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Antibacterial And Antibiofilm Efficacy Of Pineapple Hump (Ananas Comosus) On Porphyromonas Gingivalis In Vitro, Abdul Gani Soulissa, Billy Lombardo, Armelia Sari Widyarman

Journal of Dentistry Indonesia

Background: Periodontal disease is one of the most prevalent oral health problems in Indonesia that affects supporting tissues of the teeth. Porphyromonas gingivalis plays an important role in the pathogenesis of periodontal disease. Alternative therapy with natural plant extracts, including pineapple (Ananas comosus) hump extract may inhibit the growth of bacteria that cause periodontal disease. Objective: To determine the effect of pineapple hump extract on bacterial growth and adhesion of Porphyromonas gingivalis biofilms. Method: The bacterial inhibition test was performed by the agar well diffusion method, and biofilm density measurements were made using the biofilm assay method. Results: Pineapple hump …

Functionalizing Nanoparticles With Cafa Protein To Target Bar Peptide For Oral Delivery Applications., Hetal Desai

Electronic Theses and Dissertations

Background: Porphyromonas gingivalis adherence to Streptococcus gordonii may be important for P. gingivalis colonization in the oral cavity. Nanoparticles encapsulating synthetic peptide BAR (BAR-NPs) inhibit P. gingivalis adherence more potently than free BAR. However, BAR-NPs would exhibit low retention in an open flow environment. Hypothesis: Targeting BAR-NPs to the streptococcal surface using CafA protein will enhance their efficacy. Methods: CafA-modified NPs encapsulating BAR were synthesized using double emulsion approach. Surface binding and retention, and release kinetics of BAR from CafA-modified NPs was assessed. Functional inhibition assays were performed using dual a species biofilm. Results: CafA-modified NPs demonstrated specificity of adhesion, …

Possible Pathologic Effect Of Hmgb1 On P. Gingivalis Induced Inflammatory Response By Macrophages, Michelle Marie Torres Santos

Student Theses, Dissertations and Capstones

BACKGROUND: Periodontitis is an inflammatory disease caused by poly-microbial infection that leads to destruction of connective tissue and alveolar bone. It is well documented that bacteria-derived virulent factors that can act on Toll-like receptors (TLRs), represented by Lipopolysaccharides (LPS), are engaged in the initiation of inflammatory responses. However, while LPS is also produced by the bacteria colonized in the healthy periodontal tissue, inflammation is not induced by LPS in those periodontal healthy subjects, suggesting the requirement of additional factor to upregulate the LPS-mediated inflammatory response in periodontal tissue. Recent studies revealed that novel class of endogenous proinflammatory mediator, high mobility …

Disruption Of Immune Homeostasis In Human Dendritic Cells Via Regulation Of Autophagy And Apoptosis By, Mohamed M Meghil, Omnia K Tawfik, Mahmoud Elashiry, Mythilypriya Rajendran, Roger M Arce, David J Fulton, Patricia V Schoenlein, Christopher W Cutler

Student and Faculty Publications

As fundamental processes of immune homeostasis, autophagy, and apoptosis must be maintained to mitigate risk of chronic inflammation and autoimmune diseases. Periodontitis is a chronic inflammatory disease characterized by oral microbial dysbiosis, and dysregulation of dendritic cell (DC) and T cell responses. The aim of this study was to elucidate the underlying mechanisms by which the oral microbe

Synthesis And Characterization Of Targeted Bar Encapsulated Polylactic-Co-Glycolic Acid Nanoparticles To Inhibit Porphyromonas Gingivalis Biofilm Formation., Ranjith Radha Krishnan

Electronic Theses and Dissertations

Periodontal disease is one of the most prevalent infectious diseases worldwide. Between 30-50% of the global adult population suffers from periodontal disease. Some form of periodontitis is present in 46% of American adults, corresponding to annual expenditures in excess of 14 billion dollars for treatment and prevention. Current treatments for periodontal diseases involve mechanical removal of plaque, correction of risk factors, gingival surgery and/or antibiotic therapy. To our knowledge there is no effective therapeutic approach that aims to limit pathogen colonization of the oral biofilm or re-colonization after treatment. Interaction of the pathogen Porphyromonas gingivalis with oral streptococci is critical …

