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Optimization Of Bacterial Luciferase For Expression In Mammalian Cells, Stacey Swiger Patterson
Optimization Of Bacterial Luciferase For Expression In Mammalian Cells, Stacey Swiger Patterson
Doctoral Dissertations
This study describes the optimization of expression of the bacterial luciferase enzyme in mammalian cells. Previous attempts to express this heterodimeric enzyme complex in mammalian cells have been met with only modest success. In this research effort, several vector formats were evaluated to fully determine the optimal format for the expression of these genes. It was determined that the bacterial luciferase enzyme produced optimal bioluminescence in mammalian cells when the genes were cloned and expressed as a bicistronic transcript fused with an internal ribosomal entry site (IRES). To optimize the enzyme expression further, a novel approach to codon optimize the …
Transgenic Approaches To Study Nodulation In The Model Legume, Lotus Japonicus, Crystal Bickley Mcalvin
Transgenic Approaches To Study Nodulation In The Model Legume, Lotus Japonicus, Crystal Bickley Mcalvin
Doctoral Dissertations
The soybean apyrase, GS52, characterized as an early nodulin, was further investigated for its possible role in nodulation. GS52 is expressed in roots and localized to the plasma membrane. In addition, it is rapidly induced upon rhizobial inoculation. Treatment of soybean roots with anti-GS52 antibodies blocked nodulation by Bradyrhizobium japonicum. Transgenic Lotus japonicus plants were generated expressing gs52 and showed enhanced nodulation and infection thread formation upon inoculation with Mesorhizobium loti that correlated with expression of the transgene. Surprisingly, expression of GS52 allowed L. japonicus plants to be infected but not nodulated by B. japonicum, the natural symbiont of soybean. …
Evaluation Of Microbial Inocula For Initiation Of Biological Life Support Systems For Wastewater Processing On Long Term And Deep Space Missions, Kimberly L. Cook
Evaluation Of Microbial Inocula For Initiation Of Biological Life Support Systems For Wastewater Processing On Long Term And Deep Space Missions, Kimberly L. Cook
Doctoral Dissertations
A fundamental goal of ecological research is to better understand the relationship between structural diversity, functional diversity and ecosystem stability. Insight into the mechanisms that regulate microbial ecosystem function should provide important new information to improve system control for ecological, agricultural and biotechnological applications that depend on microbial processes. For example, defined microbial consortia have been employed to improve plant growth through enhanced nutrient uptake, production of plant growth promoting hormones or control of pathogens. However, a lack of consistency of results in field application has limited the widespread use of microbial inoculants. A major goal in rhizosphere studies, therefore, …