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Degradation Of 23s Rrna In Azithromycin-Treated Ribonuclease Mutants Of Escherichia Coli., Jessica A. Silvers
Degradation Of 23s Rrna In Azithromycin-Treated Ribonuclease Mutants Of Escherichia Coli., Jessica A. Silvers
Electronic Theses and Dissertations
Azithromycin, a macrolide antibiotic, specifically binds to the 50S ribosomal subunit of bacterial ribosomes and inhibits translation. Azithromycin also prevents 50S ribosomal subunit assembly by binding to a 50S ribosomal subunit precursor particle. When exposed to azithromycin, several ribonucleases in wild-type Escherichia coli cells degrade antibiotic-bound 50S precursor particles. Presumably, cells expressing one or more mutated ribonucleases will degrade the antibiotic-bound precursor less efficiently, resulting in increased sensitivity to the antibiotic. To test this, eight ribonucleaseûdeficient strains of Escherichia coli were grown in the presence or absence of azithromycin. Cell viability, growth rates, and protein synthesis rates were measured. Degradation …
Role Of Macrophage Apoptosis In Atherosclerosis., June Liu
Role Of Macrophage Apoptosis In Atherosclerosis., June Liu
Electronic Theses and Dissertations
The presence of apoptotic cells in atherosclerotic lesions has been broadly reported in the past ten years. The majority of these apoptotic cells are macrophages. However, the pathogenic role of macrophage apoptosis in the development of atherosclerosis remains to be elucidated. Elevated expression of Bax, one of the pivotal pro-apoptotic proteins of the Bcl-2 family, has been found in human atherosclerotic plaques. Activation of Bax also occurs in free cholesterol-loaded and oxysterol treated mouse macrophages. In this study, we evaluated the influence of Bax deficiency on apoptosis in macrophage-like P388D1 cells by using small interfering RNA (siRNA) to suppress Bax …
Dose-Dependent Effects Of Salmon Calcitonin On Bone Turnover In Ovariectomized Rats., Beatrice H. Owens
Dose-Dependent Effects Of Salmon Calcitonin On Bone Turnover In Ovariectomized Rats., Beatrice H. Owens
Electronic Theses and Dissertations
In the United States, osteoporosis results in about 1.5 million annual fractures, costing approximately $15 billion. Calcitonin is safe and effective in slowing osteoporotic bone loss, but its effect is transient. The current studies were designed to explore the dose-dependent effects of salmon calcitonin on bone turnover in ovariectomized rats and to determine if the decrease in therapeutic effectiveness of calcitonin demonstrated over time with higher doses is due to oversuppression of bone turnover. Doses of 5, 15, & 50 IU/kg BW/day of calcitonin were compared to placebo in 12-week-old ovariectomized and sham-ovariectomized Sprague-Dawley rats for 24 weeks. The spinal …
Access To Care: A Hospice Model Of Access To Care For The Homeless, Mary Ann Bridget Kinney
Access To Care: A Hospice Model Of Access To Care For The Homeless, Mary Ann Bridget Kinney
Theses and Graduate Projects
Access to Hospice for the terminally ill patient is a monumental problem for the disenfranchised person. According to the National Coalition for the Homeless (NCH) in Washington D.C. there is an estimate of 3.5 million people who experience homelessness in a year (2003). The homeless face many health care service gaps including the vital service of end- of- life nursing care, which allows for death with dignity. The following paper will discuss the author's experience with the homeless, the need of access to Hospice Care for the homeless, and a review of literature regarding access to Hospice for the homeless. …
Nursing Students' Self-Efficacy And Attitude: Examining The Influence Ofthe Omaha System In Nurse Managed Centers, Cherie Mooy
Nursing Students' Self-Efficacy And Attitude: Examining The Influence Ofthe Omaha System In Nurse Managed Centers, Cherie Mooy
Master's Projects
Self-efficacy, or confidence, as an outcome behavior has been identified as influencing nursing job satisfaction and retention. Clinical learning environments and teaching strategies that build and support perceived self-efficacy are critical aspects of preparing new nurses for their entry and continuing role as professional nurses in today's information-intensive data-management healthcare environment. The purpose of this pre-test post-test study is to measure, using the C-scale (Grundy, 1992), nursing students' self-efficacy to perform patient assessment in Nurse Managed Centers (NMC) after one semester of using the Omaha System documentation framework. Nursing students' attitudes of preparation for using Standardized Nursing Languages (SNL) in …
