Digital Commons Network^™

Gaca, The Response Regulator Of A Two-Component System, Acts As A Master Regulator In Pseudomonas Syringae Pv. Tomato Dc3000 By Controlling Regulatory Rna, Transcriptional Activators, And Alternate Sigma Factors, Asita Chatterjee, Yaya Cui, Hailian Yang, Alan Collmer, James R. Alfano, Arun K. Chatterjee

Department of Plant Pathology: Faculty Publications

Concerted investigations of factors affecting host-pathogen interactions are now possible with the model plant Arabidopsis thaliana and its model pathogen Pseudomonas syringae pv. tomato DC3000, as their whole genome sequences have become available. As a prelude to analysis of the regulatory genes and their targets, we have focused on GacA, the response regulator of a two-component system. The DC3000 gene was cloned by testing for the reversal of phenotypes of an Erwinia GacA– mutant. A GacA– mutant of DC3000 constructed by marker exchange produces much-reduced levels of transcripts of three alternate sigma factors: HrpL, required for the production of effector …

Activation Of A Coi1-Dependent Pathway In Arabidopsis By Pseudomonas Syringae Type Iii Effectors And Coronatine, Ping He, Satya Chintamanani, Zhongying Chen, Lihuang Zhu, Barbara N. Kunkel, James R. Alfano, Xiaoyan Tang, Jian-Min Zhou

Center for Plant Science Innovation: Faculty and Staff Publications

Gram-negative bacteria use a variety of virulence factors including phytotoxins, exopolysaccharides, effectors secreted by the type III secretion system, and cell-wall-degrading enzymes to promote parasitism in plants. However, little is known about how these virulence factors alter plant celluar responses to promote disease. In this study, we show that virulent Pseudomonas syringae strains activate the transcription of an Arabidopsis ethylene response factor (ERF) gene, RAP2.6, in a coronatine insensitive 1 (COI1)-dependent manner. A highly sensitive RAP2.6 promoter-firely luciferase (RAP2.6-LUC) reporter line was developed to monitor activities of various bacterial virulence genes. Analyses of P. syringae pv. tomato DC3000 mutants indicated …

Integrated Weed Management Plan For The Lower Las Vegas Wash, Elizabeth Bickmore

Publications (WR)

The Las Vegas Wash (Wash) is the primary drainage for the metropolitan Las Vegas Valley, eventually finding its way into the Colorado River watershed system. Pursuant to the recommendations of the Water Quality Citizens Advisory Committee, the Las Vegas Wash Coordination Committee (LVWCC) was formed in 1998 to develop a comprehensive plan for the long-term stabilization and management of the Wash. Consisting of representatives from 28 government agencies, businesses, environmental groups and citizens, the LVWCC formulated the Las Vegas Wash Comprehensive Adaptive Management Plan (CAMP) and designated the Southern Nevada Water Authority (SNWA) as the lead agency for the implementation …

The Complete Genome Sequence Of The Arabidopsis And Tomato Pathogen Pseudomonas Syringae Pv. Tomato Dc3000, C. Robin Buell, Vinita Joardar, Magdalen Lindeberg, Jeremy Selengut, Ian T. Paulsen, Michelle L. Gwinn, Robert J. Dodson, Robert T. Deboy, A. Scott Durkin, James F. Kolonay, Ramana Radupu, Sean Daugherty, Lauren Brinkac, Maureen J. Beanan, Daniel H. Haft, William C. Nelson, Tanja Davidsen, Nikhat Zafar, Liwei Zhou, Jia Liu, Qiaoping Yuan, Hoda Khouri, Nadia Fedorova, Bao Tran, Daniel Russell, Kristi Berry, Teresa Utterback, Susan E. Van Aken, Tamara V. Feldblyum, Mark D'Ascenzo, Wen-Ling Deng, Adela R. Ramos, James R. Alfano, Samuel Cartinhour, Arn K. Chatterjee, Terrence P. Delaney, Sondra G. Lazarowitz, Gregory B. Martin, David J. Schneider, Xiaoyan Tang, Carol L. Bender, Owen White, Claire M. Fraser, Alan Collmer

