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Localization Of Native Mms13 To The Magnetosome Chain Of Magnetospirillum Magneticum Amb-1 Using Immunogold Electron Microscopy, Immunofluorescence Microscopy And Biochemical Analysis, Zachery Oestreicher, Carmen Valverde-Tercedor, Eric Mumper, Lumarie Pérez-Guzmán, Nadia N. Casillas-Ituarte, Concepcion Jimenez-Lopez, Dennis A. Bazylinski, Steven K. Lower, Brian H. Lower
Localization Of Native Mms13 To The Magnetosome Chain Of Magnetospirillum Magneticum Amb-1 Using Immunogold Electron Microscopy, Immunofluorescence Microscopy And Biochemical Analysis, Zachery Oestreicher, Carmen Valverde-Tercedor, Eric Mumper, Lumarie Pérez-Guzmán, Nadia N. Casillas-Ituarte, Concepcion Jimenez-Lopez, Dennis A. Bazylinski, Steven K. Lower, Brian H. Lower
Life Sciences Faculty Research
Magnetotactic bacteria (MTB) biomineralize intracellular magnetite (Fe3O4 ) crystals surrounded by a magnetosome membrane (MM). The MM contains membrane-specific proteins that control Fe3O4 mineralization in MTB. Previous studies have demonstrated that Mms13 is a critical protein within the MM. Mms13 can be isolated from the MM fraction of Magnetospirillum magneticum AMB-1 and a Mms13 homolog, MamC, has been shown to control the size and shape of magnetite nanocrystals synthesized in-vitro. The objective of this study was to use several independent methods to definitively determine the localization of native Mms13 in M. magneticum AMB-1. Using Mms13-immunogold labeling and transmission electron microscopy …