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Myoplasmic Resting Ca2+ Regulation By Ryanodine Receptors Is Under The Control Of A Novel Ca2+-Binding Region Of The Receptor, Yanyi Chen, Shenghui Xue, Juan Zou, Jose Lopez, Jenny J. Yang, Claudio Perez
Myoplasmic Resting Ca2+ Regulation By Ryanodine Receptors Is Under The Control Of A Novel Ca2+-Binding Region Of The Receptor, Yanyi Chen, Shenghui Xue, Juan Zou, Jose Lopez, Jenny J. Yang, Claudio Perez
Chemistry Faculty Publications
Passive SR (sarcoplasmic reticulum) Ca2+ leak through the RyR (ryanodine receptor) plays a critical role in the mechanisms that regulate [Ca2+ ]rest (intracellular resting myoplasmic free Ca2+ concentration) in muscle. This process appears to be isoform-specific as expression of either RyR1 or RyR3 confers on myotubes different [Ca2+ ]rest. Using chimaeric RyR3–RyR1 receptors expressed in dyspedic myotubes, we show that isoformdependent regulation of [Ca2+ ]rest is primarily defined by a small region of the receptor encompassing amino acids 3770– 4007 of RyR1 (amino acids 3620–3859 of RyR3) named as the CLR (Ca2+ leak regulatory) region. [Ca2+ ]rest regulation by the …