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Viruses And Virus-Like Particles Of Eukaryotic Algae, James L. Van Etten, Leslie C. Lane, Russel H. Meints

Department of Plant Pathology: Faculty Publications

TABLE OF CONTENTS:
INTRODUCTION
CHARACTERISTICS OF CHLORELLA SPECIES
General Properties of Chlorella Species
Symbiotic Chlorella Species
Chlorella Strains NC64A, Nla, Pbi, and SAG-241-80
DISCOVERY OF CHLORELLA VIRUSES
Initial Observations
Development of a Culturable System for PBCV-1
PROPERTIES OF CHLORELLA VIRUSES
General Characteristics of PBCV-1
Composition of PBCV-1
PBCV-1 Genome
Serology of PBCV-1
Additional Chlorella Strain NC64A Viruses
Viruses Infecting Chlorella Strain Pbi
REPLICATION OF PBCV-1
Attachment of PBCV-1 to Chlorella Strain NC64A
Intracellular Site of PBCV-1 Replication
Effect of PBCV-1 Infection on Host DNA, RNA, and Protein Syntheses
TRANSCRIPTION OF PBCV-1
VIRUSES ENCODE DNA METHYLTRANSFERASES AND DNA SITE-SPECIFIC (RESTRICTION) ENDONUCLEASES …

Action Of Heptaminol Hydrochloride On Contractile Properties In Frog Isolated Twitch Muscle Fibre, Bruno Allard, Vincent Jacquemont, Fouad Lemtiri-Chlieh, Bernard Pourrias, Oger Rougier

fouad Lemtiri-Chlieh

1 Heptaminol stopped or delayed the progressive decline in tension which characterizes the phenomenon of fatigue in frog isolated twitch muscle fibre. 2 Heptaminol had no action on the sodium, potassium and calcium voltage-dependent ionic conductances. 3 The hypothesis of an action via an internal alkalinization was tested by comparison with the action of NH4Cl. Both substances increased the tension. 4 The action of hepataminol was suppressed in sodium-free (TRIS) solution or in the presence of amiloride while the action of NH4Cl was always observed. 5 These results could be explained by a stimulation of the Na/H antiport by heptaminol.

Regulation Of Biotechnology, Henry I. Miller, Robert H. Burris, Anne K. Vidaver

Department of Plant Pathology: Faculty Publications

Greg Simon (Letters, 3 May, p. 629) makes the case that only federal regulation of releases of agricultural products will address health and safety concerns. Such a view is hardly surprising, since he drafted the federal statute for the comprehensive regulation of field research with recombinant DNA-manipulated organisms. The basic assumptions of this statute were contrary to those contained in the reports of the National Academy of Sciences (NAS) (1) and the National Research Council (NRC) (2) and also to the existing policies of government research and regulatory agencies. The statute would have regulated only those organisms that were manipulated …

Shade Tree Decline And Related Problems, John Hartman, Mary L. Witt

Agriculture and Natural Resources Publications

Shade tree decline is a complex disease situation since the cause frequently cannot be traced to one single factor. Typically, one or more primary stresses cause the health of a tree to deteriorate, and then secondary pathogens and/or insects contribute to further decline. Determining causes of decline requires careful examination of the tree and growing site as well as knowledge of the tree's history. Even then, diagnosis may be difficult because the original stress may be obscure or no longer present. Trees most commonly affected in Kentucky are Norway and sugar maples, ash and oaks (particularly pin oaks).

Β-Ionone Derivatives As Antifungal Agent, Joseph Kuc, Steven D. Salt

Plant Pathology Faculty Patents

To read this abstract, please download this patent.

Proceedings Of The 18th Annual Meeting, Southern Soybean Disease Workers (March 20-21, 1991, Lexington, Kentucky): National Perspective On Soybeans, Donald E. Hershman, J. Allen Wrather, Patrick D. Colyer, Gary W. Lawrence, Glenn G. Hammes

Southern Soybean Disease Workers: Conference Proceedings

Contents

General session

Presidential address. DE Hershman

Keynote address. P O'Connell

Invited speakers

Major Soybean Diseases of the Midwest and Their Management. TS Abney

Major Soybean Diseases of the Southeast and Their Management. WF Moore

Disease Resistance in the Defense of Yield: A Commercial Breeders Perspective. HL Gabe

Graduate student papers

Effects of potassium fertilization and weed control on the severity.of anthracnose and pod and stem blight of soybeans in Louisiana. FG Barker, GT Berggren, and JP Snow

Inheritance of soybean SDS response in segregating F₅ derived lines. WJ Matthews, VN Njiti, PT Gibson, and ML Shenaut

Selection of …

Size Differences Among Root-Knot Nematodes On Resistant And Susceptible Alyceclover Genotypes, L. E. Powers, R. A. Dunn, R. Mcsorley

Department of Plant Pathology: Faculty Publications

The influence of plant resistance on the size of individual root-knot nematodes was determined in greenhouse experiments. Five genotypes of alyceclover were inoculated with second-stage juveniles of Meloidogyne incognita race 3 or M. arenaria race 1. Plants were harvested at selected intervals and stained for detection of the nematodes, which were dissected from the roots. Length, width, and sagittal-sectional area of each animal were measured using an image-analysis system, and areas of nematodes in all stages were compared at different times and across alyceclover lines. Nematodes feeding on roots of resistant lines were consistently smaller than those on susceptible plants, …