Influences Of Porphyromonas Gingivalis On The Viability Of Esophageal Cancer Cells., Muddasir Mohammed

Electronic Theses and Dissertations

A recent study demonstrated an association between the Porphyromonas gingivalis (Pg)infection and the progression of ESCC (Gao et al., 2016). However, how Pg infection affects the nature of ESCC remains unknown. We examined the effects of Pg on the proliferation and chemotherapy drug-induced apoptosis of ESCC cells and elucidated the signaling molecules involved in these processes. Method: Apoptosis and cell proliferation were tested using Flow cytometry and Tetrazolium salt based proliferation assays respectively. Western Blot was utilized to detect the protein expression. CDK2 and Cyclin E were knocked down in the ESCC cells through transfection. Unpaired T-test and …

Development Of Blended Polymeric Fibers To Alter The Release Of Peptide Targeting Porphyromonas Gingivalis., Sonali Suresh Sapare

Electronic Theses and Dissertations

Periodontitis is a chronic inflammatory disease that infects the tissues of the periodontium. It is estimated that 47.2% or 67.2 million American adults suffer from mild, moderate and severe periodontitis. Globally, 30-50% of the adult population is afflicted with periodontal disease, making it one of the most prevalent infectious diseases in the world. Therapeutics targeting P. gingivalis may be effective to alter periodontitis progression. However, the current treatment modalities that target critical pathogens to maintain host-biofilm homeostasis are limited, urging the development of specifically targeted therapeutics to limit P. gingivalis recolonization of the oral cavity after periodontal treatment and healing. …

Biochemical Analysis Of Putative Rna-Binding Proteins In Porphyromonas Gingivalis, Jacob Mirpanah

Theses and Dissertations

BIOCHEMICAL ANALYSIS OF PUTATIVE RNA-BINDING PROTEINS IN PORPHYROMONAS GINGIVALIS

By Jacob Mirpanah

Bachelor of Arts in Biology, University of Virginia, 2015

A thesis submitted in partial fulfillment of the requirements for the degree of Master of Science in Physiology and Biophysics at Virginia Commonwealth University

Virginia Commonwealth University, 2018

Major Director: Janina P. Lewis, Ph.D., Philips Institute for Oral Health Research

RNA-Binding Proteins (RBPs) play important regulatory roles in countless cellular processes. Often via the induction of a structural change in RNA’s secondary structure, RBPs are known to modulate protein expression through post-transcriptional regulation. In Escherichia coli, such RBPs have …

Ccl3 And Cxcl12 Production In Vitro By Dental Pulp Fibroblasts From Permanent And Deciduous Teeth Stimulated By Porphyromonas Gingivalis Lps, Carla Renata Sipert, Ana Carolina Morandini, Karin Cristina Da Silva Modena, Thiago José Dionísio, Maria Aparecida Andrade Moreira Machado, Sandra Helena Penha Oliveira, Ana Paula Campanelli, Carlos Ferreira Santos

Ana Carolina Morandini

OBJECTIVE: The aim of this study was to compare the production of the chemokines CCL3 and CXCL12 by cultured dental pulp fibroblasts from permanent (PDPF) and deciduous (DDPF) teeth under stimulation by Porphyromonas gingivalis LPS (PgLPS). MATERIAL AND METHODS: Primary culture of fibroblasts from permanent (n=3) and deciduous (n=2) teeth were established using an explant technique. After the fourth passage, fibroblasts were stimulated by increasing concentrations of PgLPS (0-10 µg/mL) at 1, 6 and 24 h. The cells were tested for viability through MTT assay, and production of the chemokines CCL3 and CXCL12 was determined through ELISA. Comparisons among samples …

Identification And Characterization Of Microrna Differentially Expressed In Macrophages Exposed To Porphyromonas Gingivalis Infection, Olivier Huck, J Al-Hashemi, L Poidevin, O Poch, J Davideau, H Tenenbaum, Salomon Amar