Growth Stimulation Of Pulmonary Adenocarcinoma And Their Cells Of Origin By "Chemopreventive" Agents That Increase Intracellular Camp, Hussein Abdulhadi Nasser Al-Wadei
Growth Stimulation Of Pulmonary Adenocarcinoma And Their Cells Of Origin By "Chemopreventive" Agents That Increase Intracellular Camp, Hussein Abdulhadi Nasser Al-Wadei
Doctoral Dissertations
Lung cancer is the leading cause of cancer mortality for men and women in the United States, with a high mortality rate and a five-year survival rate of less than 15%. Cancer ranks second as a cause of death for Americans after cardiovascular disease. The American Cancer Society (ACS) reported 171,900 new cases of lung cancer for 2003 (ACS, 2003). Peripheral adenocarcinoma (PAC) of the lung has increased dramatically over the last 20 years and is the leading histological type of lung cancer in smokers and nonsmokers in industrialized countries, including the United States. Among the four main histological lung …
Inhibition Of Angiogenesis Using Rnai Technology, Yunsang Lee
Inhibition Of Angiogenesis Using Rnai Technology, Yunsang Lee
Doctoral Dissertations
Ocular infection of Herpes Simplex Virus-1 (HSV-1) causes Herpetic Stromal Keratitis (HSK), which is a leading cause of infectious blindness. Although complex interactions of molecular and cellular events involve in the department of HSK, it has been known that angiogenesis is a key step for the HSK pathogenesis. Treatment of neutralizing antibody against vascular endothelial growth factor (VEGF) to inhibit VEGF activity reduced angiogenesis and HSK severity caused by infection of HSV-1 in mouse eyes. In addition, inactivation of cytokine which induces VEGF production and angiogenesis also reduced angiogenesis and HSK severity. Therefore, it has been proposed that VEGF or …
Control Of P27 Localization And Degradation By The Pi3 Kinase Akt/Pkb Pathway In Mcf-7 Breast Cancer Cells, Marissa J. Bradbury
Control Of P27 Localization And Degradation By The Pi3 Kinase Akt/Pkb Pathway In Mcf-7 Breast Cancer Cells, Marissa J. Bradbury
Doctoral Dissertations
Breast cancer is caused by a variety of environmental and genetic factors that influence cell growth and survival. Changes in the level or function of cell cycle regulatory proteins are often associated with breast cancer. Low expression of the cell cycle inhibitor, p27Kip1, is associated with aggressive breast tumors. Clinical observations have inspired studies to analyze p27 as a potential target for signal transduction pathways in cancer cells. The stability of p27 determines it’s ability to regulate the cell cycle, and changes in transcription and translation of p27 are less influential. The regulation of the cellular localization of …
Bad Is A Central Player In Cell Death And Cell Cycle Regulation In Breast Cancer Cells, Romaine Ingrid Fernando
Bad Is A Central Player In Cell Death And Cell Cycle Regulation In Breast Cancer Cells, Romaine Ingrid Fernando
Doctoral Dissertations
BAD is a Central Player in Cell Death and Cell Cycle Regulation in Breast Cancer Cells
The estrogen, 17-b estradiol (E2) can stimulate proliferation or induce differentiation or death depending on the cell context. In MCF 7 cells, an estrogen receptor (ER) positive human breast cancer cell line, E2 has been shown to stimulate cell proliferation and may have an anti-apoptotic effect. E2 rapidly activates mitogen activated protein kinases (MAPKs) in mammalian cells in an ER dependent fashion. The mechanisms underlying and the physiological significance of the signaling through MAPK remain to be determined. This signal pathway may …
The Potential Role Of Ledgf/P75 In Prostate Cancer, Tracy Ruth Daniels
The Potential Role Of Ledgf/P75 In Prostate Cancer, Tracy Ruth Daniels
Loma Linda University Electronic Theses, Dissertations & Projects
The serum autoantibody repertoire from cancer patients is currently being exploited for the identification of tumor associated antigens (TAA) and the design of TAA arrays. Such arrays would facilitate autoantibody profiling and potentially aid in the serological diagnosis and prognosis of tumors. The goal of this study was to identify and characterize prostate-specific TAA. We observed that while the general frequency and titers of autoantibodies in PCa patients were relatively similar to those in matched controls, significant differences could be detected between the two groups in the autoantibody response to the lens epithelial-derived growth factor p75 (LEDGF/p75). LEDGF/p75 is a …
Papain: A Novel Urine Adulterant., David Burrows
Papain: A Novel Urine Adulterant., David Burrows
Electronic Theses and Dissertations
