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Two-Dimensional And High-Throughput Electrophoretic Separation Of Proteins Using Polymeric Microchips, Hamed Shadpour
Two-Dimensional And High-Throughput Electrophoretic Separation Of Proteins Using Polymeric Microchips, Hamed Shadpour
LSU Doctoral Dissertations
A major task in proteomics is to identify proteins from a biological sample using two-dimensional (2-D) separation prior to mass spectrometry of peptides generated via proteolytic digestion of the proteins. For 2-D separations, microfluidic devices are superior to bench top and capillary-based systems since they potentially provide higher separation efficiencies due to the minimal dead volumes produced during peak transfer between the two separation dimensions. In addition, fast separations can be envisioned because the column lengths are typically shorter in microfluidic platforms without scarifying peak capacity. High-throughput capabilities are extremely desirable for many types of bio-analytical analyses, such as understanding …
Integrating Micro-Scale Separations To Matrix Assisted Laser Desorption And Ioniation Time Of Flight Mass Spectrometry (Maldi-Tof-Ms) For Protein Analysis, Harrison K. Musyimi
Integrating Micro-Scale Separations To Matrix Assisted Laser Desorption And Ioniation Time Of Flight Mass Spectrometry (Maldi-Tof-Ms) For Protein Analysis, Harrison K. Musyimi
LSU Doctoral Dissertations
This dissertation describes the integration of micro-scale separations to matrix assisted laser desorption and ionization time-of-flight mass spectrometry (MALDI TOF MS) for protein analysis. MALDI MS provides unsurpassed accurate mass measurements of intact bio-molecules, for example peptides and proteins, which in turn generate high molecular specificity enabling the identity, function and structure of these molecules to be characterized. However, in order to realize the full potential of MS in proteomic studies, integrated sample processing on automated and high throughput platforms is required to address the complexity, diversity and the dynamic range of proteomic analysis. The work described here contributes towards …