NYMC Faculty Publications

MicroRNAs (miRNAs) are short, noncoding RNAs involved in the regulation of several processes associated with inflammatory diseases and infection. Bacterial infection modulates miRNA expression to subvert any innate immune response. In this study we analyzed, using microarray analysis, the bacterial modulation of miRNAs in bone marrow-derived macrophages (BMMs) in which activity was induced by infection with Porphyromonas gingivalis The expression of several miRNAs was modulated 3 h postinfection (at a multiplicity of infection of 25). A bioinformatic analysis was performed to further identify pathways related to the innate immune host response under the influence of selected miRNAs. To assess the …

Nitrosative Stress Sensing In Porphyromonas Gingivalis: Structure And Function Of The Heme Binding Transcriptional Regulator Hcpr, Benjamin R. Belvin

Theses and Dissertations

Porphyromonas gingivalis, a Gram negative anaerobe implicated in the progression of periodontal disease, is capable of surviving and causing infection despite high levels of reactive nitrogen species found in the oral cavity due to its efficient nitrosative stress response. HcpR is an important sensor-regulator that plays a vital step in the initiation of the nitrosative stress response in many Gram negative anaerobic bacteria. We employ a combination of X-ray crystallography, SAXS, resonance Raman spectroscopy, UV-Vis spectroscopy, and molecular biology techniques to better understand this key regulator. Knockout of the hcpR gene in W83 P. gingivalis results in the inability of …

Mechanism Of Interaction Of Peptide Modified Nanoparticles With Porphyromonas Gingivalis., Ankita Jain

Electronic Theses and Dissertations

Studies suggest that P. gingivalis functions as a keystone pathogen and interacts with primary colonizers in the supragingival biofilm such as S. gordonii. This interaction contributes to the initial colonization of the oral cavity by P. gingivalis and thus represents a potential target for therapeutic intervention. We have identified a peptide (BAR) derived from the streptococcal SspB protein that functions to inhibit P. gingivalis adherence to S. gordonii. In addition, we showed that nanoparticles (NPs) functionalized with BAR inhibit this interaction more potently than free soluble peptide, possibly by promoting interaction with P. gingivalis at higher valency than …

Characterization Of Pg1604 (Porz) As An Essential Component Of Type 9 Secretion System In Porphyromonas Gingivalis., Apurva Tadimari Prabhakar

Electronic Theses and Dissertations

Background: The numerous virulence factors of Porphyromonas gingivalis are essential for the organism to invade host tissues while escaping immune responses [1, 2]. Some of these factors, like major proteases referred to as gingipains, are secreted by a unique secretion system called PerioGate or Type IX Secretion System (T9SS). T9SS consists of a number of membrane and periplasmic proteins, which provide transportation only for proteins that possess a conserved C-terminal domain (CTD). The CTD functions as a specific recognition signal for the T9SS. PG1604 of P. gingivalis W83 is a CTD-containing protein, which has been shown in our initial studies …

Tobacco-Gene Activity Profile In Porphyromonas Gingivalis, Filifactor Alocis And Treponema Denticola., Neelima Chowdary Cherukumalli

Electronic Theses and Dissertations

Smoking is an established risk factor for periodontitis. Prior studies have shown that cigarette smoke extract (CSE) can induce profound phenotypic changes in Porphyromonas gingivalis and alters the virulence of this important periodontal pathogen. We hypothesized that CSE might also alter gene expression in established periodontal pathogens, Porphyromonas gingivalis and Treponema denticola, as well as in the emerging pathogen, Filifactor alocis. Oral bacteria were grown in CSE-conditioned medium (1000 ng/ml nicotine equivalents) or in unconditioned control medium. Total RNA was extracted and CSE-regulated genes were identified by comparison of the mRNA profiles of CSE with control cultures using RNA-Seq …

The Study Of The Regulon Of Oxyr In Escherichia Coli And Porphyromonas Gingivalis, Christopher K. Pham