The estimated number of employees in the United Stated screened annually for illicit drugs is approximately 20 million, with marijuana being the most frequently abused drug. Urine adulterants provide an opportunity for illicit drug users to obtain a false negative result on commonly used primary drug screening methods such as the Fluorescence Polarized Immunoassay (FPIA) technique. Typical chemical adulterants such as nitrites are easily detected or render the urine specimen invalid as defined in the proposed federal guidelines for specimen validity testing based on creatinine, specific gravity and pH. Papain is a cysteine protease with intrinsic ester hydrolysis capability. The …
The Effect Of Naocl, Ca(Oh)2, Mta And Mtad On Root Dentin Fracture Resistance, Sunil Ilapogu
The Effect Of Naocl, Ca(Oh)2, Mta And Mtad On Root Dentin Fracture Resistance, Sunil Ilapogu
Loma Linda University Electronic Theses, Dissertations & Projects
Various materials are used for the treatment of immature teeth that have been subjected to trauma or decay. It is not well established what effect these materials may have on the immature root dentin. A concern would be the resistance to fracture of the remaining root dentin. The purpose of this study was to compare the resistance of fracture of bovine teeth treated with sodium hypochlorite, calcium hydroxide, gray MTA and gray MTA in conjunction with MTAD after certain periods of time. One hundred and ninety five freshly extracted, intact bovine incisors were prepared using a modified Haapasalo and Orstavik …
The Effect Of Topical Fluorides On The Microleakage Of Sealants, N. Jared Nation
The Effect Of Topical Fluorides On The Microleakage Of Sealants, N. Jared Nation
Loma Linda University Electronic Theses, Dissertations & Projects
Purpose: The purpose of this study was to evaluate the possible microleakage found in two groups of sealants; a group bonded with a dentin bonding agent (DBA), and a group bonded without a DBA after exposure to a variety of topical fluorides.
Materials and Methods: Extracted, human molars were placed into 8 groups. Half of the groups contained sealants that were bonded with a DBA and the other remaining groups of sealants were bonded without a DBA. There were two control groups, one that had sealants bonded with a DBA and the other had sealants bonded without a DBA, but …
Cbct Panoramic Images Vs. Traditional Panoramic Radiographs, Sunny Young Hutchinson
Cbct Panoramic Images Vs. Traditional Panoramic Radiographs, Sunny Young Hutchinson
Loma Linda University Electronic Theses, Dissertations & Projects
The purpose of this research was to compare the accuracy of angular measurements of the panoramic image of the Newtom 9000™ Cone Beam Computed Tomography (CBCT) with the Sirona™ Orthophos panoramic unit. The study was separated into three parts: phantom study, patient study, and archform study. The phantom study used fabricated phantom typodonts to compare angular measurements between the Newtom 9000™ panoramic image and the Sirona™ panoramic radiograph. Accuracies of the measurements were then determined by comparing the values to the actual phantom typodont. No marked differences in angular measurements were recorded for the phantom typodont using either of the …
The Influence Of Performance Success, Skill Level, And Gender On The Causal Attributions Of Recreational Golfers, Kevin W. Mchenry
The Influence Of Performance Success, Skill Level, And Gender On The Causal Attributions Of Recreational Golfers, Kevin W. Mchenry
Graduate Theses
The purpose of this study was to examine the effect of performance success, skill level, and gender on the causal attributions made by recreational golfers. Skill level was determined by handicap, and performance success was the golfer’s estimation of whether the particular round of golf was successful or unsuccessful. It was hypothesized that the causal attributions that recreational golfers made would be more internal, stable, and personally controllable after a successful performance than after an unsuccessful performance. Male and female golfers (N=101), from age 19 to 73, with an average age of 39, playing recreationally completed the Causal Dimension Scale-ll …
P14arf : A P53-Independent Tumor Suppressor, Monte W. Miller
P14arf : A P53-Independent Tumor Suppressor, Monte W. Miller
Loma Linda University Electronic Theses, Dissertations & Projects
Many genetic alterations at the CDKN2A locus on human chromosome 9 have been shown to be at least partially responsible for transformation of cells to a cancerous phenotype. This locus encodes two proteins, p16 and p14, that play a pivotal role in tumor surveillance.
