaling provides an immediate connection to fungal virulence. This connection may have broader implications among fungal pathogens because recent work indicates that …
Management Of Red Squirrel Feeding Damage To Lodgepole Pine By Stand Density Manipulation And Diversionary Food, Thomas P. Sullivan
Management Of Red Squirrel Feeding Damage To Lodgepole Pine By Stand Density Manipulation And Diversionary Food, Thomas P. Sullivan
Proceedings of the Eighteenth Vertebrate Pest Conference (1998)
The red squirrel (Tamiasciurus hudsonicus) feeds on the vascular tissues of sapling lodgepole pine (Pinus contorta) during spring periods in forests of interior British Columbia and Alberta, Canada. This damage may lead to mortality and reduced growth of crop trees in managed stands. Manipulation of stand density by pre-commercial thinning to densities < 1,000 stems/ha is an effective method to lower squirrel populations and feeding damage. Lowering stand density enhances the growth of crop trees, and understory herbs and shrubs as wildlife habitat, while protecting trees from squirrel feeding. This approach has been successful in several forest ecological zones. An alternative management tool is provision of diversionary food (sunflower seed) for those stands susceptible to feeding damage, and where stand thinning has already been completed. Diversionary food can be applied aerially and is very cost effective for protecting managed stands. These techniques may be used to maintain or even enhance species diversity of small mammal communities in those forest stands requiring protection.
Peanut Yellow Spot Virus Is A Member Of A New Serogroup Of Tospovirus Genus Based On Small (S) Rna Sequence And Organization, Satyanarayana Tatineni, S. Gowda, K. Lakshminarayana Reddy, S. E. Mitchell, W. O. Dawson, D. V. R. Reddy
Peanut Yellow Spot Virus Is A Member Of A New Serogroup Of Tospovirus Genus Based On Small (S) Rna Sequence And Organization, Satyanarayana Tatineni, S. Gowda, K. Lakshminarayana Reddy, S. E. Mitchell, W. O. Dawson, D. V. R. Reddy
Department of Plant Pathology: Faculty Publications
Peanut yellow spot virus (PYSV) represents a distinct tospovirus species based on serology and nucleic acid hybridization. The sequence of the S RNA was 2 970 nucleotides with 22 nucleotide long inverted repeats (with three mismatches) at the termini. The coding was ambisense with a long open reading frame (ORF) in each strand. The 5'-large ORF (1 440 nucleotides in the viral sense (v) strand) encoded a protein with a predicted size of 53.2 kDa that was identified as the nonstructural (NSs) protein based on 16–21% sequence identity and 42– 48% sequence similarity with other tospoviruses. A 3' ORF (741 …