Theses and Dissertations

The facultative anaerobe, Escherichia coli and the obligate anaerobe, Porphyromonas gingivalis are two bacteria that reside in our body. Although they reside in separate environments, they are both subject to hydrogen peroxide stress and have mechanisms to regulate the stress. OxyR is the primary transcriptional regulator/sensor of oxidative stress response caused by hydrogen peroxide. OxyR in P. gingivalis is not well-characterized compared to OxyR in E. coli. We sought to characterize and compare the two forms of OxyR in order to gain a better understanding of the protein. We determined the oligomeric state of both proteins: primarily a tetramer for …

Synthesis And Functional Evaluation Of Peptide Modified Poly (Lactic-Co-Glycolic Acid) Nanoparticles To Inhibit Porphyromonas Gingivalis Biofilm Formation., Paridhi Kalia

Electronic Theses and Dissertations

Periodontal disease is an oral inflammatory disorder that afflicts roughly 46% of the adults in the U.S. Currently, treatment of periodontal disease involves the removal of plaque from the gingival pocket (with possible antibiotic treatment) and if necessary, gingival surgery. To our knowledge, no therapeutic approach exists that promotes host-biofilm homeostasis by limiting pathogen recolonization of the oral cavity after prophylaxis or treatment. The interaction of the pathogen Porphyromonas gingivalis with commensal streptococci is critical for initiation of periodontitis and represents a target for limiting P. gingivalis colonization of the oral cavity. Previous studies showed that a synthetic peptide …

Neutrophils Alter Epithelial Response To Porphyromonas Gingivalis In A Gingival Crevice Model., Jessica Lynn Bondy-Carey 1987-

Electronic Theses and Dissertations

A gingival crevice model (epithelial cell-Porphyromonas gingivalis-neutrophil) was established and used to profile gingipain, matrix metalloproteinase (MMP), MMP mediators [neutrophil gelatinase-associated lipocalin (NGAL) and tissue inhibitor of metalloproteinases 1 (TIMP-1)] and cytokine networks. Smoking is the primary environmental risk factor for periodontitis. Therefore, the influence of cigarette smoke extract (CSE) was also monitored in the same model. Porphyromonas gingivalis alone induced low levels of interleukin-1b and interleukin-8 from epithelial cells, but high levels of both cytokines were produced on the addition of neutrophils. Exposure to CSE (100 and 1000 ng mL1 nicotine equivalency) significantly compromised P. gingivalis-induced cytokine secretion (both …

Palatal Mucosa Derived Fibroblasts Present An Adaptive Behavior Regarding Cytokine Secretion When Grafted Onto The Gingival Margin, Fabíola Pontes Azevedo, Ana Carolina Morandini, Carla Renata Sipert, Thiago José Dionísio, Carlos Ferreira Santos, Carla Andreotti Damante, Maria Lúcia Rubo De Rezende, Adriana Campos Passanezi Sant'ana, Sebastião Luiz Aguiar Greghi

All Dugoni School of Dentistry Faculty Articles

BACKGROUND: Considering that grafted gingival tissue might have to be adapted to the receptor area and that fibroblasts have the ability to respond to bacterial stimuli through the release of various cytokines, this study investigated whether fibroblasts from the palatal mucosa behave differently when grafted onto the gingival margin regarding cytokine secretion.

METHODS: Biopsies from the palatal mucosa were collected at the time of free gingival graft surgery, and after four months re-collection was performed upon surgery for root coverage. Fibroblasts were isolated by the explant technique, cultured and stimulated with Porphyromonas gingivalis (Pg) and Escherichia coli (Ec) LPS for …

Characterization Of Putative Porphyromonas Gingivalis Rna-Binding Proteins, Holly Dwyer

Theses and Dissertations

Porphyromonas gingivalis (P. gingivalis) is a gram-negative, anaerobic bacterium recognized as a major player in progression of periodontal disease. P. gingivalis survives in the oral cavity while being exposed to dynamic environmental conditions such as pH, temperature, nutrient availability and host immune responses such as oxygen tension and nitrosative stress. Survival and pathogenesis of P. gingivalis in the oral cavity require mechanisms to regulate gene expression in response to the extracellular signals. Little is known about the regulatory mechanisms of P. gingivalis in the oral cavity, so it is important to investigate and characterize these regulatory mechanisms. Adaptation to environmental …