Department of Plant Pathology: Faculty Publications

We report the complete genome sequence of the model bacterial pathogen Pseudomonas syringae pathovar tomato DC3000 (DC3000), which is pathogenic on tomato and Arabidopsis thaliana. The DC3000 genome (6.5 megabases) contains a circular chromosome and two plasmids, which collectively encode 5,763 ORFs. We identified 298 established and putative virulence genes, including several clusters of genes encoding 31 confirmed and 19 predicted type III secretion system effector proteins. Many of the virulence genes were members of paralogous families and also were proximal to mobile elements, which collectively comprise7%of the DC3000 genome. The bacterium possesses a large repertoire of transporters for …

Effects Of Modification Of The Transcription Initiation Site Context On Citrus Tristeza Virus Subgenomic Rna Synthesis, Maria A. Ayllon, Siddarame Gowda, Satyanarayana Tatineni, Alexander V. Karasev, Scott Adkins, Munir Mawassi, Jose Guerri, Pedro Moreno, William O. Dawson

Department of Plant Pathology: Faculty Publications

Citrus tristeza virus (CTV), a member of the Closteroviridae, has a positive-sense RNA genome of about 20 kb organized into 12 open reading frames (ORFs). The last 10 ORFs are expressed through 3'-coterminal subgenomic RNAs (sgRNAs) regulated in both amounts and timing. Additionally, relatively large amounts of complementary sgRNAs are produced. We have been unable to determine whether these sgRNAs are produced by internal promotion from the full-length template minus strand or by transcription from the minus-stranded sgRNAs. Understanding the regulation of 10 sgRNAs is a conceptual challenge. In analyzing commonalities of a replicase complex in producing so many …

Cutter Assembly For Microscope And Related Method, Richard R. Muse, Lisa J. Vaillancourt, William J. Young

Plant Pathology Faculty Patents

A low-profile cutter assembly for use on a microscope and related cutting method are provided. The cutter assembly includes a support subassembly having a mounting ring for receiving and engaging the objective of a microscope. A cutter subassembly carried by the support subassembly is displaceable between a home and a forward position in response to actuation. Upon reaching the forward position, additional actuation causes the front end of the cutter subassembly to pivot toward the stage, thereby placing a cutter adjacent to or in contact with the specimen. By selectively actuating the cutter subassembly, multiple cuts may be performed, as …

Use Of A 3 Cytoplasm To Reduce Risk Of Gene Flow Through Sorghum Pollen, Jeffrey F. Pedersen, D. B. Marx, Deanna L. Funnell

Department of Plant Pathology: Faculty Publications

A critical impediment to field testing and deployment of transgenic sorghum [Sorghum bicolor (L.) Moench] is the threat of gene flow to weedy relatives through pollen. A technique using sorghum with A₃ cytoplasmic male sterility to control transgene flow through pollen while using nontransgenic pollinators is described and an experiment was designed to evaluate the risk of viable pollen flow using A₃ hybrids under field conditions. Seed set under pollinating bags (an indicator of fertile pollen) was evaluated at the University of Nebraska Field Laboratory at Ithaca, NE, in 2001 and 2002 on selfed F₂ progeny …

Bcl-2 Family Members Inhibit Oxidative Stress-Induced Programmed Cell Death In Saccharomyces Cerevisiae, Shao-Rong Chen, David Dunigan, Martin Dickman

Department of Plant Pathology: Faculty Publications

Selected antiapoptotic genes were expressed in baker’s yeast (Saccharomyces cerevisiae) to evaluate cytoprotective effects during oxidative stress. When exposed to treatments resulting in the generation of reactive oxygen species (ROS), including H₂O₂, menadione, or heat shock, wild-type yeast died and exhibited apoptotic-like characteristics, consistent with previous studies. Yeast strains were generated expressing nematode ced-9, human bcl-2, or chicken bcl-xl genes. These transformants tolerated a range of oxidative stresses, did not display features associated with apoptosis, and remained viable under conditions that were lethal to wildtype yeast. Yeast strains expressing a mutant antiapoptotic gene (bcl-2 …

Induced Resistance As A Mechanism Of Biological Control By Lysobacter Enzymogenes Strain C3, Ozlem Kilic-Ekici, Gary Y. Yuen

