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- 4-way junction; Dna tri component probe; Molecular beacon probe; Secondary structured dna (1)
- Adrenergic hormones; Cardiac conduction; Cardiac rhythm; Cardiac rhythmicity; Heart conduction; Heart rhythm; Heart rhythmicity; Retinoic acid; Stress hormones (1)
- Affinity chromatography; Ms 818; Sds page (1)
- Androgen receptor; Bicalutamide; Casodex; Microrna; Mirna; Prostate cancer (1)
- Anthrax; Chloroplast; Diabetes; Pharmaceutical; Transformation; Transgenic; Vaccine (1)
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- Antibacterial (1)
- Antimicrobial resistance; Cerium oxide nanoparticles; Minimum inhibitory concentration; Nanoparticles; Nanotechnology (1)
- Biofuel (1)
- Biomass degrading enzymes (1)
- Chemotherapy; Dna; Homologous recombination; Nonhomologous end joining; Poison; Repair; Topoisomerase (1)
- Copper Biocide. (1)
- Copper loaded silica (1)
- Core-Shell nanoparticle (1)
- Crystallization (1)
- Crystallography (1)
- Genetically modified plants (1)
- Immunoglobulin Fab Fragments (1)
- Kinase; Life cycle; Malaria; Pfpk5; Plasmodium (1)
- Mannanase (1)
- Nasal mucosa (1)
- Peptides (1)
- Phylogeny (1)
- Protein engineering (1)
- RNA (1)
- Shake flask expression (1)
- Sol-Gel (1)
- Staphylococcus (1)
- Staphylococcus aureus infections (1)
Articles 1 - 18 of 18
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Stimulator Of Neurotropic Effects Determining The Mechanism Of Action Of The Ms-818 Compound Through Protein Identification By Affinity Chromatography And Sds-Page, Charlene Seraphina Dass
Stimulator Of Neurotropic Effects Determining The Mechanism Of Action Of The Ms-818 Compound Through Protein Identification By Affinity Chromatography And Sds-Page, Charlene Seraphina Dass
HIM 1990-2015
The MS-818 compound is used in the proliferation process of neuronal cells and many biological activities that accompany this process such as astrocyte differentiation, inhibition of neuronal apoptosis, and fraction repairs. We do know the effects of this compound, but the mechanism of action remained uncertain until now. To determine the pathway of this compound, NT2 cells were cultured and lysed to isolate the proteins. Affinity Chromatography was performed in order to immobilize the MS-818 compound to a Hi-Trap NHS column. The NT2 protein sample was injected through the column and eluted with a MS-818 concentrated, high salt content elution …
Identification Of Physiological Substrates Of Plasmodium Falciparum Pfpk5, A Cdk-Like Kinase, Catherine Sullenberger
Identification Of Physiological Substrates Of Plasmodium Falciparum Pfpk5, A Cdk-Like Kinase, Catherine Sullenberger
HIM 1990-2015
Malaria is one of the most devastating infectious diseases causing 1-3 million fatalities a year. The majority of these cases occur amongst children in developing countries. Malarial strains in these areas are exhibiting increasing resistance to canonical treatments proving the importance of new drug targets for anti-malarials. Identification of new drug targets is dependent upon a better understanding of the molecular biology of the parasitic agent of malaria, Plasmodium. The regulation of Plasmodium's complex life cycle is still not well understood. Elucidation of signaling pathways involved in Plasmodium cell cycle regulation will provide insights into how the parasite thrives in …
Microrna Regulation Of Prostate Cancer Desensitization To Androgen Receptor Antagonist Drugs During Androgen Deprivation Therapy, Robert A. Lorch
Microrna Regulation Of Prostate Cancer Desensitization To Androgen Receptor Antagonist Drugs During Androgen Deprivation Therapy, Robert A. Lorch
HIM 1990-2015
The current standard treatment of prostate cancer by androgen deprivation therapy involves using drugs such as bicalutamide (Casodex) to antagonistically block androgen receptors that are normally present within prostate cells. Usually, the therapy is successful in the short run at limiting the growth of prostate cancer. However, in virtually all cases tumors begin to grow aggressively again after several months of treatment and new therapies must be started. The mechanism by which these prostate cells transform from androgen sensitive to androgen independent and anti-androgen resistant is unclear. In this study, we investigated the role of microRNAs, small 15 to 18 …
Cross-Talk Of Retinoic Acid And Adrenergic Hormone Signaling May Influence Development Of Cardiac Conduction And Rhythmicity In Utero, Sabikha Alam
HIM 1990-2015
