Open Access. Powered by Scholars. Published by Universities.®
Articles 1 - 2 of 2
Full-Text Articles in Entire DC Network
Evaluation Of Silica Hydride Materials For The Lc–Ms Analysis Of Cathinones And Benzylpiperazines, Seiichiro Watanabe
Evaluation Of Silica Hydride Materials For The Lc–Ms Analysis Of Cathinones And Benzylpiperazines, Seiichiro Watanabe
Seiichiro Watanabe
A new LCMS approach is tested for the analysis of controlled substances using bath salts (cathinones) as a model system. Since the structure of cathinones have a polar functionality (the amine group) as well as an aromatic component, separation on the LC column could be achieved in the reversed-phase(RP) or aqueous normal phase (ANP) modes. Since silica hydride columns can function in both modes they have considerable potential for the analysis of substances that have both polar and nonpolar functionalities. Four different silica hydride-based columns (C18, phenyl, cholesterol and diol) are tested in either the RP or ANP and RP …
The Separation And Analysis Of Symmetric And Asymmetric Dimethylarginine And Other Hydrophilic Isobaric Compounds Using Aqueous Normal Phase Chromatography, Seiichiro Watanabe
The Separation And Analysis Of Symmetric And Asymmetric Dimethylarginine And Other Hydrophilic Isobaric Compounds Using Aqueous Normal Phase Chromatography, Seiichiro Watanabe
Seiichiro Watanabe
Two biologically important compounds with clinical relevance, asymmetric dimethylarginine and symmetric dimethylarginine, are analyzed using aqueous normal phase chromatography on silica hydride-based columns. Two different stationary phases were tested, a commercially available Diamond Hydride™ and a 2-acrylamido-2-methylpropane sulfonic acid experimental column. Two types of analytical protocols were investigated: analysis of the compounds when separation was achieved and analysis of the compounds with partial chromatographic separation. Urine samples from tuberculosis patients were tested for levels of asymmetric and symmetric dimethylarginine. The mass spectrometric technique of in-source fragmentation that can provide data similar to a tandem mass analyzer was evaluated as a …