Department of Plant Pathology: Faculty Publications

Induced resistance was found to be a mechanism for biological control of leaf spot, caused by Bipolaris sorokiniana, in tall fescue (Festuca arundinacea) using the bacterium Lysobacter enzymogenes strain C3. Resistance elicited by C3 suppressed germination of B. sorokiniana conidia on the phylloplane in addition to reducing the severity of leaf spot. The pathogen-inhibitory effect could be separated from antibiosis by using heat-inactivated cells of C3 that retained no antifungal activity. Application of live or heat-killed cells to tall fescue leaves resulted only in localized resistance confined to the treated leaf, whereas treatment of roots resulted in …

Evolution Of Wheat Streak Mosaic Virus: Dynamics Of Population Growth Within Plants May Explain Limited Variation, Roy C. French, Drake C. Stenger

Department of Plant Pathology: Faculty Publications

Like many other plant RNA viruses, Wheat streak mosaic virus (WSMV) sequence diversity within and among infected plants is low given the large number of virions produced. This may be explained by considering aspects of plant virus life history. Intracellular replication of RNA viruses is predominately linear, not exponential, which means that the rate at which mutations accumulate also is linear. Bottlenecks during systemic movement further limit diversity. Analysis of mixed infections with two WSMV isolates suggests that about four viral genomes participate in systemic invasion of each tiller. Low effective population size increases the role of stochastic processes on …

Crop Updates 2003 - -Katanning, Robert F. Park, Robert Loughman, Colin Wellings, Greg Shea, Jocelyn Ball, Natasha Littlewood, Lucy Creagh, Alex Douglas, David Stephens, Ross Brennan, Mike Bolland, Narelle Hill, Phil Michael, Francoise A. Berlandier, Christiaan Valentine, Roy Latta, M. Rodger Beermier, Keith Devenish, Blakely Paynter, Kevin Young, Robin Wilson, Ian Barclay, Robyn Mclean, Jenny Garlinge, Bill Lambe, Neil Venn, Peter Clarke, Frances Hoyle, Leanne Schulz, Judith Devenish

Crop Updates

This session covers sixteen papers from different authors

1. Breeding Cereals for Rust Resistance – are we losing the battle? Robert F. Park, University of Sydney

2. Stripe rust – where to now for the WA wheat industry? Robert Loughman, Department of Agriculture, Colin Wellings, University of Sydney, Greg Shea, Department of Agriculture

3. Oaten hay production, Jocelyn Ball, Natasha Littlewood and Lucy Creagh, Department of Agriculture

4. Don’t rely on ‘Spray and Pray’ Alex Douglas, Department of Agriculture

5. Seasonal outlook: What is in store for 2003, David Stephens, Department of Agriculture

6. No-till copper, phosphorus and zinc …

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Review Of Molecular Biology Of Fungal Development, Mycology Series, Volume 15, Marty Dickman

Department of Plant Pathology: Faculty Publications

Numerous publications, books, and textbooks are available that treat the subject of developmental biology. Far less is available detailing the fungi, an important group of organisms that have implications in a broad variety of contexts from medical to agricultural to food, and of course as model systems for “higher” eukaryotes. This nicely edited book by Osiewacz is an attempt to fill that gap. He has compiled an impressive list of authors, who represent leaders in their respective fields. This gives readers a detailed and current view of a broad array of fungal processes and lifestyles, ranging from saprophytes to pathogens …

Proceedings Of The 30th Annual Meeting, Southern Soybean Disease Workers (March 3, 2003, Little Rock, Arkansas), Patrick Fenn, Kathy S. Mclean, Boyd Padgett, Jason Bond, Steve R. Koenning

Southern Soybean Disease Workers: Conference Proceedings

Contents

Southern Soybean Disease Workers 2002 treasury report. James Bond

United States Soybean Disease Loss Estimate for 2002. Compiled by Stephen R. Koenning

Evaluation of Reduced Rates and Timing of Azoxystrobin on Frogeye Leaf Spot Cliff M Coker

Effect of Fungicides on Soybean Disease, Yield and Seed Quality. RW Schneider, JD Siebert, CA Jones, and JL Griffin

Effect of Fungicide Seed Treatment on Soybean Establishment. ML Rosso, C Boger, G Bates, C Rothrock, T Kirkpatrick, and J Rupe

