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Nanoscale Studies Of Proteins And Thin Films Using Scanning Probe Microscopy, Zachary Lane Highland
Nanoscale Studies Of Proteins And Thin Films Using Scanning Probe Microscopy, Zachary Lane Highland
LSU Doctoral Dissertations
Nanostructures of organosilanes, thin metal films, and protein nanopatterns were prepared and analyzed with atomic force microscopy (AFM). Organosilanes with designed functional groups were used to selectively pattern green fluorescent protein at the nanoscale using protocols developed with particle lithography. Mesospheres are deposited onto a substrate to produce a surface mask. Organosilanes are deposited to form a matrix film surrounding nanopores for depositing proteins. The nanopatterns were characterized using AFM, after steps of particle lithography for directly visualizing surface changes. Studies with AFM also provide a compelling tool for teaching undergraduates to introduce concepts of nanoscience. An undergraduate laboratory was …