Role Of Vimf In Gingipain Maturation In Porphyromonas Gingivalis, Arun S. Muthiah

Loma Linda University Electronic Theses, Dissertations & Projects

Gingipain activity in Porphyromonas gingivalis, the major etiological agent in adult periodontitis, is post-translationally regulated by unique Vim proteins including VimF, a putative glycosyltransferase. To ascertain the VimF mediated phenotype we first inactivated the vimF gene in P. gingivalis ATCC 33277 (FLL476), a less virulent fimbriated strain. We observed that the vimF-defective mutant (FLL476) showed a phenotype similar to that of the vimF-defective mutant (FLL95) in the P. gingivalis W83 background. While hemagglutination was not detected and autoaggregationn was reduced, biofilm formation was increased in FLL476. Also, invasive capacity decreased for this mutant. Furthermore, fimbrial structures were missing in FLL476, …

Ccl3 And Cxcl12 Production In Vitro By Dental Pulp Fibroblasts From Permanent And Deciduous Teeth Stimulated By Porphyromonas Gingivalis Lps, Carla Renata Sipert, Ana Carolina Morandini, Karin Cristina Da Silva Modena, Thiago José Dionísio, Maria Aparecida Andrade Moreira Machado, Sandra Helena Penha Oliveira, Ana Paula Campanelli, Carlos Ferreira Santos

All Dugoni School of Dentistry Faculty Articles

OBJECTIVE: The aim of this study was to compare the production of the chemokines CCL3 and CXCL12 by cultured dental pulp fibroblasts from permanent (PDPF) and deciduous (DDPF) teeth under stimulation by Porphyromonas gingivalis LPS (PgLPS).

MATERIAL AND METHODS: Primary culture of fibroblasts from permanent (n=3) and deciduous (n=2) teeth were established using an explant technique. After the fourth passage, fibroblasts were stimulated by increasing concentrations of PgLPS (0-10 µg/mL) at 1, 6 and 24 h. The cells were tested for viability through MTT assay, and production of the chemokines CCL3 and CXCL12 was determined through ELISA. Comparisons among samples …

Investigating The Transcriptional Regulation By Oxyr In Porphyromonas Gingivalis., Anuya R. Paranjape

Theses and Dissertations

Periodontal diseases are bacterially induced, inflammatory diseases which are responsible for loss of alveolar bone and connective tissue supporting the teeth which results in loss of teeth. Gram negative anaerobic bacteria are highly associated with these diseases. One of them is Porphyromonas gingivalis belonging to the phylum Bacteroidetes. Infection by P. gingivalis is recurrent after physical removal of the bacteria from the oral cavity and even after antibiotic treatment as development of resistance is not rare. Hence complete understanding the biology of this bacterium is of significance. This gram negative obligate anaerobe, being aerotolerant, manages to survive inside the oral …

Oral Microbial Biofilm Stimulation Of Epithelial Cell Responses, Rebecca Peyyala, Sreenatha S. Kirakodu, Karen F. Novak, Jeffrey L. Ebersole

Center for Oral Health Research Faculty Publications

Oral bacterial biofilms trigger chronic inflammatory responses in the host that can result in the tissue destructive events of periodontitis. However, the characteristics of the capacity of specific host cell types to respond to these biofilms remain ill-defined. This report describes the use of a novel model of bacterial biofilms to stimulate oral epithelial cells and profile select cytokines and chemokines that contribute to the local inflammatory environment in the periodontium. Monoinfection biofilms were developed with Streptococcus sanguinis, Streptococcus oralis, Streptococcus gordonii, Actinomyces naeslundii, Fusobacterium nucleatum, and Porphyromonas gingivalis on rigid gas-permeable contact lenses. Biofilms, as well as planktonic cultures …

Role Of Extracytoplasmic Function Sigma Factors In Porphyromonas Gingivalis, Suhasini Yanamandra Sai