Influence of Soybean Cyst Nematode on Sudden Death Syndrome Development in Field Microplots in Arkansas. SL Giammaria, CB Boger, and JC Rupe …

Crop Updates - 2003 Lupins, Mark Sweetingham, Bevan J. Buirchell, Geoff Thomas, Amelia Mclarty, Harmohinder Dhammu, Martin Harries, Wayne Parker, Terry Piper, David Nicholson, Jairo Palta, Neil C. Turner, Bob French, Kedar Adhikari, Katia Stefanova, Françoise Berlandier, Glen Riethmuller, Paul Blackwell, Geoff Fosbery, Bill Crabtree, Tracy Gilham, Laurie Wahlsten, Pierre Fievez, Mike Collins, John Holmes, Dirranie Kirby, Ken Adcock, Roger Jones, Rohan Prince, Stuart Johnson, Ramon Hall, Madeleine Ball, Sofia Sipas, David Petterson

Crop Updates

This session covers twenty one papers from different authors

LUPIN ISSUES AND R & D DIRECTIONS

1. Mark Sweetingham, Department of Agriculture

   ACKNOWLEDGEMENTS

   VARIETIES AND BREEDING

2. New lupin line for release – WALAN2141, Bevan J, Buirchell, Mark Sweetingham, Geoff Thomas, Amelia McLarty, Harmohinder Dhammu and CVT and Lupin Breeding teams, Department of Agriculture

3. Lupin variety trial, Martin Harries and Wayne Parker, Department of Agriculture

4. Herbicide tolerance of new lupins, Harmohinder S. Dhammu, Terry Piper and David Nicholson, Department of Agriculture

   YELLOW AND ALBUS LUPINS

5. Selection for high lupin yield under terminal drought, Jairo A. Palta^1&2, Neil …

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Crop Updates - 2003 Oilseeds, Peter Nelson, Graham Walton, Hasan Zaheer, Paul Carmody, J. A. Fortescue, D. W. Turner, Janet Wroth, Wallace Cowling, Anh-Van Pham, R. F. Brennan, Glen Riethmuller, Greg Hamilton, Jo Hawksley, C. M. Zaicou-Kunesch, D. Eksteen, Françoise A. Berlandier, Christiaan Valentine, Owain Edwards, Roger Jones, Brenda Coutts, Lisa Smith, Jenny Hawkes, Ravjit Khangura, Moin Salam, Art J. Diggle, Martin J. Barbetti, Kevin Walden

Crop Updates

This session covers fifteen papers from different authors

ACKNOWLEDGMENTS

VARIETIES

1. Large scale canola varietal evaluation in WA, Peter Nelson, Oilseeds WA

2. Performance of IT and TT canola varieties in the medium and high rainfall agzones of WA 2001-02, Graham Walton, Hasan Zaheer and Paul Carmody, Department of Agriculture

QUALITY

1. Reproductive biology, cotyledon development and oil accumulation in canola, J.A. Fortescue and D.W. Turner, School of Plant Biology, Faculty of Natural and Agricultural Sciences, The University of Western Australia

2. Plant and environmental factors affecting oil concentration in canola – a mini-review, D.W. Turner, School of Plant Biology, …

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Crop Updates - 2003 Pulses, M. Harries, R. French, I. Pritchard, R. Beermier, N. Poulish, S. White, M. Seymour, P. White, T. Pope, M. Blyth, L. Young, N. Abbas, J. Plummer, K. Siddique, D. Harris, T. Khan, K. Regan, G. Riethmuller, B. Macleod, J. Berger, N. Turner, F. Shan, H. Clarke, P. Smith, M. Baker, H. Dhammu, T. Piper, D. Nicholson, M. Materne, J. Howieson, R. Yates, S. Loss, Z. Rengel, B. Bowden, M. Bolland, J. Croser, A. Harrod, J. Galloway, C. Beeck, J. Wroth, W. Cowling, M. Salam, A. Diggle, R. Jones, B. Coutts, L. Smith, O. Byrne, D. Hardie, J. Ridsill-Smith, H. Sharma, K. Mann, S. Kelly, S. Sharma, H. Hunter, V. Vanstone

