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2-selenouridine triphosphate; RNA transcription; RNA structure and function; hammerhead ribozyme; RNA crystallography
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2-Selenouridine Triphosphate Synthesis And Se-Rna Transcription, Huiyan Sun, Sibo Jiang, Julianne Caton-Williams, Hehua Liu, Zhen Huang
2-Selenouridine Triphosphate Synthesis And Se-Rna Transcription, Huiyan Sun, Sibo Jiang, Julianne Caton-Williams, Hehua Liu, Zhen Huang
Chemistry Faculty Publications
2-Selenouridine (SeU) is one of the naturally occurring modifications of Se-tRNAs (SeU-RNA) at the wobble position of the anticodon loop. Its role in the RNA-RNA interaction, especially during the mRNA decoding, is elusive. To assist the research exploration, herein we report the enzymatic synthesis of the SeU-RNA via 2-selenouridine triphosphate (SeUTP) synthesis and RNA transcription. Moreover, we have demonstrated that the synthesized SeUTP is stable and recognizable by T7 RNA polymerase. Under the optimized conditions, the transcription yield of SeU-RNA can reach up to 85% of the corresponding native RNA. Furthermore, the transcribed SeU-hammerhead ribozyme has the similar activity as …