Theses and Dissertations

Porphyromonas gingivalis is a major etiological agent that is responsible for the cause and progression of periodontal diseases. The bacterium is exposed to various environmental conditions and oxidative stress conditions while it is in the oral cavity. So, P. gingivalis should have an efficient regulatory system in order to adjust and survive in the oral cavity. But little is known about the regulatory mechanisms that help the bacteria to survive in the oral cavity. So, it is essential to understand and characterize these regulatory mechanisms. The response and adaptation of P. gingivalis to environmental stress conditions occur at the level …

Presence Of Porphyromonas Gingivalis In Gingival Squamous Cell Carcinoma, J. Katz, M. Onate, Kaleb M. Pauley, I Bhattacharyya, S. Cha

Science and Mathematics Faculty Publications

Periodontal disease has been recently linked to a variety of systemic conditions such as diabetes, cardiovascular disease, preterm delivery, and oral cancer. The most common bacteria associated with periodontal disease, Porphyromonas gingivalis (P. gingivalis) has not yet been studied in the malignant gingival tissues. The objective of this study was to investigate the presence of P. gingivalis in specimens from squamous cell carcinoma patients. We have performed immunohistochemical staining to investigate the presence of P. gingivalis and Streptococcus gordonii (S. gordonii), a non invasive oral bacteria, in paraffin embedded samples of gingival squamous cell carcinoma (n=10) and …

Mmp-9/Timp-1 Imbalance Induced In Human Dendritic Cells By Porphyromonas Gingivalis, Ravi Jotwani, Sridhar V K Eswaran, Surinder Moonga, Christopher W Cutler

Student and Faculty Publications

Matrix metalloproteinase-9 (MMP-9) cleaves collagen, allowing leukocytes to traffic toward the vasculature and the lymphatics. When MMP-9 is unregulated by tissue inhibitor of metalloproteinase-1 (TIMP-1), this can lead to tissue destruction. Dendritic cells (DCs) infiltrate the oral mucosa increasingly in chronic periodontitis, characterized by infection with several pathogens including Porphyromonas gingivalis. In this study, human monocyte-derived DCs were pulsed with different doses of lipopolysaccharide of P. gingivalis 381 and of Escherichia coli type strain 25922, as well as whole live isogenic fimbriae-deficient mutant strains of P. gingivalis 381. Levels of induction of MMP-9 and TIMP-1, as well as interleukin-10 (IL-10), …

Bactericidal Effects Of Low Temperature Atmospheric Pressure Plasma On Porphyromonas Gingivalis, Arwa Mahasneh

Dental Hygiene Theses & Dissertations

Purpose: The biomedical application of low temperature atmospheric pressure plasma (LTAPP) is a collaborative interest for engineering, medical, dental and biological researchers. This laboratory study tested whether LTAPP can limit growth of Porphyromonas gingivalis, a periodontal pathogen strongly associated with periodontal disease, disease progression, and refractory periodontitis. Methods: After extensive pilot trials, the study consisted of 24 agar plate samples of P. gingivalis- 20 samples were exposed to LTAPP at 5, 7, 9 and 10 minutes and 4 control samples were exposed to helium gas only. Immediately after exposures, the samples were incubated anaerobically for 72 hour at …

Characterization Of Chemical And Biological Properties Of Purified Porphyromonas Gingivalis Free Dihydroceramides, Andrew Robert Chapokas

SoDM Masters Theses

The aim of the present investigation is to purify, characterize, and determine the chemical and biological properties of Porphyromonas gingivalis free dihydroceramides (fDHC). Effects were tested by examination of human gingival fibroblast morphologic changes in addition to their synthesis of prostaglandin E2 (PGE2) and prostaglandin F2α (PGF2&#;) produced in vitro. Free dihydroceramides as well as phosphoglycerol dihydroceramide (PGDHC), and de-esterified phosphoglycerol dihydroceramide (PGDHC-C15:0) were obtained by total lipid extraction of P. gingivalis followed by high performance liquid chromatography (HPLC). Confirmation and structural analysis was obtained by electrospray mass spectrometry (ES-/+MS) and nuclear magnetic resonance spectroscopy (NMR). A synthetic free dihydroceramide …

Efficacy Of The De Novo-Derived Antimicrobial Peptide Wlbu2 Against Oral Bacteria, Karen F. Novak, William J. Diamond, Sreenatha S. Kirakodu, Rebecca Peyyala, Kimberly W. Anderson, Ronald C. Montelaro, Timothy A. Mietzner

Center for Oral Health Research Faculty Publications

The efficacy of a novel synthetic antimicrobial peptide (WLBU2) was evaluated against three oral microorganisms (grown planktonically): Streptococcus gordonii, Fusobacterium nucleatum, and Porphyromonas gingivalis. WLBU2 killed all three species, with F. nucleatum being the most susceptible. WLBU2 also reduced the bacterial burden of S. gordonii and F. nucleatum biofilms.