Evaluation Of Aposphaeria Amaranthi As A Bioherbicide For Pigweed (Amaranthus Spp.), A. S. Mintz, G. J. Weidmann

Journal of the Arkansas Academy of Science

Studies were conducted to determine the potential of the fungus, Aposphaeria amaranth!, as a bioherbicide for pigweeds (Amaranthus spp.). Experiments to establish the environmental parameters necessary for control of tumble pigweed (A. albus) demonstrated that an 8-hr dew period was sufficient for control of seedlings with four to six leaves, and that temperatures ranging from 20 to 28 C were conducive for disease development. Conidial concentrations as lowas 1x 10s conidia per ml also were sufficient for plant mortality. Host range tests demonstrated pathogenicity of A. amaranthi to several other species of Amaranthus, including biotypes resistant to triazine herbicides. Disease …

Maine Blueberry Advisory Committee Research Report, H Y. Forsythe Jr, Judith A. Collins, J Riley, David H. Lambert, David E. Yarborough, E A. Osgood, Frank A. Drummond, John M. Smagula, Delmont Emerson, Warren Hedstrom, Alfred A. Bushway, Mary Ellen Camire, Rodney J. Bushway, Susan A. Ismail, Therese M. Work, Linda J. Irvine, Craig J. Schroeder, Jasotha Kugabalasooriar, Richard J. Rowe, Gilles H. Lemieux, Rene Verrault, Paul E. Cappiello, James D. Leiby, Michele C. Marra, Willem Brutsaert, David Brooks, E R. Huff

Wild Blueberry Research Reports

The 1990 edition of the Maine Blueberry Advisory Committee Research Reports was prepared for the Maine Wild Blueberry Commission and the University of Maine Wild Blueberry Advisory Committee by researchers with the Maine Agricultural Experiment Station and Maine Cooperative Extension Service at the University of Maine, Orono. Projects in this report include:

1. Biology and action thresholds of secondary blueberry pests

2. Control of blueberry maggot

3. Control of secondary blueberry pests

4. Application of steam as a method of controlling secondary pest insects on lowbush blueberry: a feasibility study

5. Pollination of the lowbush blueberry by native bees

6. …

Comment On The Reinstatement Of Xanthomonas Citri (Ex Hasse 1915) Gabriel Et Al. 1989 And X. Phaseoli (Ex Smith 1897) Gabriel Et Al. 1989: Indication Of The Need For Minimal Standards For The Genus Xanthomonas, J. M. Young, J. F. Bradbury, L. Gardan, R. I. Gvozdyak, D. E. Stead, Y. Takikawa, A. K. Vidaver

Department of Plant Pathology: Faculty Publications

The proposals to reinstate Xanthomonas citri (ex Hasse 1915) Gabriel et al. 1989 and X. phaseoli (Smith 1897) Gabriel et al. 1989 are examined in terms of conventional criteria for describing new species. We suggest that the descriptions presented are insufficient in terms of modern practice for the purposes of formal classification in the genus Xanthomonas. To create guidelines for future reinstatements, the Judicial Commission of the International Committee on Systematic Bacteriology is requested to arrange for the preparation and promulgation of minimal standards for Xanthomonas. The pathovars proposed, X. campestris pv. aurantifolii Gabriel et al. 1989 …

Sterol Composition Of The Corn Root Lesion Nematode, Pratylenchus Agilis, And Corn Root Cultures, David J. Chitwood, William R. Lusby

Harold W. Manter Laboratory of Parasitology: Faculty and Staff Publications

Sterols from mixed stages of the com root lesion nematode, Pratylenchus agilis, and uninfected corn root cultures were analyzed by gas chromatography-mass spectrometry. Twenty-eight sterols were identified in P. agilis, including nine not previously detected in nematodes. The major sterols were 24-ethylcholest-22-enol, 24-ethylcholesta-5,22-dienol, 24-methy1cholestanol, 24-ethylcholestanol, isofucostanol, and 24-ethylcholesterol. The principal corn root sterols were 24-ethylcholesta-5,22-dienol, 24-methylcholesterol, 24-ethylcholesterol, isofucosterol, and cycloartenol. Therefore, the major metabolic transformation of sterols by P. agilis was saturation of the sterol nucleus. In addition, very small amounts of 4α-methylsterols were biosynthesized by P. agilis. The 4-methylation pathway is unique to nematodes and was …

Origin And Evolution Of Defective Interfering Rnas Of Tomato Bushy Stunt Virus, David A. Knorr, Thomas Jack Morris

School of Biological Sciences: Faculty Publications

Viruses with defective genomes have been identified in association with virtually every major family of viruses and have been widely utilized as tools for investigating virus functions in animal cell culture systems (Perrault, 1981). It is generally thought that defective interfering viruses (DIs) arise through deletion, rearrangement, or recombination of a competent viral genome. DIs tack the ability for independent existence relying on their parental helper viruses to supply factors required for replication, maturation, and/or encapsidation (Huang and Baltimore, 1977). The interference attributed to DIs is thought to result from competition with the helper virus for factors required in trans …