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Evaluating Human-Associated Escherichia Coli Marker Genes For Microbial Source Tracking Applications In Florida, Jacob K. Senkbeil
Evaluating Human-Associated Escherichia Coli Marker Genes For Microbial Source Tracking Applications In Florida, Jacob K. Senkbeil
USF Tampa Graduate Theses and Dissertations
Fecal pollution of surface water can lead to human health issues because waterborne transmission of pathogens is a common cause of gastroenteritis. Genetic markers developed for human-associated Escherichia coli (H8, H12, H14, and H24) are promising tools for microbial source tracking (MST) of sewage in environmental waterbodies but are untested in the US. H gene marker performance characteristics (specificity, sensitivity, and prevalence) were assessed, with the goal of developing a quantitative PCR assay for the markers with the best performance. Individual E. coli isolates from reference samples were tested to determine end-point PCR assay performance. Three hundred E. coli strains …
Mechanistic Modeling Of Photocatalytic Water Disinfection, Omatoyo Kofi Dalrymple
Mechanistic Modeling Of Photocatalytic Water Disinfection, Omatoyo Kofi Dalrymple
USF Tampa Graduate Theses and Dissertations
The main goal of this research was to develop a mechanism-based model for photocatalytic disinfection of bacteria in water using suspended catalyst pthesiss in batch reactors. The photocatalytic disinfection process occurs as a semiconductor photocatalyst, most commonly titanium dioxide (TiO2), is irradiated with light of wavelength less than 380 nm to produce hydroxyl radicals and other highly reactive oxidants which can inactivate microorganisms. Photocatalytic disinfection involves a complex interaction of many fundamental mechanisms such as light absorption and scattering by semiconductor pthesiss, electrochemical surface reactions, and heterogeneous colloidal stability. Current models, based largely on chemical reacting systems, do …