Crop Updates

This session covers fifty one papers from different authors

2002 PULSE INDUSTRY HIGHLIGHTS

CONTRIBUTORS

BACKGROUND

2002 REGIONAL ROUNDUP

1.Northern Agricultural Region, M. Harries, Department of Agriculture

2.Central agricultural Region, R. French and I. Pritchard, Department of Agriculture

3.Great Southern and Lakes, R. Beermier, N. Poulish and S. White, Department of Agriculture

4.Esperance Mallee, M. Seymour, Department of Agriculture

PULSE PRODUCTION ECONOMY AND GENETIC IMPROVEMENT

5.Faba Bean, P. White, Department of Agriculture

6.Germplasm evaluation, P. White, T. Pope, M. Harries and M. Seymour, Department of Agriculture

7.Row spacing and sowing rate, M. Seymour, Department …

Crop Updates 2003 - Cereals, Graham Crosbie, Robert Loughman, Collin Wellings, Greg Shea, Simon Mckirdy, Neil C. Turner, Brenda Shackley, Wal Anderson, Darshan Sharma, Mohammad Amjad, Steve Penny Jr, Melanie Kupsch, Anne Smith, Veronika Reck, Pam Burgess, Glenda Smith, Elizabeth Tierney, Peter Burges, Moin Salam, Megan Collins, Art Diggle, Blakely Paynter, Roslyn Jetter, Kevin Young, Jocelyn Ball, Natasha Littlewood, Lucy Anderton, Irene Waters, Tim Setter, Jeff Russell, Reg Lance, Chengdao Li, Sue Broughton, Michael Jones, Grace Zawko, Keith Gregg, Stephen Loss, Frank Ripper, Ryan Guthrie, Daniel Bell, Patrick Gethin, Narelle Hill, Laurence Caeslake, Vivien Vanstone, Sean Kelly, Helen Hunter, Christopher R. Newman

Crop Updates

This session covers twenty one papers from different authors:

PLENARY

1. Recognising and responding to new market opportunities in the grains industry, Graham Crosbie, Manager, Grain Products Research, Crop Breeding, Plant Industries, Department of Agriculture

2. Stripe rust – where to now for the WA wheat industry? Robert Loughman¹, Colin Wellings² and Greg Shea¹¹Department of Agriculture, ²University of Sydney Plant Breeding Institute, Cobbitty (on secondment from NSW Agriculture)

3. Benefits of a Grains Biosecurity Plan, Dr Simon McKirdy, Plant Health Australia, Mr Greg Shea, Department of Agriculture

4. Can we improve …

The Pseudomonas Syringae Type Iii-Secreted Protein Hopptod2 Possesses Protein Tyrosine Phosphatase Activity And Suppresses Programmed Cell Death In Plants, Avelina Espinosa, Ming Guo, Vincent C. Tam, Zheng Qing Fu, James R. Alfano

Center for Plant Science Innovation: Faculty and Staff Publications

The bacterial plant pathogen Pseudomonas syringae possesses a type III protein secretion system that delivers many virulence proteins into plant cells. A subset of these proteins (called Avr proteins) is recognized by the plant’s innate immune system and triggers defences. One defence-associated response is the hypersensitive response (HR), a programmed cell death (PCD) of plant tissue. We have previously identified HopPtoD2 as a type III secreted protein from P. s. pv. tomato DC3000. Sequence analysis revealed that an N-terminal domain shared homology with Avr- PphD and a C-terminal domain was similar to protein tyrosine phosphatases (PTPs). We demonstrated that purified …

Evaluation Of Resistance Screening Methods For Sclerotinia Stem Rot Of Soybean And Dry Bean, Linda S. Kull, Tri D. Vuong, Kris S. Powers, Kent M. Eskridge, James R. Steadman, Glen L. Hartman

Department of Plant Pathology: Faculty Publications

Three methods to identify levels of resistance to Sclerotinia sclerotiorum in soybean (Glycine max) and dry bean (Phaseolus vulgaris) were compared using multiple data analyses. The three methods were mycelial plug inoculations of cotyledons, cut stems, and detached leaves. Six S. sclerotiorum isolates of known relative aggressiveness were inoculated on each of three soybean and dry bean cultivars with varied response to S. sclerotiorum. For soybean, all three inoculation methods accurately identified isolate aggressiveness irrespective of cultivar, but identification of susceptible and partially resistant soybean cultivars was influenced by isolate. For dry bean, the cotyledon and cut …

