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Characterization Of Heterorhabditis Isolates By Pcr Amplification Of Segments Of Mtdna And Rdna Genes, Susan A. Joyce, Ann M. Burnell, Thomas O. Powers

Department of Plant Pathology: Faculty Publications

Restriction digests of amplified DNA from the mitochondrial genome and the nuclear ribosomal internally transcribed spacer region have been evaluated as genetic markers for species groups in Heterorhabditis. Six RFLP profiles have been identified. These profiles supported groupings determined by cross-breeding studies and were in agreement with less definitive groupings based on other biochemical and molecular methods. Digestion patterns of both amplification products provided strong evidence for the recognition of species groups, which include Irish, NW European, tropical, and a H. bacteriophora complex. The H. bacteriophora complex could be further resolved into three genotypes represented by H. zealandica, …