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Microbiology

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South Dakota State University

Electronic Theses and Dissertations

Bacillus

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Interaction Of Streptomyces Closely Associated With Bacillus In Nitrogen-Limiting Conditions, Aminya Baniya Jan 2020

Interaction Of Streptomyces Closely Associated With Bacillus In Nitrogen-Limiting Conditions, Aminya Baniya

Electronic Theses and Dissertations

Biological Nitrogen Fixation is the process of conversion of atmospheric dinitrogen into ammonia which is performed by symbiotic as well as free-living diazotrophic bacteria. A previous project to isolate free-living nitrogen fixing bacteria from a natural prairie yielded Streptomyces which grew on nitrogen free medium. Genome sequencing of three isolates revealed the presence of a second 16S rRNA gene sequence aligning with Bacillus, however repeated streaking only yielded Streptomyces colonies. The aim of this research was to characterize the interaction of three Streptomyces isolates that grow under nitrogen-free conditions, and appeared to be associated with Bacillus. The separation of Bacillus …


Diversity Of Free – Living Nitrogen – Fixing Bacteria In Soil Of Sioux Prairie Of South Dakota, Nabilah Ali Alshibli Jan 2018

Diversity Of Free – Living Nitrogen – Fixing Bacteria In Soil Of Sioux Prairie Of South Dakota, Nabilah Ali Alshibli

Electronic Theses and Dissertations

There are only two natural ways by which molecular nitrogen can be available to support life, either by free-living or by symbiotic nitrogen fixing bacteria. The best studied diazotrophs form symbiotic associations with plants, primarily legumes and certain tree species, but little is known about how non-leguminous plants such as grasses obtain nitrogen in their environment. Natural prairies have few legumes, thus have less symbiotic nitrogen fixer interaction. This indicates presence of free-living nitrogen (FLN) fixation activity towards the balance of the N cycle. The objective of this study was to characterize the culturable diversity of free-living diazotrophs in native …