Digital Commons Network^™

Nuclec Acds Encoding Pseudomonas Hop Proteins And Use Thereof, Alan Collmer, James R. Alfano, Xiaoyan Tang, C. Robin Buell, Gregory B. Martin

Department of Plant Pathology: Faculty Publications

The present invention relates to isolated nucleic acid mol ecules encoding a type III—secreted bacterial protein capable of modifying a cell death pathway in a plant cell. One aspect of the present invention involves an isolated nucleic acid molecule having a nucleotide sequence that encodes the HopPtol)2 protein of Pseudomonas syringae pv. syringae DC 3000. Expression vectors, host cells, and transgenic plants which include the DNA molecules of the present invention are also disclosed. The nucleic acid mol ecules of the present invention can be used to impart disease resistance to a plant and to make a plant hypersusceptible to …

Whole-Genome Expression Profiling Defines The Hrpl Regulon Of Pseudomonas Syringae Pv. Tomato Dc3000, Allows De Novo Reconstruction Of The Hrp Cis Element, And Identifies Novel Coregulated Genes, Adriana O. Ferreira, Christopher R. Myers, Jeffrey S. Gordon, Gregory B. Martin, Monica Vencato, Alan Collmer, Misty D. Wehling, James R. Alfano, Gabriel Moreno-Hagelsieb, Warren F. Lamboy, Geneviene Declerck, David J. Schneider, Samuel W. Cartinhour

Department of Plant Pathology: Faculty Publications

Pseudomonas syringae pv. tomato DC3000 is a model pathogen of tomato and Arabidopsis that uses a hypersensitive response and pathogenicity (Hrp) type III secretion system (T3SS) to deliver virulence effector proteins into host cells. Expression of the Hrp system and many effector genes is activated by the HrpL alternative sigma factor. Here, an open reading frame-specific whole-genome microarray was constructed for DC3000 and used to comprehensively identify genes that are differentially expressed in wild-type and ΔhrpL strains. Among the genes whose differential regulation was statistically significant, 119 were upregulated and 76 were downregulated in the wild-type compared with the ΔhrpL …

Nebline, Nov./Dec. 2006

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

Extension Offers Guardianship Trainings Mandated By the Nebraska Supreme Court
Guardianships Happen for Variety of Reasons
Pruning Ornamental Plants
Spark Up the Fireplace with Color
Test Nitrogen Levels this Fall, Begin Planning for Spring Fertilization
Free Soybean Cyst Nematode Sample Kits Available
Farming No-Till Increases for Major Nebraska Crops, Better for Environment, Fuel Saved
Computerized Farm Financial Recordkeeping Workshops
Preparing a Thanksgiving Feast Does Not Have to be Difficult!
Monte Cristo Sandwich Recipe
$tretch Your Food Dollar with Healthy Snacks
Time-Saving Kitchen Tools for Holiday Gifts
President’s Notes — Alice’s Analysis
Household Hints: Tips for a Warm, Cozy and Efficient …

Microbial Content Of Abattoir Wastewater And Its Contaminated Soil In Lagos, Nigeria, A. O. Adesemoye, B. O. Opere, S. C. O. Makinde

Department of Plant Pathology: Faculty Publications

Microbial content of wastewater in two abattoirs and the impact on microbial population of receiving soil was studied in Agege and Ojo Local Government Areas in Lagos State, Nigeria. Wastewater samples were collected from each of the abattoirs over three months period and examined for microbial content. Soil samples contaminated with the wastewaters were also collected and analyzed for microbial content as compared to soil without wastewater contamination in the neighborhood (control). Some physico-chemical parameters of the samples such as total dissolved solid, chemical oxygen demand, etc., were examined. The wastewater samples from both abattoirs were highly contaminated; Agege abattoir …

Center For Grassland Studies Newsletter, Fall 2006, Volume 12, No. 4

Center for Grassland Studies: Newsletters

Chemical Characteristics of Aging Golf Greens by Roch Gaussoin, Department of Agronomy and Horticulture, UNL

Can Ecophysiological Characteristics Explain the Success of Woody Species in the Sandhills of Nebraska? by Kathleen Eggemeyer, Tala Awada, F. Edwin Harvey, David Wedin, Xinhua Zhou and Sue Ellen Pegg, School of Natural Resources, UNL