Stress hormones, adrenaline and noradrenaline, have been shown to be critical for heart development. Mice lacking dopamine greek lower case letter beta]-hydroxylase (Dbh), an enzyme responsible for synthesis of these adrenergic hormones, die during mid-gestation due to cardiac failure. Prior research showed that adrenergic cells are found within the electrical conduction system of the heart, and adrenergic deficiency leads to slowed cardiac conduction during embryogenesis. Microarray analysis of wild-type (Dbh+/+) and knockout (Dbh-/-) mouse hearts revealed significant differences in expression of retinoic acid (RA) signaling genes. RA signaling has also been shown to be critical for heart development. These data …
Analysis Of The Repair Of Topoisomerase Ii Dna Damage, Eric D. Goldstein
Analysis Of The Repair Of Topoisomerase Ii Dna Damage, Eric D. Goldstein
HIM 1990-2015
A large number of anti-cancer chemotherapeutics target DNA topoisomerases. Etoposide is a specific topoisomerase II poison which causes reversible double strand DNA breaks. The focus of this project is to analyze the repair of DNA damage induced by etoposide.. Double strand DNA break repair is mediated by through either non-homologous end joining (NHEJ) or homologous recombination. NHEJ repairs through direct ligation of a double stranded break while homologous recombination utilizes a homologous template to recover the wild type sequence. A reporter cassette, RYDR-GFP, has been stably integrated into HeLa cells. This reporter contains an ultra-high affinity topoisomerase II cleavage site …
A Tripartile Biosensor For Real-Time Snss Detection In Dna Hairpin Motif, Camha Nguyen
A Tripartile Biosensor For Real-Time Snss Detection In Dna Hairpin Motif, Camha Nguyen
HIM 1990-2015
The hybridization between two complementary strands of nucleic acid is the basis for a number of applications in DNA and RNA analysis, including in vivo RNA monitoring, microarrays, SNPs detection, and so on. The short oligonucleotide probes form Watson/Crick base pairs (A-T and G-C) with the analyzed nucleic acid. Molecular beacon (MB) probe is one of the most advantageous tools for nucleic acid analysis in real-time. A traditional MB probe consists of a DNA strand folded in hairpin motif with a fluorophore attached to the 5'end and a quencher attached to the 3' end. The loop segment is complementary to …
Plant-Made Oral Vaccines Evaluation Of Capsules, James Stewart New
Plant-Made Oral Vaccines Evaluation Of Capsules, James Stewart New
HIM 1990-2015
Antigen expression through the Chloroplast Transformation Technology (CTT) produces bioencapsulated subunit-vaccines, capable of eliciting immune responses when delivered orally. Considerable challenges to effective plant-based vaccines are the normalization of dosage and preservation of accumulated antigen, which is complicated by variable high water content and protease activity. This study critically examines the efficacy of lyophilization in dehydrating plant-tissues and preserving plant-derived antigens with vaccine potential. Lyophilization was optimized through gravimetric analysis using lettuce expressing Protective Antigen (PA) of Bacillus anthracis (LS-HPAG) and the human autoantigen Proinsulin (Pins) fused to Cholera toxin subunit B (LS-CTB-Pins). Lyophilization for 48-hours was sufficient treatment to …
Cerium Oxide Nanoparticles For The Detection Of Antimicrobial Resistance, Alexander J. Noll
Cerium Oxide Nanoparticles For The Detection Of Antimicrobial Resistance, Alexander J. Noll
HIM 1990-2015
The rise of antimicrobial resistance demands the development of more rapid screening methods for the detection of antimicrobial resistance in clinical samples to both give the patient the proper treatment and expedite the treatment of patients. Cerium oxide nanoparticles may serve a useful role in diagnostics due to their ability to exist in a mixed valence state and act as either oxidizing agents or reducing agents. Considering that cerium oxide nanoparticles have been shown to shift in absorbance upon oxidation, a useful method of antimicrobial resistance detection could be based on the oxidation of cerium oxide nanoparticles. Herein, an assay …
Development Of Molecular And Cellular Imaging Tools To Evaluate Gene And Cell Based Therapeutic Strategies In Vivo, Jixiang Xia
Development Of Molecular And Cellular Imaging Tools To Evaluate Gene And Cell Based Therapeutic Strategies In Vivo, Jixiang Xia
Electronic Theses and Dissertations
Molecular imaging modalities are important tools to evaluate the efficacy of gene delivery systems and cell-based therapies. Development and application of these modalities will advance our understanding of the mechanism of transgene expression and cell fate and functions. Physical gene transfer methods hold many advantages over viral vectors among gene therapeutic strategies. Here, we evaluated the efficacy of biolistic (“gene gun”) gene targeting to tissues with non-invasive bioluminescence imaging (BLI) methods. Plasmids carrying the firefly luciferase reporter gene were transfected into mouse skin and liver using biolistics, and BLI was measured at various time points after transfer. With optimized DNA …
