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A Calorimetric Study Of Metal Binding In Carboxypeptidase, Carbonic Anhydrase And Concanavalin A, Gary E. Hatch
A Calorimetric Study Of Metal Binding In Carboxypeptidase, Carbonic Anhydrase And Concanavalin A, Gary E. Hatch
Theses and Dissertations
The purpose of this study was to perfect a new calorimetric method for identifying and counting ionizable chemical groups on proteins. To do this, calorimetric and electrometric titrations were performed (using HCl and NaOH as titrant) on native carboxypeptidase, carbonic anhydrase and concanavalin A plus their apo and organic ligand bound derivatives. Readily identifiable endpoints were seen in the calorimetric titrations and general agreement was found between ΔH values obtained in this study and literature ΔH values of model compounds undergoing the same reaction. Imidazole groups were those most easily counted. Many assumptions had to be made, however, in concluding …