Breakdowns in the p14 pathway have been estimated to be present in approximately 40% of human cancers and only recently have its binding partners and effects begun to be defined. Its interaction with the p53 pathway, which is estimated to be inactivated or mutated in 50% of all cancers, makes it difficult to determine its own …
Folk Healing In The Tradition Of The Fidencista Movement, Katherine Brittain
Folk Healing In The Tradition Of The Fidencista Movement, Katherine Brittain
Theses and Dissertations - UTB/UTPA
The Fidencista Movement is a religion in process . Like in many other medico-religions whose primary ministry is healing, women hold the highest positions of authority. The shamanic channeling of the spirit of the folk saint, Niño Fidencio , plies Fidencistas' belief in the spirit world and ameliorates physical and emotional healing. Fidencistas are concentrated in the Texas/Mexico borderland region where they are facing a passing of traditionalism, the social matrix in which folk healing thrives. However, the signature of the Movement, the penitential trek up the Vía de Dolores in Espinazo, is a cohering bi-annual ritual that fortifies devotion …
Enterotoxin B Subunit Lectins As Adjuvants For Improvement Of Mucosal Vaccine, Nak-Won Choi
Enterotoxin B Subunit Lectins As Adjuvants For Improvement Of Mucosal Vaccine, Nak-Won Choi
Loma Linda University Electronic Theses, Dissertations & Projects
In comparison with whole organism vaccines, subunit vaccines may be safer for immunization but may lack sufficient immunogenicity to provide complete immunity to the pathogen. To resolve this problem, bacterial and plant enterotoxin B subunit adjuvants containing a variety of receptor-binding properties were used to enhance the immunogenicity of rotavirus subunit vaccines. Enterotoxin B subunit adjuvants were employed to enhance protection against virus infection. Pentameric cholera toxin B subunit (CTB), shiga toxin-1 B subunit (STB) and monomeric ricin toxin B subunit (RTB) molecules were genetically linked to a 90 amino acid peptide from the simian rotavirus (SA11) nonstructural protein NSP4 …
The Effect Of Rapid Maxillary Expansion On Nasal Cavity Volume And Nasal Airway Resistance, Todd Ehrler
The Effect Of Rapid Maxillary Expansion On Nasal Cavity Volume And Nasal Airway Resistance, Todd Ehrler
Loma Linda University Electronic Theses, Dissertations & Projects
This study examined the effects of Rapid Maxillary Expansion (RME). Using Cone Beam CT scanning technology and Rhinomanometry, the volume and nasal airway resistance (NAR) of the nasal cavity was measured immediately before and after RME.