Novel Copper Loaded Core-Shell Silica Nanoparticles With Improved Copper Bioavailability Synthesis, Characterization And Study Of Antibacterial Properties, Pavithra Maniprasad
Novel Copper Loaded Core-Shell Silica Nanoparticles With Improved Copper Bioavailability Synthesis, Characterization And Study Of Antibacterial Properties, Pavithra Maniprasad
Electronic Theses and Dissertations
A novel core-shell silica based antimicrobial nanoparticle was synthesized. The Stöber silica shell has been engineered to accommodate copper. Synthesis of the core-shell Cu-silica nanoparticle (C-S CuSiNP) involves preparation of base-hydrolyzed Stöber silica “seed” particles first, followed by the acid-catalyzed seeded growth of the Cu-silica shell layer around the core. Scanning electron microscopy and transmission electron microscopy showed monodispersed, spherical shaped nanoparticles with smooth surface morphology. Characterization of particle size distribution in solution by the Dynamic Light Scattering (DLS) technique was fairly consistent with the electron microscopy results. Loading of Cu to nanoparticles was confirmed by the SEM-Energy Dispersive X-Ray …
Expression And Functional Evaluation Of Exendin 4 Fused To Cholera Toxin B Subunit In Tobacco Chloroplast To Treat Type 2 Diabetes, Ramya Nityanandam
Expression And Functional Evaluation Of Exendin 4 Fused To Cholera Toxin B Subunit In Tobacco Chloroplast To Treat Type 2 Diabetes, Ramya Nityanandam
Electronic Theses and Dissertations
The prevalence of type 2 diabetes has been steadily increasing around the globe. Glucagon like peptide (GLP-1), a powerful incretin increases insulin secretion in a glucose dependent manner. But GLP-1 is subjected to rapid enzymatic degradation (half-life: 2 min in circulation). The commercially available GLP-1 analog, exenatide has a longer half life with potent insulinotropic effects (about 2.4 hr) which requires cold storage and daily subcutaneous injections. In this study, exendin 4 (EX4), lizard derived GLP-1R agonist, was expressed as cholera toxin B subunit (CTB)-fusion protein in chloroplasts of tobacco to facilitate transmucosal delivery in the gut by utilizing the …
A Solid Phase Assay For Topoisomerase I Interfacial Poisons And Catalytic Inhibitors, Vidusha Cyril
A Solid Phase Assay For Topoisomerase I Interfacial Poisons And Catalytic Inhibitors, Vidusha Cyril
Electronic Theses and Dissertations
We report a mechanism based screening technique to rapidly identify eukaryotic topoisomerase I targeting agents. The method is based on genetic tagging of topoisomerase I to immobilize the enzyme on a solid surface in a microtiter well format. DNA is added to the wells and retained DNA is detected by Picogreen fluorescence. Compounds that result in an increase in Picogreen staining represent potential topoisomerase interfacial poisons while those that reduce fluorescence report catalytic inhibitors; therefore, the solid phase assay represents a „bimodal‟ readout that reveals mechanisms of action. The method has been demonstrated to work with known interfacial poisons and …
Surface Entropy Reduction To Increase The Crystallizability Of The Fab-Rna Complex, Priyadarshini Palaniandy Ravindran
Surface Entropy Reduction To Increase The Crystallizability Of The Fab-Rna Complex, Priyadarshini Palaniandy Ravindran
Electronic Theses and Dissertations
Crystallizing RNA has been an imperative facet and a challenging task in the world of RNA research. Assistive methods such as Chaperone Assisted RNA Crystallography (CARC), employing monoclonal antibody fragments (Fabs) as crystallization chaperones have enabled us to obtain RNA crystal structures by increasing the crystal contacts and providing initial phasing information. Using this technology the crystal structure of [delta]C209 P4-P6 RNA (an independent folding domain of the self-splicing Tetrahymena group I intron) complexed to Fab2 (high affinity binding Fab) has been resolved to 1.95 Å (1). Although the complexed class I ligase ribozyme has also been crystallized using CARC …
Mechanisms Of Alpha]-Synuclein-Induced Neurodegenertaion In Parkinson's Disease And Stroke, Cherine Belal
Mechanisms Of Alpha]-Synuclein-Induced Neurodegenertaion In Parkinson's Disease And Stroke, Cherine Belal
Electronic Theses and Dissertations
