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Using The Yeast Two-Hybrid System To Determine The Function Of Parkin E3 Ubiquitin Ligase, Vanessa Nguyen
Using The Yeast Two-Hybrid System To Determine The Function Of Parkin E3 Ubiquitin Ligase, Vanessa Nguyen
HIM 1990-2015
Parkin is a cytosolic E3 ubiquitin ligase that is recruited to the mitochondria during cellular stress and has been suggested to be involved in a variety of biological processes such as mitophagy. The recruitment of Parkin (PARK2) to the mitochondria is dependent upon the kinase activity and the accumulation of PINK1 on damaged mitochondria. Mutations in either PINK1 or Parkin genes disrupt this protective pathway and lead to the accumulation of damaged mitochondria. From a clinical standpoint, mutations in the PARK2 gene have been associated with the progression and onset of autosomal recessive juvenile parkinsonism. Without the presence of a …
Melatonin And Neurogenesis: A Comparative Study Of The Efficacy Of Melatonin, Its Precursors, And L-Dopa On Neural Stem Cell Metabolism In Human Adult Neurospheres, Omar Heriba
HIM 1990-2015
Human neurosphere stem cells offer promising potential for the treatment of neurodegenerative diseases. Their well characterized multi-potency of differentiating into neurons, astrocytes, and oligodendrocytes when exposed to the optimum exogenous growth factors make them an exciting area of study (38). Finding novel endogenous methods of modulating stem cell metabolism will allow for the safer treatment of various brain disorders (34). In this experiment, melatonin, N-acetylserotonin, L-tryptophan, and L-DOPA are added in three different concentrations to neurospheres suspended in HNSC/GBM media with less than optimal concentrations of exogenous epidermal growth factor (EGF) and fibroblast growth factor (FGF). The alamarBlue assay (resazurin) …
The Role Of Intestinal Sweet Taste Receptors (Strs) In The Regulation Of Glucose Absorption: Effects Of Short Term High Sucrose Diet (Hsd), Tania Hussain
HIM 1990-2015
Sweet taste receptors are primarily found in the oral cavity of the mammalian species. However, recent studies have shown that sweet taste receptors can be found in extraoral tissues such as the pancreas, intestines, and adipose tissue. Our lab has previously found that sweet taste receptors are down-regulated on the pancreas in the presence of high plasma glucose levels. In order to assess the possibility that sweet taste receptors respond to high levels of glucose by suppressing its expression, we wanted to see if they reacted similarly on the intestines. We found that intestinal sweet taste receptors are down regulated …