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Rhizosphere Microbiomes In A Historical Maize-Soybean Rotation System Respond To Host Species And Nitrogen Fertilization At The Genus And Subgenus Levels, Michael A. Meier, Martha G. Lopez-Guerrero, Ming Guo, Marty Schmer, Josh Herr, James Schnable, James R. Alfano, Jinliang Yang Jun 2021

Rhizosphere Microbiomes In A Historical Maize-Soybean Rotation System Respond To Host Species And Nitrogen Fertilization At The Genus And Subgenus Levels, Michael A. Meier, Martha G. Lopez-Guerrero, Ming Guo, Marty Schmer, Josh Herr, James Schnable, James R. Alfano, Jinliang Yang

Department of Agronomy and Horticulture: Faculty Publications

Root-associated microbes are key players in plant health, disease resistance, and nitrogen (N) use efficiency. It remains largely unclear how the interplay of biological and environmental factors affects rhizobiome dynamics in agricultural systems. In this study, we quantified the composition of rhizosphere and bulk soil microbial communities associated with maize (Zea mays L.) and soybean (Glycine max L.) in a long-term crop rotation study under conventional fertilization and low-N regimes. Over two growing seasons, we evaluated the effects of environmental conditions and several treatment factors on the abundance of rhizosphere- and soil-colonizing microbial taxa. Time of sampling, host plant species, …


Comparing A Mixed Model Approach To Traditional Stability Estimators For Mapping Genotype By Environment Interactions And Yield Stability In Soybean [Glycine Max (L.) Merr.], Mary M. Happ, George L. Graef, Haichuan Wang, Reka Howard, Luis Posadas, David L. Hyten Mar 2021

Comparing A Mixed Model Approach To Traditional Stability Estimators For Mapping Genotype By Environment Interactions And Yield Stability In Soybean [Glycine Max (L.) Merr.], Mary M. Happ, George L. Graef, Haichuan Wang, Reka Howard, Luis Posadas, David L. Hyten

Department of Agronomy and Horticulture: Faculty Publications

Identifying genetic loci associated with yield stability has helped plant breeders and geneticists begin to understand the role and influence of genotype by environment (GxE) interactions in soybean [Glycine max (L.) Merr.] productivity, as well as other crops. Quantifying a genotype’s range of performance across testing locations has been developed over decades with dozens of methodologies available. This includes directly modeling GxE interactions as part of an overall model for yield, as well as methods which generate overall yield “stability” values from multi-environment trial data. Correspondence between these methods as it pertains to the outcomes of genome wide association studies …


A Bumper Crop Of Snps In Soybean Through High-Density Genotyping-By-Sequencing (Hd-Gbs), Davoud Torkamaneh, Jerome Laroche, Brian Boyle, David L. Hyten, Fancois Belzile Jan 2021

A Bumper Crop Of Snps In Soybean Through High-Density Genotyping-By-Sequencing (Hd-Gbs), Davoud Torkamaneh, Jerome Laroche, Brian Boyle, David L. Hyten, Fancois Belzile

Department of Agronomy and Horticulture: Faculty Publications

No abstract provided.


Molecular-Assisted Breeding For Soybean With High Oleic/Low Linolenic Acid And Elevated Vitamin E In The Seed Oil, Katherine Hagely, Anji Reddy Konda, Jeong-Hwa Kim, Edgar B. Cahoon, Kristin Bilyeu Jan 2021

Molecular-Assisted Breeding For Soybean With High Oleic/Low Linolenic Acid And Elevated Vitamin E In The Seed Oil, Katherine Hagely, Anji Reddy Konda, Jeong-Hwa Kim, Edgar B. Cahoon, Kristin Bilyeu

Center for Plant Science Innovation: Faculty and Staff Publications

The uses of vegetable oils are determined by functional properties arising from their chemical composition. Soybean oil was previously used in margarines and baked foods after partial hydrogenation to achieve heat and oxidative stability. This process, however, generates trans fats that are now excluded from food use because of cardiovascular health risks. Also present in soybean oil are the anti-oxidant tocopherols, with α- tocopherol (vitamin E) typically present as a minor component compared to γ-tocopherol. Genetic improvement of the fatty acid profile and tocopherol profile is an attractive solution to increase the functional and health qualities of soybean oil. The …


Development Of Mping-Based Activation Tags For Crop Insertional Mutagenesis, Alexander Johnson, Edward Mcassey, Stephanie Diaz, Jacod Reagin, Priscilla S. Redd, Daymond R. Parrilla, Hanh Nguyen, Adrian Stec, Lauren A.L. Mcdaniel, Thomas E. Clemente, Robert M. Stupar, Wayne A. Parrott, C. Nathan Hancock Jan 2021

Development Of Mping-Based Activation Tags For Crop Insertional Mutagenesis, Alexander Johnson, Edward Mcassey, Stephanie Diaz, Jacod Reagin, Priscilla S. Redd, Daymond R. Parrilla, Hanh Nguyen, Adrian Stec, Lauren A.L. Mcdaniel, Thomas E. Clemente, Robert M. Stupar, Wayne A. Parrott, C. Nathan Hancock

Department of Agronomy and Horticulture: Faculty Publications

Modern plant breeding increasingly relies on genomic information to guide crop im- provement. Although some genes are characterized, additional tools are needed to effectively identify and characterize genes associated with crop traits. To address this need, the mPing element from rice was modified to serve as an activation tag to in- duce expression of nearby genes. Embedding promoter sequences in mPing resulted in a decrease in overall transposition rate; however, this effect was negated by using a hyperactive version of mPing called mmPing20. Transgenic soybean events carrying mPing-based activation tags and the appropriate transposase expression cassettes showed evidence of transposition. …