Comparison of a Long Yearling System to Calf-fed Performance and Economics by Will Griffin and Terry Klopfenstein, Department of Animal Science, UNL

A Tale of Two States by Jana Beckman, Kansas Center for Sustainable Agriculture and Alternative Crops, K-State Research and Extension

2006 Nebraska Grazing Conference Best Yet!

Nebline, October 2006

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

Seven Steps to a Healthier Habitat in the Home
Reduce Grain Depth to Save Time/Energy When Drying Grain
Don’t Feed Fresh Silage too Soon
Beware of Wood Heating Dangers
Spider Bite or Staph Infection? Diagnosis is Important
Tips to Prevent Recurrent Head Lice Infestations
Keep Out Unwanted Pests
Household Hazardous Waste Collection
Healthy Food in a Hurry!
Awesome Banana Walnut Shake Recipe
“Cook It Quick!” Dishes Out Advice
Drying and Roasting Pumpkin Seeds
The A, B, C’s for a Healthy School
Nutrition Education Program Partners with Salvation Army’s Summer Day Camp
President’s Notes — Alice’s Analysis
Household Hints: Lower Your …

Inducible Yeast System For Viral Rna Recombination Reveals Requirement For An Rna Replication Signal On Both Parental Rnas, Hernan Garcia-Ruiz, Paul Ahlquist

Department of Plant Pathology: Faculty Publications

To facilitate RNA recombination studies, we tested whether Saccharomyces cerevisiae, which supports brome mosaic virus (BMV) replication, also supports BMV RNA recombination. Yeast strains expressing BMV RNA replication proteins 1a and 2a^pol were engineered to transiently coexpress two independently inducible, overlapping, nonreplicating derivatives of BMV genomic RNA3. B33' lacked the coat protein gene and negative-strand RNA promoter. B35' lacked the positive-strand RNA promoter and had the coat gene replaced by the selectable URA3 gene. After 12 to 72 h of induction, B33' and B35' transcription was repressed and Ura⁺ yeast cells were selected. All Ura⁺ cells …

Nebline, September 2006

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

4-H Partners With Lincoln After-School Programs
After-School Youth Participate in County Fair 4-H Activities
Free E-mail Horticulture Newsletter
Storing Vegetables and Fruit
Too Late to Spray Bagworms
Deep Watering in Fall Can Prevent Tree Death
A Clean Garden This Fall Means A Disease-Free Garden Next Spring
Take Precautions To Avoid Serious Injury When Operating ATVs
Stocking Up on MyPyramid- Style After-School Snacks
Fruit Muesli Recipe
$tretch Your Food Dollars with Beans
President’s Notes — Alice’s Analysis
Household Hints: Stain Removal Tips
Fall Maintenance
FCE News & Events
Starting the “New Year” Out Right
Chemical Control of Eastern Redcedar
Eastern Nebraska …

Nebline, August 2006

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

Kids Ag Discovery Zone at County Fair is Interactive Experience
Fun Facts About Farm Animals
Predicting the Last Irrigation for Corn, Milo and Soybeans
Effect of Increasing Energy Prices on Irrigation Costs
Fall is the Best Time to Stop Henbit
Pesticide Container Recycling Program
Add Mulches to Landscapes to Protect Plants from Temperature Changes
Water Trees During Drought
Woody Plants Under Stress
Penstemon, a Great Acreage Flower
Don’t Use Black Walnut Shavings for Animal Bedding
Are You Winning the “Fruit & Vegetable Bowl Game?”
Summertime Steak Salad Recipe
Top Ten Reasons to Eat At Home
President’s Notes — Alice’s Analysis …

Sheep Updates 2006 - Part 3, Karen Venning, Andrew Thompson, Mike Hyder, Sue-Ellen Shaw, Kelly Hill, Ron Mctaggart, Gus Rose, Carolyn Kabore, Mandy Curnow, P. R. Kenyon, S. T. Morris, D. M. West, N. R. Perkins, G. L. Pinchbeck, Ralph Behrendt

Sheep Updates

This session covers six papers from different authors:

GRAZING

1. Making better use of clover, Karen Venning and Andrew Thompson, Department of Primary Industries, Victoria

2. Grazing systems demonstration to optimise pasture utilisation and stocking rate, Mike Hyder, Sue-Ellen Shaw, Kelly Hill and Ron McTaggart, Department of Agriculture and Food Western Australia.

