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A Role For Huntingtin In Dynein/Dynactin-Mediated Vesicle Trafficking, Jennifer Ross, Juliane P. Caviston, Sheila M. Antony, Mariko Tokito, Erika L.F Holzbaur
A Role For Huntingtin In Dynein/Dynactin-Mediated Vesicle Trafficking, Jennifer Ross, Juliane P. Caviston, Sheila M. Antony, Mariko Tokito, Erika L.F Holzbaur
Jennifer Ross
Cytoplasmic dynein is a multisubunit microtubule motor complex that, together with its activator, dynactin, drives vesicular cargo toward the minus ends of microtubules. Huntingtin (Htt) is a vesicle-associated protein found in both neuronal and nonneuronal cells that is thought to be involved in vesicular transport. In this study, we demonstrate through yeast two-hybrid and affinity chromatography assays that Htt and dynein intermediate chain interact directly; endogenous Htt and dynein coimmunoprecipitate from mouse brain cytosol. Htt RNAi in HeLa cells results in Golgi disruption, similar to the effects of compromising dynein/dynactin function. In vitro studies reveal that Htt and dynein are …
Dynamic Reorganization Of Eg5 In The Mammalian Spindle Throughout Mitosis Requires Dynein And Tpx2, Jennifer Ross, C. Fagerstrom, G. Yang, E. S. Collins, N. Ma, N. P. Ferenz, S. Balchand, P. Wadsworth, J. Titus, M. Qiu, A. Gabel
Dynamic Reorganization Of Eg5 In The Mammalian Spindle Throughout Mitosis Requires Dynein And Tpx2, Jennifer Ross, C. Fagerstrom, G. Yang, E. S. Collins, N. Ma, N. P. Ferenz, S. Balchand, P. Wadsworth, J. Titus, M. Qiu, A. Gabel
Jennifer Ross
Kinesin-5 is an essential mitotic motor. However, how its spatial-temporal distribution is regulated in mitosis remains poorly understood. We expressed localization and affinity purification-tagged Eg5 from a mouse bacterial artificial chromosome (this construct was called mEg5) and found its distribution to be tightly regulated throughout mitosis. Fluorescence recovery after photobleaching analysis showed rapid Eg5 turnover throughout mitosis, which cannot be accounted for by microtubule turnover. Total internal reflection fluorescence microscopy and high-resolution, single-particle tracking revealed that mEg5 punctae on both astral and midzone microtubules rapidly bind and unbind. mEg5 punctae on midzone microtubules moved transiently both toward and away from …