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Integrated Magnetic Bionanocomposites Through Nanoparticle-Mediated Assembly Of Ferritin, S Srivastava, B Samanta, Bj Jordan, R Hong, Q Xiao, Mt Tuominen, Vm Rotello
Integrated Magnetic Bionanocomposites Through Nanoparticle-Mediated Assembly Of Ferritin, S Srivastava, B Samanta, Bj Jordan, R Hong, Q Xiao, Mt Tuominen, Vm Rotello
Vincent Rotello
Magnetic (FePt) and nonmagnetic (Au) nanoparticles were used to assemble ferritin into near-monodisperse bionanocomposites featuring regular interparticle spacing. The FePt/ferritin assemblies are integrated magnetic materials with ferritin providing added magnetic volume fraction to the magnetic nanocomposite. These assemblies differ from either of their constituent particles in terms of blocking temperature (TB), net magnetic moment, coercivity, and remnance.
Biomimetic Interactions Of Proteins With Functionalized Nanoparticies: A Thermodynamic Study, M De, Cc You, S Srivastava, Vm Rotello
Biomimetic Interactions Of Proteins With Functionalized Nanoparticies: A Thermodynamic Study, M De, Cc You, S Srivastava, Vm Rotello
Vincent Rotello
Gold nanoparticles (NPs) functionalized with L-amino acid-terminated monolayers provide an effective platform for the recognition of protein surfaces. Isothermal titration calorimetry (ITC) was used to quantify the binding thermodynamics of these functional NPs with alpha-chymotrypsin (ChT), histone, and cytochrome c (CytC). The enthalpy and entropy changes for the complex formation depend upon the nanoparticle structure and the surface characteristics of the proteins, e.g., distributions of charged and hydrophobic residues on the surface. Enthalpy-entropy compensation studies on these NP-protein systems indicate an excellent linear correlation between DeltaH and TDeltaS with a slope (alpha) of 1.07 and an intercept (TDeltaS0) of 35.2 …
Array-Based Sensing Of Proteins Using Conjugated Polymers, Or Miranda, Cc You, R Phillips, Ib Kim, Ps Ghosh, Uhf Bunz, Vm Rotello
Array-Based Sensing Of Proteins Using Conjugated Polymers, Or Miranda, Cc You, R Phillips, Ib Kim, Ps Ghosh, Uhf Bunz, Vm Rotello
Vincent Rotello
A sensor array comprising six functionalized poly(p-phenyleneethynylene) polymers was constructed and used to detect and identify protein analytes. The presence of proteins alters the fluorescence properties of the polymers, generating a distinct fluorescence response pattern for a given protein. Linear discriminant analyses accurately differentiate the patterns of 17 proteins at nano- to micromolar concentrations. An identification accuracy of 97% was obtained on the basis of the tests with 68 unknown protein samples from the training set.
Peptide Ligation Catalyzed By Functionalized Gold Nanoparticles, Y Fillon, A Verma, P Ghosh, D Ernenwein, Vm Rotello, J Chmielewski
Peptide Ligation Catalyzed By Functionalized Gold Nanoparticles, Y Fillon, A Verma, P Ghosh, D Ernenwein, Vm Rotello, J Chmielewski
Vincent Rotello
Trimethylammonium functionalized gold nanoparticles are demonstrated as templates for the assembly of peptide fragments and their subsequently promoted ligation. This system displays the use of organically tailored nanoparticles as effective supramolecular reagents for catalyzing bond-forming reactions and may also serve as a model for prebiotic conditions where charged surfaces may have promoted the polymerization of the early biopolymers.
Probing The Solvent-Induced Tautomerism Of A Redox-Active Ureidopyrimidinone, Am Alexander, M Bria, G Brunklaus, S Caldwell, G Cooke, Jf Garety, Sg Hewage, Y Hocquel, N Mcdonald, G Rabani, G Rosair, Bo Smith, Hw Spiess, Vm Rotello, P Woisel
Probing The Solvent-Induced Tautomerism Of A Redox-Active Ureidopyrimidinone, Am Alexander, M Bria, G Brunklaus, S Caldwell, G Cooke, Jf Garety, Sg Hewage, Y Hocquel, N Mcdonald, G Rabani, G Rosair, Bo Smith, Hw Spiess, Vm Rotello, P Woisel
Vincent Rotello
Trimethylammonium functionalized gold nanoparticles are demonstrated as templates for the assembly of peptide fragments and their subsequently promoted ligation. This system displays the use of organically tailored nanoparticles as effective supramolecular reagents for catalyzing bond-forming reactions and may also serve as a model for prebiotic conditions where charged surfaces may have promoted the polymerization of the early biopolymers.
Core-Like Particles Of An Enveloped Animal Virus Can Self-Assemble Efficiently On Artificial Templates, Nl Goicochea, M De, Vm Rotello, S Mukhopadhyay, B Dragnea
Core-Like Particles Of An Enveloped Animal Virus Can Self-Assemble Efficiently On Artificial Templates, Nl Goicochea, M De, Vm Rotello, S Mukhopadhyay, B Dragnea
Vincent Rotello
Alphaviruses are animal viruses holding great promise for biomedical applications as drug delivery vectors, functional imaging probes, and nanoparticle delivery vesicles because of their efficient in vitro self-assembly properties. However, due to their complex structure, with a protein capsid encapsulating the genome and an outer membrane composed of lipids and glycoproteins, the in-vitro self-assembly of virus-like particles, which have the functional virus coat but carry an artificial cargo, can be challenging. Fabrication of such alphavirus-like particles is likely to require a two-step process: first, the assembly of a capsid structure around an artificial core, second the addition of the membrane …
Binding And Templation Of Nanoparticle Receptors To Peptide Alpha-Helices Through Surface Recognition, P. S. Ghosh, A. Verma, Vincent M. Rotello
Binding And Templation Of Nanoparticle Receptors To Peptide Alpha-Helices Through Surface Recognition, P. S. Ghosh, A. Verma, Vincent M. Rotello
Vincent Rotello
Nanoparticles featuring highly flexible chains provide templateable surfaces for recognition of peptides.