3. Know your audience to increase their rate of practice change - Lifetime Wool as an example, Gus Rose, Department of Agriculture and Food Western Australia, Carolyn Kabore, Kazresearch

REPRODUCTION

4. Lifetime Wool - Ewe Management Guidlines, Mandy Curnow, Department of Agriculture and Food Western Australia

5. …

Sheep Updates 2006 - Part 4, K. G. Geenty, A. A. Swan, A. J. Smith, J. L. Smith, Chris Oldham, R. G. Woodgate, R. A. Love, E. Dobbe, H. M. Hoult, J. Pearson, S. Hill, A. Van Burgel, R. B. Besier, R. Warburton, L. Mathwin, D. Rogers, E. Crossley, Allan Herbert, P. Nichols, Tony Albertsen, Darryl Mcclements, Graeme Martin, Penny Hawken, Caroline Vinoles, Beth Paganoni, Dominique Blanche

Sheep Updates

This session covers seven papers from different authors:

MANAGEMENT

1. Wool and meat traits in Merino flocks in different regions, K.G. Geenty, A.A. Swan, A.J. Smith, J.L. Smith, Sheep CRC and CSIRO Livestock Industries, Armidale

2. Fat score or Condition score? - It all depends on what you want to do! Chris Oldham, Department of Agriculture and Food Western Australia

3. Sheep worm control - the latest for Western Australia, RG Woodgate, RA Love, E Dobbe, HM Hoult, J Pearson, S Hill, A van Burgel and RB Besier, Department of Agriculture and Food Western Australia

PASTURES

4. Rethinking pasture production …

Center For Grassland Studies Newsletter, Summer 2006, Volume 12, No. 3

Center for Grassland Studies: Newsletters

Summary Analysis of Grazing Yearling Response to Distillers Grains by Terry Klopfenstein, Department of Animal Science, UNL

Grassland Ecology and Management: A New Name for the Range Major by Walter Schacht, Department of Agronomy and Horticulture, UNL

Befriending Birds: Why Should Landowners Care about Integrating Wildlife Conservation with Livestock Production? The Reasons Are Plenty! by Kindra Gordon, Grazing Lands Conservation Initiative

Another Successful Nebraska Youth Range Camp by Daryl Cisney and Mary Reece, Nebraska Section of the Society for Range Management

Landowner, Foundation, City and Federal Government Preserve Native Prairie for Grazing and Education by Steve Chick, USDA Natural Resources …

Nebline, July 2006

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

Regularly Test Private Well Drinking Water for Safety
Approved Water Testing Laboratories
Nebraska Forest Service Expert Discovers Pine Wilt Treatment
Easier Gardening
Odorous House Ant
Chiggers Not a Health Concern, But Can Make Outdoor Activities Uncomfortable
Ants in the Lawn
Bats Under Porches, Patios
Prepare to Plant Alfalfa in August
Pesticide Container Recycling Program
Apply Manure Before Seeding Alfalfa
UNL Researcher Needs Alfalfa Fields With Pocket Gophers
Planting Vegetables for Fall
Methods of Drying Flowers
Measuring Distance
Caring for Animals When Gone
Spider Mites Common Problem on Trees, Other Plants
$tretch Your Food Dollar with Fruits and Vegetables
Red, White …

Nebline, June 2006

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

Bio-Fuels Can Help Bridge Energy Gap: Nebraska in Ideal Position to be Supplier of Biofuels
When to Harvest Bromegrass Hay
Sample Your Hay to Get Accurate Nutrient Analyses
Moving Round Hay Bales Can be Dangerous
Pumping Water for Ponds
Controlling Pests with Home Remedies
What Does Work: Boric Acid and Borates
More Home Remedies Debunked
Protect Stored Winter Clothing from Insect Damage
Getting Past the Nutrition Headlines
Bone Appetit Banana Smoothie Recipe
Walk Nebraska!
President’s Notes — Alice’s Analysis
Household Hints: Storing Summer Swimsuits
FCE News & Events
Summer Energy Saving Tips
Are You Ready for Sun’s Rays?
How White …

