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Premature Rho-Dependent Transcription Termination In Escherichia Coli : Link To Translation And Gene Regulation, Gabriele Baniulyte
Premature Rho-Dependent Transcription Termination In Escherichia Coli : Link To Translation And Gene Regulation, Gabriele Baniulyte
Legacy Theses & Dissertations (2009 - 2024)
Transcription termination factor Rho is an essential protein in Escherichia coli and related bacteria. The primary function of Rho is to clear unproductive RNA polymerases from the DNA template to minimize negative effects associated with uncontrolled transcription. Although most of the Rho termination events are constitutive, premature Rho-mediated termination was observed at 3% of all affected transcripts indicating active regulation of Rho activity. In this work, we investigated the regulatory mechanism behind premature Rho-dependent transcription termination in two unrelated genes: suhB and topAI. We show that in both cases transcription is terminated inside the coding gene as a consequence of …
Post-Translational Modifications And Functional Studies Of Dksa In Escherichia Coli, Andrew Charles Isidoridy
Post-Translational Modifications And Functional Studies Of Dksa In Escherichia Coli, Andrew Charles Isidoridy
Legacy Theses & Dissertations (2009 - 2024)
DksA is a bacterial gene regulator that functions synergistically with the stress alarmone ppGpp to mediate the stringent response. DksA also functions independently of ppGpp to regulate transcription of a number of genes. DksA function is dependent on its binding affinity to RNA polymerase and requires specific interactions between RNAP and catalytic amino acids located on the coiled coil tip, D74 and A76. While much of the previous work on DksA has focused on understanding the mechanisms of action and the numerous gene targets for transcriptional regulation, little is known about the mechanisms by which DksA expression and function may …
Ribavirin-Resistant St. Louis Encephalitis Virus Populations Display Phenotypic And Genotypic Characteristics Of High-Fidelity Rna Polymerase Variants, Sara B. Griesemer
Ribavirin-Resistant St. Louis Encephalitis Virus Populations Display Phenotypic And Genotypic Characteristics Of High-Fidelity Rna Polymerase Variants, Sara B. Griesemer
Legacy Theses & Dissertations (2009 - 2024)
St. Louis encephalitis virus (SLEV) is mosquito-borne member of the family Flaviviridae, genus Flavivirus that is closely related to West Nile virus (WNV). Both viruses have potential to cause neuroinvasive disease in humans, and currently there is no vaccine or approved antiviral therapy. SLEV is maintained in nature through transmission between mosquitoes and birds, with humans acting as dead-end hosts. Despite their genetic and antigenic similarities, WNV and SLEV display different characteristics. WNV is known as a "generalist"; it is geographically widespread and can infect a wide variety of hosts, causing over 37,000 human cases in 48 U.S. states since …