Fungal Biology And Agriculture: Revisiting The Field, O. Yarden, D. J. Ebbole, S. Freeman, R. J. Rodriguez, M. B. Dickman

Department of Plant Pathology: Faculty Publications

Plant pathology has made significant progress over the years, a process that involved overcoming a variety of conceptual and technological hurdles. Descriptive mycology and the advent of chemical plant-disease management have been followed by biochemical and physiological studies of fungi and their hosts. The later establishment of biochemical genetics along with the introduction of DNA-mediated transformation have set the stage for dissection of gene function and advances in our understanding of fungal cell biology and plant–fungus interactions. Currently, with the advent of high-throughput technologies, we have the capacity to acquire vast data sets that have direct relevance to the numerous …

Unusual Life Style Of Giant Chlorella Viruses, James L. Van Etten

Department of Plant Pathology: Faculty Publications

Paramecium bursaria chlorella virus (PBCV-1) is the prototype of a family of large, icosahedral, plaque-forming, dsDNA viruses that replicate in certain unicellular, eukaryotic chlorella-like green algae. Its 330-kb genome contains ~373 protein-encoding genes and 11 tRNA genes. The predicted gene products of ~50% of these genes resemble proteins of known function, including many that are unexpected for a virus, e.g., ornithine decarboxylase, hyaluronan synthase, GDP-D-mannose 4,6 dehydratase, and a potassium ion channel protein. In addition to their large genome size, the chlorella viruses have other features that distinguish them from most viruses. These features include: (a) The viruses encode multiple …

Are Chlorella Viruses A Rich Source Of Ion Channel Genes?, Ming Kang, Anna Moroni, Sabrina Gazzarrini, James L. Van Etten

Department of Plant Pathology: Faculty Publications

Plaque-forming dsDNA (>330 kb) viruses that infect certain unicellular, eukaryotic chlorella-like green algae contain ~375 protein-encoding genes. These proteins include a 94 amino acid K+ channel protein, called Kcv, as well as two putative ligand-gated ion channels. The viruses also encode other proteins that could be involved in the assembly and/ or function of ion channels, including protein kinases and a phosphatase, polyamine biosynthetic enzymes and histamine decarboxylase.

Transcription Strategy In A Closterovirus: A Novel 5'-Proximal Controller Element Of Citrus Tristeza Virus Produces 5'- And 3'-Terminal Subgenomic Rnas And Differs From 3' Open Reading Frame Controller Elements, Siddarame Gowda, Maria A. Ayllon, Satyanarayana Tatineni, Moshe Bar-Joseph, William O. Dawson

Department of Plant Pathology: Faculty Publications

Citrus tristeza virus (CTV) produces more than thirty 3'- or 5'-terminal subgenomic RNAs (sgRNAs) that accumulate to various extents during replication in protoplasts and plants. Among the most unusual species are two abundant populations of small 5'-terminal sgRNAs of approximately 800 nucleotides (nt) termed low-molecular-weight tristeza (LMT1 and LMT2) RNAs. Remarkably, CTV replicons with all 10 3' genes deleted produce only the larger LMT1 RNAs. These 5'-terminal positive-sense sgRNAs do not have corresponding negative strands and were hypothesized to be produced by premature termination during plus-strand genomic RNA synthesis. We characterized a cis-acting element that controls the production …

A Protein Kinase From Colletotrichum Trifolii Is Induced By Plant Cutin And Is Required For Appressorium Formation, M. B. Dickman, Y.-S. Ha, Z. Yang, B. J. Adams, C. Huang

Department of Plant Pathology: Faculty Publications

When certain phytopathogenic fungi contact plant surfaces, specialized infection structures (appressoria) are produced that facilitate penetration of the plant external barrier; the cuticle. Recognition of this hydrophobic host surface must be sensed by the fungus, initiating the appropriate signaling pathway or pathways for pathogenic development. Using polymerase chain reaction and primers designed from mammalian protein kinase C sequences (PKC), we have isolated, cloned, and characterized a protein kinase from Colletotrichum trifolii, causal agent of alfalfa anthracnose. Though sequence analysis indicated conserved sequences in mammalian PKC genes, we were unable to induce activity of the fungal protein using known activators …