Center For Grassland Studies Newsletter, Spring 2006, Volume 12, No. 2

Center for Grassland Studies: Newsletters

Evaluation of Two Grasses for Silvopasture Systems in the Great Plains by Michael E.L. Perry, Tala Awada, Walter Schacht, and Sue Ellen Pegg Department of Agronomy and Horticulture and School of Natural Resources, UNL

Research for the Birds: Sandhills Is the Target of Grassland Bird and Grazing Investigation by Larkin Powell, School of Natural Resources, UNL

UNL Professional Golf Management Program Scores a First

Program Set for 2006 Nebraska Grazing Conference

The Nebraska Range Shortcourse

Invasive Species Is Theme of Prairie Conference

Third National Conference on Grazing Lands

Nebline, May 2006

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

Bed Bug CSI: Investigating Bed Bug Infestations in Lincoln
2006 Perennial Plant of the Year
Cannas in the Garden
Ornamental Grasses for the Landscape
Can People Treat for Bed Bugs Themselves?
Everything Homeowners Need to Know About Termite Control Workshop, May 18
Controlling Ticks
Spring Cleaning? Take Advantage of Household Hazardous Waste Collections
Building a Bat House
Fertilizing Grass Pastures and Hay Lands
What is an Acre?
Butterfly Gardens
Family Skills and Desires
Control of Perennial Weedy Grasses
Maintaining Your Birch Tree
$tretch Your Food Dollar by $pending $mart
Saucy Pasta & Veggie Frittata Recipe
Calories Expended in Common Physical …

Nebline, April 2006

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

Visioning Process Can Help Communities Shape Their Future
Bennet is First in Nebraska to do Visioning Process
Easter Egg-Handling Tips
Lemon Dill Egg Salad Recipe
Have You Tried Kohlrabi
Food Bank Needs Volunteers, Donations
President’s Notes — Alice’s Analysis
Household Hints: Benefits of Disinfecting Household Surfaces
FCE News & Events
Poison Prevention
Tease-Proof Your Child
Acreage Insight Clinic: Horse Nutrition & Management, April 13
Effect of Increasing Energy Prices on Farming Costs
Oats for Extra Pasture, Hay and Silage
Consider Burning Shelled Corn as an Alternative Heating Fuel
Tornado – Will You be Ready?
Financial Resources and Skills
Protect Woody …

Crop Updates 2006 - Katanning, David Stephens, Michael Meuleners, Meredith Fairbanks, Thomas Schulz, T. J. Flowers, T. D. Colmer, Narelle Simpson, Ron Mctaggart, Wal Anderson, Lionel Martin, Dave Allen, Brenda Shackley, Raj Malik, Kelly Winfield, Rodger Beermier, Shahahan Miyan, Abul Hashem, Shahab Pathan, Vikki Osten, Alex Douglas, Garren Knell, Steve Curtin, Wade Longmuir, Henry Brockman, Justin Hardy, Arjen Ryder, John Paul Collins, Jessica Johns, John Pepall, Allan Herbert

Crop Updates

This session covers sixteen papers from different authors

1. 2006 SEASONAL OUTLOOK, David Stephens and Michael Meuleners, Department of Agriculture

2. Review of climate model summaries reported in the Department of Agriculture’s growing season outlooks, Meredith Fairbanks, Department of Agriculture

3. Farmers commodity outlook 2006, Thomas Schulz, Department of Agriculture

4. Why is salinity such a difficult problem for plant breeders? T J Flowers, TD Colmer, University of Western Australia

5. Matching nitrogen supply to wheat demand in 2005, Narelle Simpson, Ron McTaggart, Wal Anderson, Lionel Martin and Dave Allen, Department of Agriculture