การระบุเชื้อพอร์ฟีโรโมแนส จิงจิวาลิส ที่นํามาจากร่องลึกปริทันต์อักเสบชนิดแอดวานซ์ โดยวิธีพีซีอาร์, จินตกร คูวัฒนสุชาติ

Chulalongkorn University Dental Journal

วัตถุประสงค์ การศึกษาครั้งนี้ ต้องการนําวิธีพีซีอาร์ มาใช้ยืนยันการระบุเชื้อพอร์ฟีโรโมแนส จึงจิวาลิส ที่นํามาจาก ร่องลึกปริทันต์อักเสบชนิดแอดวานซ์ของผู้ป่วยจํานวน 3 ราย วัสดุและวิธีการ ทําการแยกเชื้อแบล็คพิคเม็นเต็ด แบคทีรอยดีส (3 ชนิด ประกอบด้วยเชื้อหมายเลข 1, 2 และ 3) จากเชื้อที่ได้จากร่องลึกปริทันต์ของผู้ป่วยโรคปริทันต์อักเสบชนิดแอดวานซ์ 3 ราย โดยผู้ป่วยเป็นชาวไทยที่มารับ การรักษาโรคปริทันต์จากภาควิชาปริทันตวิทยา คณะทันตแพทยศาสตร์ จุฬาลงกรณ์มหาวิทยาลัย ทําการเพาะเลี้ยง เชื้อที่แยกมาได้ (ทั้ง 3 ชนิดนี้) ลงบนจานอาหารเลี้ยงเชื้อพวกเบรนฮาร์ทอินฟิวชั่นยีสต์เอ็กแทรกบลัดชีพอาการใน ตู้อบที่ปราศจากออกซิเจนที่มีไนโตรเจนร้อยละ 80 ไฮโดรเจนร้อยละ 10 และคาร์บอนไดออกไซด์ร้อยละ 10 ที่อุณหภูมิ 37 องศาเซลเซียส เป็นเวลา 10 วัน แล้วนําเชื้อแบล็คพิคเม็นเต็ด แบคทีรอยดีส (ทั้ง 3 ชนิดที่เพาะเลี้ยง ขึ้นเรียบร้อยแล้ว) ไปทําการแยกดีเอ็นเอ สําหรับการทําพีซีอาร์ เพื่อทําการระบุชนิดของเชื้อต่อไป ผลการศึกษา ผลที่ได้จากการศึกษา ปรากฏว่า เชื้อแบล็คพิคเซ็นเต็ด แบคทีรอยดีส 3 ชนิด ที่ประกอบด้วยเชื้อ หมายเลข 1, 2 และ 3 นั้น เชื้อหมายเลข 1 กับเชื้อหมายเลข 2 ให้แถบกับไพรเมอร์พีจี (19+20) กับไพรเมอร์พีจี (21+22) ซึ่งตรงกับกลุ่มควบคุมบวกที่ให้แถบกับไพรเมอร์พีจี (19+20) กับไพรเมอร์พีจี (21+22) ส่วนเชื้อหมายเลข 3 ไม่ให้แถบกับไพรเมอร์พีจี (19+20) กับไพรเมอร์พีจี (21+22) สรุป วิธีพีซีอาร์เป็นวิธีที่เหมาะต่อการนํามาใช้ระบุเชื้อพีจีจากผู้ป่วยด้วยโรคปริทันต์อักเสบ เพราะสามารถระบุเชื้อพีจีได้รวดเร็วและแม่นยํา (ว ทันต จุฬาฯ 2549;29:161-170)