27 subjects (16 female, and 11 male), ages 9-18 were enrolled in the study. After RME all subjects had a statistically significant increase in nasal cavity volume. The pre expansion group experienced a statistically significant reduction in NAR when compared to the post expansion group. Individuals with a high pre expansion NAR experienced the greatest reduction in NAR. The correlation between change in volume …
The Effects Of Nursing Back Rub On Pain And Wound Cytokines And The Relationship Between Pre-Cabg Mood And Post-Cabg Wound Cytokines, Kathlene D. Smith
The Effects Of Nursing Back Rub On Pain And Wound Cytokines And The Relationship Between Pre-Cabg Mood And Post-Cabg Wound Cytokines, Kathlene D. Smith
Doctoral Dissertations
This study, guided by psychoneuroimmunology theory, examined the effects of a 10-minute nursing back rub in 20 coronary artery bypass graft (CABG) patients on pain and four proinflammatory wound cytokines (IL-1β, IL-6, IL-8, and TNF-α). It also examined the relationships between the Profile of Mood States (POMS) and the proinflammatory cytokines. A control group (n = 8) received time with the researcher, and an experimental group (n = 12) received a slow stroke effleurage back rub. All participants initially completed the POMS and after surgery, wound fluid was collected from the mediastinal chest tube before and after the intervention. Analysis …
Identification Of Ligand-Receptor Interactions Between Saccharomyces Cerevisiae Α-Factor Pheromone Receptor (Ste2p) And Its Tridecapeptide Ligand, Çağdaş Devrim Son
Identification Of Ligand-Receptor Interactions Between Saccharomyces Cerevisiae Α-Factor Pheromone Receptor (Ste2p) And Its Tridecapeptide Ligand, Çağdaş Devrim Son
Doctoral Dissertations
G protein-coupled receptors (GPCRs) are a class of integral membrane receptor proteins that are characterized by a signature seven-transmembrane (7TM) configuration. These receptors comprise a large and diverse gene family found in fungi, plants, and the animal kingdom. Recent studies with GPCRs have begun to elucidate their importance in many physiological processes, thus various human diseases are associated with GPCR pathology. Although the overall 3D structure of these receptors carry similar features, binding of an extraordinarily diverse array of ligands trigger many different biological pathways.
The α-factor receptor (Ste2p) of Saccharomyces cerevisiae belongs to the GPCR family. Upon the α-factor …
Childhood Experiences Of Appalachian Women Who Have Experienced Intimate Partner Violence During Adulthood., Amy L. Reeves
Childhood Experiences Of Appalachian Women Who Have Experienced Intimate Partner Violence During Adulthood., Amy L. Reeves
Electronic Theses and Dissertations
Recent national surveys in the United States estimate one in five females will experience abuse by an intimate partner during her lifetime. Previous quantitative research linked childhood victimization to repeated victimization in adult relationships. This qualitative study explored the lived experiences of childhood in eight female victims of intimate partner violence who were born and reared in southern Appalachia. Interviews were analyzed using a descriptive-interpretative phenomenological method, as described by Van Manen. The three essential themes from childhood were identified as: living 'as if' an orphan; surviving in chaos; and, manifesting a devalued self. These themes were congruent with findings …
Novel Vaccine Strategies To Prevent Herpes Simplex Virus Infection, Christopher D. Pack
Novel Vaccine Strategies To Prevent Herpes Simplex Virus Infection, Christopher D. Pack
Masters Theses
Herpes simplex virus, which infects a majority of the population, remains a significant human pathogen for which no effective vaccine exists. Despite decades of intensive research, this virus has resisted numerous classical and cutting edge approaches. We can only hope that additional research into new vaccines ideas as well as the fundamentals of how the virus interacts with the immune system will someday lead to the design of an effective vaccine. The first part of this dissertation focuses on the virus and how it interacts with various immune cells in the body. HSV-1 exerts a number of influences on a …
A Closer Look At Development Of Self-Awareness And Compensatory Strategy Use In Adults With Traumatic Brain Injury, Melissa A. Byrne
A Closer Look At Development Of Self-Awareness And Compensatory Strategy Use In Adults With Traumatic Brain Injury, Melissa A. Byrne
Masters Theses
My study investigates the development of self-awareness and use of compensatory strategies in eight adults with traumatic brain injuries using the Awareness Questionnaire (AQ) and three additional questions of descriptive nature. This study also examines whether or not the severity of brain injury (mild, moderate, severe) impacts the development of self-awareness. In addition, the spouses' perceptions of client awareness are compared to the relatives' to determine existence of potential variance.
Results indicate that while there is no significant difference in self-awareness_ between the mild and moderate groups, the severe group significantly varies from both the mild and moderate groups. Also, …
The Effectiveness Of Personal Data Assistants In Improving Daily Function Of Adults With Mild Traumatic Brain Injury, Connie Lee Budiwarman
The Effectiveness Of Personal Data Assistants In Improving Daily Function Of Adults With Mild Traumatic Brain Injury, Connie Lee Budiwarman
Masters Theses
This study evaluated the effectiveness of using Personal Data Assistants to help with memory and/or organizational difficulties with adult patients who had survived mild Traumatic Brain Injury. Utilizing an AB single subject design method with a training period, one subject ultimately completed the study. The study demonstrated mixed results. Through the use of daily record sheets, the subject reported improvement in three areas of daily function but reported negative improvement in three areas of daily function. This study suggests, but does not completely support, the potential use of Personal Data Assistants with patients with Traumatic Brain Injury.