6. Wheat varieties in 2006, Brenda Shackley, …

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Nebline, March 2006

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

Nebraska 4-H Summer Camps Provide Ultimate Camping Experience for Youth
All-America Selection Vegetables for 2006
Shamrocks for St. Patrick’s Day
Hints for Starting Transplants
Red Flour Beetles
ewf needs volunteers
Household Hazardous Waste Collections for 2006
Don’t Get Trapped in a Smelly Situation
Pocket Gophers in Your Alfalfa? UNL Wildlife Researcher Needs Your Help!
Collection of Unwanted Pesticides, March 16
Diesel vs. Propane Irrigation Systems
Commercial Pesticide Applicator Training
Worried About the High Cost of Fertilizer?
Pollination Requirements for Tree and Small Fruits
Upcoming Acreage Insights Clinics
Type of Farm Enterprise and Crop(s) Produced
$tretch Your Food Dollar with Canned …

Bacillus Species Nrrl B-30212 For Reducing Fusarium Head Blight In Cereals, David A. Schisler, Naseem L. Khan, Michael J. Boehm

Department of Plant Pathology: Faculty Publications

Four yeasts (NRRL Y-30213, NRRL Y-30214, NRRL Y-30215, and NRRL Y-30216) and 1 bacterium (NRRL B-30212) have been identified as being superior antagonists capable of suppressing Fusarium head blight (head scab) in cereals, particularly in wheat and barley. Fusarium head blight is primarily caused by the fungus Gibberella zeae (anamorph=Fusarium graminearum).

Crop Updates 2006 - Lupins And Pulses, Peter White, Mark Seymour, Amelia Mclarty, Wayne Parker, Ian Pritchard, Bob French, Rodger Beermier, Bevan Buirchell, Mark Sweetingham, Jon Clements, Geoff Thomas, Roger Jones, Sofia Sipsas, John Quealy, Leigh Smith, Gordon Francis, Huaan Yang, Jeffrey Boersma, Margaret Pallotta, Chengdao Li, Krishnapillai Sivasithamparam, M. Nelson, H. Phan, S. Ellwood, P, Moolhuijzen, M. Bellgard, J. Hane, A. Williams, J. Fos-Nyarko, B. Wolko, M. Książkiewicz, M. Cakir, M. Jones, M. Scobie, C. O'Lone, S. J. Barker, R. Oliver, W. Cowling, Laurie Maiolo, Martin Harries, Jo Walker, Murray Blyth, Stephen Cosh, Dirranie Kirby, Kedar Adhikari, Patrizia Guantini, Katie Bell, Ken Adcock, Ciara Beard, Anne Smith, Tim Maling, Art Diggle, Debbie Thackray, Kadambot Siddique, Ping Si, Huaan Yang, Vijay Jayasena, Florence Kartawinata, Ranil Coorey, Ken Dods, Kerry Regan, Tanveer Khan, Jenny Garlinge, Rod Hunter, Pam Burges, Eric Armstrong, Glen Riethmuller, Greg Shea, Moin Salam, Jean Galloway, Pip Payne, Bill Macleod, Brenda Coutts, Pooran Gaur, Heather Clarke, Stuart Morgan, Alan Harris, Ted Knights, Kristy Hobson, Chris Matthews, Colin Hanbury, Tim Pope, Mike Baker, Leanne Young, Syed Muhammad Nasar-Abbas, Julie Plummer, D. Harris, Phill Chambers, Michael Materne, Dennis Van Gool, Huyen Phan, Simon Ellwood, J. Hane, Angela Williams, R. Ford, S. Thomas, R. Oliver, Rory Coffey, Chris Poole, Theo Pfaff, Judith Lichtenzveig, Lars Kamphuis, Nola D'Souza, Emma Groves, Karam Singh

Crop Updates

This session covers sixty six papers from different authors:

