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Physiology

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Theses and Dissertations

2012

Angiogenesis

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The Effect Of Various Chemical Factors On Angiogenesis In The Chick Chorio-Allantoic Membrane, Heather Hammond Jun 2012

The Effect Of Various Chemical Factors On Angiogenesis In The Chick Chorio-Allantoic Membrane, Heather Hammond

Theses and Dissertations

The chick chorio-allantoic membrane (CAM) contains a complex vascular network commonly used to study angiogenesis. The application of chemical factors and oxygen barrier films onto this tissue can easily influence the process of angiogenesis. In this study, oxygen barrier film patches (Krehalon, polyvinylidene chloride, 12 μm thick, O2 transmission rate = 2.19 cm3•ml/100 in2•day•atm) were applied to areas of the CAM. Holes were made in the film and alginate beads incubated in various chemical factors were placed in the holes. After 24 and 48 hours of exposure to the alginate beads, images were taken of the tissue using a stereomicroscope …