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Biocompatibility Of Electrospun Polymer Nanofibers (Epns) With Nih 3t3 Cell Line And Interaction Of Quercetin With Different Flavonoids: Induction Of Phase Ii Enzymes In Vitro Cancer Cell Lines, Brenda S. Benavides
Theses and Dissertations - UTB/UTPA
Part I: In this study Electrospun nanofibers, Elasthane™ 55D, Elasthane™ 75D, Biospan®, Hydrothane™ and Lycra® were evaluated for their biocompatibility to be artificial tissue substitutes using NIH-3T3 fibroblast cells. A colorimetric based MTT dye reduction assay demonstrated that EPNs did not stop cell growth in vitro. Additionally, fluorescence confocal microscope images verified that cell adhesion occurred in the nanofibers, and cell growth on the EPNs over two weeks period was confirmed by DNA quantification.
Part II: This section of the thesis is focused on evaluation of chemopreventive properties of various phytochemicals by induction of Phase II enzymes. The interaction of …