2005 LUPIN AND PULSE INDUSTRY HIGHLIGHTS

1. Lupin Peter White, Department of Agriculture

2. Pulses Mark Seymour, Department of Agriculture

3. Monthly rainfall at experimental sites in 2005

4. Acknowledgements Amelia McLarty EDITOR

5. Contributors

6. Background Peter White, Department of Agriculture

2005 REGIONAL ROUNDUP

7. Northern agricultural region Wayne Parker, Department of Agriculture

8. Central agricultural region Ian Pritchard and Bob French, Department of Agriculture

9. Great southern and lakes Rodger Beermier, Department of Agriculture

10. South east region Mark Seymour, Department of …

Nebline, February 2006

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

Making Nutrition Education a Hands-On Experience for Youth
PUDDING CUP Recipe
PEANUT BUTTER BITES Recipe
YUMMY BUG Recipe
YOGURT PARFAIT Recipe
Private Pesticide Applicator Certification
Dormant Spray for Alfalfa Weeds
Commercial Pesticide Applicator Training
Crop Protection Clinic, Jan. 23
Commercial Pesticide Applicator Training
Boost Profits by at Least $20/ Acre Workshop, Feb. 15
Chemigation Certification Training
Fertilizing Crop Land with Biosolids Workshop, March 9
Dealing with Winter Flies
Beginning Beekeeping 2-Day Workshop
Be My Valentine? Consider these mating curiosities from the insect world...
Amazing Facts... About Honey and Honey bees
Household Hints: Rise and Shine with Clean Bed Linens
President’s …

Bioinformatics-Enabled Identification Of The Hrpl Regulon And Type Iii Secretion System Effector Proteins Of Pseudomonas Syringae Pv. Phaseolicola 1448a, Monica Vencato, Fang Tian, James R. Alfano, C. Robin Buell, Samuel Cartinhour, Genevieve A. Declerck, David S. Guttman, John Stavrinides, Vinita Joardar, Magdalen Lindeberg, Philip A. Bronstein, John W. Mansfield, Christopher R. Myers, Alan Collmer, David J. Schneider

Department of Plant Pathology: Faculty Publications

The ability of Pseudomonas syringae pv. phaseolicola to cause halo blight of bean is dependent on its ability to translocate effector proteins into host cells via the hypersensitive response and pathogenicity (Hrp) type III secretion system (T3SS). To identify genes encoding type III effectors and other potential virulence factors that are regulated by the HrpL alternative sigma factor, we used a hidden Markov model, weight matrix model, and type III targeting-associated patterns to search the genome of P. syringae pv. phaseolicola 1448A, which recently was sequenced to completion. We identified 44 high-probability putative Hrp promoters upstream of genes encoding the …

Regulation Of Hyphal Morphogenesis And The Dna Damage Response By The Aspergillus Nidulans Atm Homolog Atma, Iran Malavazi, Camile P. Semighini, Marcia Regina Von Zeska Kress, Steven D. Harris, Gustavo H. Goldman

Department of Plant Pathology: Faculty Publications

Ataxia telangiectasia (A-T) is an inherited disorder characterized by progressive loss of motor function and susceptibility to cancer. The most prominent clinical feature observed in A-T patients is the degeneration of Purkinje motor neurons. Numerous studies have emphasized the role of the affected gene product, ATM, in the regulation of the DNA damage response. However, in Purkinje cells, the bulk of ATM localizes to the cytoplasm and may play a role in vesicle trafficking. The nature of this function, and its involvement in the pathology underlying A-T, remain unknown. Here we characterize the homolog of ATM (AtmA) in the filamentous …

First Report Of Leaf Spot Of Smooth Bromegrass Caused By Pithomyces Chartarum In Nebraska, C. Eken, C. C. Jochum, G. Y. Yuen

Department of Plant Pathology: Faculty Publications

Smooth bromegrass (Bromus inermis Leyss.) is the most common perennial grass species cultivated for forage in North America. During late fall of 2004, smooth bromegrass plants in Lincoln, NE were observed to have brown lesions on leaf midveins that were several centimeters long. Symptomatic leaves were surface disinfested for 1 min in 2% NaOCl and incubated at 25°C on potato dextrose agar (PDA) and water agar. The fungus, Pithomyces chartarum (Berk. & Curt) Ellis, was isolated consistently and identified on the basis of morphological characteristics (1). Colonies were effused and black on PDA. Conidiophores measured 3.5 to 8 …

Fungicide Sensitivity Of Sclerotinia Homoeocarpa From Golf Courses In Ohio, Young-Ki Jo, Amy L. Niver, Joseph W. Rimelspach, Michael J. Boehm