A Survey Of California's Nurse Practitioners' Regarding Practice, Knowledge And Atitudes Of The National Asthma Management Guidelines, Colleen M. Hughes
A Survey Of California's Nurse Practitioners' Regarding Practice, Knowledge And Atitudes Of The National Asthma Management Guidelines, Colleen M. Hughes
Master's Projects
Background: Pediatric asthma morbidity and mortality continues to rise in the United States despite the institution of the National Institute of Health/National Heart, Lung and Blood Institute's diagnostic and treatment guidelines. This study examines the practices, knowledge and attitudes of California's pediatric and family nurse practitioners regarding the national asthma guidelines. Method: A quantitative mail-in survey of pediatric, pulmonary, emergent, urgent care, and family nurse practitioners was utilized to gather the data regarding the care of pediatric asthma patients. The survey collected demographic data and the NP' s current asthma practices in a Likert scale format. Results: The nurse practitioners …
Fostering Clinical Judgment During Preceptorship, Susan Mcniesh
Fostering Clinical Judgment During Preceptorship, Susan Mcniesh
Master's Projects
The role transition from student nurse to new graduate nurse commonly includes a preceptor relationship. Preceptorships are often the culminating nursing education experience for senior baccalaureate nurses and are used as well for orienting new graduates as they begin their staff nurse role in the hospital setting. The preceptee is paired with an experienced nurse who is charged with supporting, teaching, and exemplifying safe and competent patient care. In many cases preceptors are ascribed the task of independently validating the preceptee's ability to exercise clinical judgment. Today's health care environment requires nurses to be proficient critical thinkers. Patients need more …
Demographics And Perceptions Of Work Environment For Registered Nurses, Pamela Brotherton Sedano
Demographics And Perceptions Of Work Environment For Registered Nurses, Pamela Brotherton Sedano
Master's Projects
Registered nurses (RNs) are the lifeblood of hospitals. Therefore, retaining skilled nurses is necessary to insure the viability of these institutions. A two-year longitudinal, non-experimental research study utilized a descriptive design to compare the perceptions of RNs who remained on their units to those who left or changed units over a two-year time period. The purpose of this study was to ascertain whether there was a statistically significant difference between these two groups. Results in several areas indicate that further evaluation is necessary by nurse managers and administration. This information could help retain RNs as well as attract qualified nurses …
A Study Of Pediatric Heart Transplant Patients In Relationship To Post Transplant Coronary Artery Disease, Mark Randall
A Study Of Pediatric Heart Transplant Patients In Relationship To Post Transplant Coronary Artery Disease, Mark Randall
Loma Linda University Electronic Theses, Dissertations & Projects
Post transplant coronary artery disease influences short and long-term survival of pediatric heart transplant recipients. Associated cardiovascular disease risk factors among non-heart transplant adult and pediatric heart populations for development of coronary artery disease have been documented. Little is known about these typical cardiovascular disease risk factors as well as other possible risk factors in the development of post transplant coronary artery disease. Objective: The purpose of this research was to examine children/adolescents (n=194), 5-18 years old, at least one-year post heart transplant. This study also investigated if there were physiological and psychological/behavioral differences between recipients with (n = 28) …
Benefits Of A Postgraduate Education: The Physician Assistants' Perspective, Jamie Cotter
Benefits Of A Postgraduate Education: The Physician Assistants' Perspective, Jamie Cotter
Theses and Graduate Projects
Background: This study was completed to better understand the use of postgraduate education in the physician assistant profession. This research project gathered information to better understand how physician assistants have utilized postgraduate education in their careers. The potential benefits of a postgraduate education were also examined from the perspective of the physician assistant. The overall goal was to determine the benefits of a PA postgraduate education. Methods: For this research project, a survey was completed by practicing physician assistants who attended the Minnesota Academy of Physician Assistants (MAPA) Conference/North Central Regional Meeting that was held on March 27-29, 2003. This …