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An Online Lab Manual For Neurophysiology, Richard F. Olivo
An Online Lab Manual For Neurophysiology, Richard F. Olivo
Neuroscience: Faculty Publications
A combination of student interest, a grant for computers, and a suggestion from a colleague led me to convert the printed lab manual for my neurophysiology course into an online version. The web-based version incorporates videos of procedures, color photos, diagrams, links to articles, and other supplementary materials. This paper discusses designing for the web, retaining compatibility with print, layering information, making videos in digital format, and sharing self-published resources. The labs themselves (appendix) are traditional “wet” experiments with crayfish that have been used in many neurobiology courses.
Filopodial Calcium Transients Regulate Growth Cone Motility And Guidance Through Local Activation Of Calpain, Estuardo Robles, Anna Huttenlocher, Timothy M. Gomez
Filopodial Calcium Transients Regulate Growth Cone Motility And Guidance Through Local Activation Of Calpain, Estuardo Robles, Anna Huttenlocher, Timothy M. Gomez
Neuroscience: Faculty Publications
Spontaneous intracellular calcium ([Ca2+]i) transients in growth cone filopodia reduce filopodial motility, slow neurite outgrowth, and promote turning when generated asymmetrically; however, the downstream effectors of these Ca2+-dependent behaviors are unknown. We report that Ca2+ transients in filopodia activate the intracellular protease calpain, which slows neurite outgrowth and promotes repulsive growth cone turning upon local activation. Active calpain alters the balance between tyrosine kinase and phosphatase activities in filopodia, resulting in a net decrease in tyrosine phosphorylation, which mediates both filopodial stabilization and reduced lamellipodial protrusion. Our findings indicate that locally generated Ca2+ signals repel axon outgrowth through calpain-dependent regulation …