Department of Plant Pathology: Faculty Publications

Managing dollar spot, the most common and chronic disease on intensively cultivated turfgrass, relies on the judicious use of fungicides. The heavy use of fungicides has led to the development of isolates of Sclerotinia homoeocarpa insensitive to several classes of fungicides, including benzimidazoles, demethylation-inhibitors, and dicarboximides. In vitro fungicide sensitivity assays using single discriminatory concentrations of thiophanate-methyl, propiconazole, and iprodione were developed in this study for evaluating field efficacy of these fungicides and the prevalence of fungicide insensitivity within S. homoeocarpa isolated from golf courses throughout Ohio. Discriminatory concentrations for these fungicides were determined to be: thiophanate-methyl = 1,000 μg …

Center For Grassland Studies Newsletters, Winter 2006, Volume 12, No. 1

Center for Grassland Studies: Newsletters

Nitrogen Sequestration and Groundwater Quality Charles Shapiro and Bill Kranz, Haskell Agricultural Laboratory, UNL

Maximize pre-emergence success in turf by Roch Gaussoin, Department of Agronomy and Horticulture, UNL Losing Grasslands and Natural Areas to Development by Richard Sutton, Department of Agronomy and Horticulture, UNL

New Prairie Grasses to Fatten Beef Cattle

Nike Golf Camp in Lincoln This July

Avenacin Production In Creeping Bentgrass (Agrostis Stolonifera) And Its Influence On The Host Range Of Gaeumannomyces Graminis, S. L. Thomas, P. Bonello, P. E. Lipps, Michael J. Boehm

Department of Plant Pathology: Faculty Publications

Avenacinase activity has been shown to be a key factor determining the host range of Gaeumannomyces graminis on oats (Avena sativa). G. graminis var. avenae produces avenacinase, which detoxifies the oat root saponin avenacin, enabling it to infect oats. G. graminis var. tritici does not produce avenacinase and is unable to infect oats. G. graminis var. avenae is also reported to incite take-all patch on creeping bentgrass (Agrostis stolonifera). It is unknown whether creeping bentgrass produces avenacin and if the avenacin-avenacinase interaction influences G. graminis pathogenicity on creeping bentgrass. The root extracts of six creeping bentgrass …

Carbon-To-Nitrogen Ratio And Carbon Loading Of Production Media Influence Freeze-Drying Survival And Biocontrol Efficacy Of Cryptococcus Nodaensis Oh 182.9, Shouan Zhang, David A. Schisler, Michael J. Boehm, Patricia J. Slininger

Department of Plant Pathology: Faculty Publications

Fusarium head blight (FHB), caused by Gibberella zeae, is a devastating disease of wheat worldwide. Cryptococcus nodaensis OH 182.9 is an effective biocontrol agent for this disease. Development of a dried product of OH 182.9 would have potential advantages of ease of handling, favorable economics, and acceptance by end users. Isolate OH 182.9 was grown for 48 and 72 h in semi-defined complete liquid (SDCL) medium with carbon-to-nitrogen (C/N) ratios of 6.5:1, 9:1, 11:1, 15:1, and 30:1, and in SDCL C/N 30:1 media with varied carbon loadings of 7, 14, 21, and 28 g/liter. Total biomass production and cell …

First Report Of Sudden Death Syndrome Of Soybean Caused By Fusarium Solani F. Sp. Glycines In Nebraska, A. D. Ziems, L. J. Giesler, G. Y. Yuen

Department of Plant Pathology: Faculty Publications

During August of 2004, soybean (Glycine max (L.) Merr.) plants exhibiting symptoms typical of sudden death syndrome (SDS) caused by Fusarium solani (Mart.) Sacc. f. sp. glycines (= Fusarium virguliforme Akoi, O'Donnell, Homma, & Lattanzi) (1) were observed in Nemaha and Pierce counties in eastern Nebraska. Leaf symptoms ranged from small chlorotic spots to prominent interveinal necrosis on plants at R5-R6 growth stages. Taproots of symptomatic plants were plated on potato dextrose agar (PDA) amended with hymexazol, ampicillin, and rifampicin (HAR). Resulting fungal isolates grew slowly and developed masses of blue macroconidia, characteristic of F. solani f. sp. glycines …