Parkinson’s disease (PD) is a debilitating neurodegenerative disorder affecting one million Americans. Despite its social and economic impact, the pathological cascades that lead to neuron dysfunction and degeneration in PD are poorly understood. Endoplasmic reticulum (ER) stress has been implicated as an initiator or contributing factor in neurodegenerative diseases including PD. The ER is an organelle central to protein folding and intracellular Ca2+ homeostasis. Perturbations of these functions result in ER stress and upregulation of ER stress proteins, of which some have been implicated in counteracting ER stress-induced cell death. The mechanisms that lead to ER stress and how ER …
Expression Of Trichoderma Reesei Beta]-Mannanase In Tobacco Chloroplasts And Its Utilization In Lignocellulosic Woody Biomass Hydrolysis, Pankaj M. Agrawal
Expression Of Trichoderma Reesei Beta]-Mannanase In Tobacco Chloroplasts And Its Utilization In Lignocellulosic Woody Biomass Hydrolysis, Pankaj M. Agrawal
Electronic Theses and Dissertations
Lignocellulosic ethanol offers a promising alternative to conventional fossil fuels. One among the major limitations in the lignocellulosic biomass hydrolysis is unavailability of efficient and environmentally biomass degrading technologies. Plantbased production of these enzymes on large scale offers a cost effective solution. Cellulases, hemicellulases including mannanases and other accessory enzymes are required for conversion of lignocellulosic biomass into fermentable sugars. β- mannanase catalyzes endo-hydrolysis of the mannan backbone, a major constituent of woody biomass. In this study, man1 gene encoding β-mannanase was isolated from Trichoderma reesei and expressed via the chloroplast genome. PCR and Southern hybridization analysis confirmed the site-specific …
Stable Expression Of Tuberculosis Vaccine Antigen In Lettuce Chloroplasts, Priya Saikumar Lakshmi
Stable Expression Of Tuberculosis Vaccine Antigen In Lettuce Chloroplasts, Priya Saikumar Lakshmi
Electronic Theses and Dissertations
Tuberculosis (TB) is caused by Mycobacterium tuberculosis and is one of the leading reasons of death by an infectious bacterial pathogen. The development of TB vaccines has been recognized as a major public health priority by the World Health Organization. In this study, a potential candidate antigen, ESAT-6 (6 kDa early secretory antigenic target) was fused with cholera toxin B subunit (CTB). Transplastomic lettuce plants were generated expressing these fusion proteins. Site-specific transgene integration into the chloroplast genome was confirmed by polymerase chain reaction and Southern blot analysis. In transplastomic leaves, expression levels of fusion protein (CTB-ESAT6) varied depending upon …
Synthesis, Characterization And Antibacterial Activity Of Silver Embedded Silica Nanoparticle/Nanogel Formulation, Roseline Menezes
Synthesis, Characterization And Antibacterial Activity Of Silver Embedded Silica Nanoparticle/Nanogel Formulation, Roseline Menezes
Electronic Theses and Dissertations
The antibacterial property of silver (Ag) has been known since ancient time. It is reported in the literature that silver nanoparticles (AgNPs) exhibit improved antibacterial properties in comparison to silver ions of equivalent metallic Ag concentration. Such improvement in antibacterial activities is due to the high surface area to volume ratio of AgNPs (which facilitates interaction with the bacterial cells), increased release of silver ions and direct intra-cellular uptake of AgNPs leading to localized release of Ag ions. To date, over 300 consumer products containing AgNPs are available in the market and the inventory is rapidly expanding. The antibacterial efficacy …
Evolutionary Relationships Among Staphylococci And The Prevention Of Staphylococcus Aureus Nasal Colonization, Ryan Paul Lamers
Evolutionary Relationships Among Staphylococci And The Prevention Of Staphylococcus Aureus Nasal Colonization, Ryan Paul Lamers
Electronic Theses and Dissertations
Staphylococcus is a significant cause of human infection and mortality, worldwide. Currently, there are greater than 60 taxa within Staphylococcus, and nearly all are pathogenic. The collective potential for virulence among species of Staphylococcus heightens the overall clinical significance of this genus and argues for a thorough understanding of the evolutionary relationships among species. Within Staphylococcus, aureus is the most common cause of human infection, where nasal carriage of this bacterium is a known risk factor for autoinfection. The predisposition to infection by nasal carriers of S. aureus, and the ease with which strains are transferred between individuals, suggests that …