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Uncovering A Mystery Of The Isoflavonoid Metabolon: Identification And Characterization Of The Arogenate Dehydratase Gene Family In Soybean, Kelsey Pannunzio Feb 2019

Uncovering A Mystery Of The Isoflavonoid Metabolon: Identification And Characterization Of The Arogenate Dehydratase Gene Family In Soybean, Kelsey Pannunzio

Electronic Thesis and Dissertation Repository

Soybean (Glycine max) is a vastly important, multi-billion dollar global commodity; but this crop’s yields are under threat from the pathogen Phytophthora sojae, which causes extensive stem and root rot in soybean crops. Isoflavonoids, a metabolite class unique to legumes, are a promising research target to combat P. sojae. Isoflavonoids are released as phytoalexins in response to stress, and also facilitate interactions with nitrogen-fixing rhizobial bacteria through nodule formation. An isoflavonoid biosynthesis metabolon was discovered in soybean through co-immunoprecipitation, anchored to the endoplasmic reticulum by isoflavone synthase (IFS) and Cinnamate 4-hydroxylase, two cytochrome P450 enzymes. One of the …


Planting The Chalcone Reductase Family Tree: Identification And Characterization Of Chalcone Reductase Genes In Soybean, Caroline Julia Sepiol Aug 2015

Planting The Chalcone Reductase Family Tree: Identification And Characterization Of Chalcone Reductase Genes In Soybean, Caroline Julia Sepiol

Electronic Thesis and Dissertation Repository

Soybean (Glycine max [L.] Merr) is an important crop grown in Canada, generating $2.4 billion in sales. Though this number may be promising, soybean farmers lose about $50 million worth of yield annually due to root and stem rot disease caused by Phytophthora sojae. Many strategies have been developed to combat the infection; however, these methods are prohibitively expensive. A ‘cost effective’ approach to this problem is to select a trait naturally found in soybean that can increase resistance. One such trait is the increased production of root glyceollins. One of the key enzymes exclusively involved in glyceollin …