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Evaluation Of The Effect Of Different Concentrations Of Nanocurcumin On Periodontal Fibroblastic Cell Culture Isolated From Periodontitis Patient, Thamar A. Bossiela, Naglaa S. El-Kilani, Eman M. Kandeel, Amal A. Ibrahim
Evaluation Of The Effect Of Different Concentrations Of Nanocurcumin On Periodontal Fibroblastic Cell Culture Isolated From Periodontitis Patient, Thamar A. Bossiela, Naglaa S. El-Kilani, Eman M. Kandeel, Amal A. Ibrahim
Al-Azhar Journal of Dentistry
Purpose: The goal of this study was to see how varying concentrations of nanocurcumin (NC) affected the culture of periodontal ligament fibroblastic cells (PDLFCs). Materials and Methods: Nanocurcumin was prepared at concentrations (10 µmol/mL, 3 µmol/mL, 1.5 µmol/mL, 0.7 µmol/mL and , 0.3 µmol/mL ). Periodontal ligament fibroblastic cells were extracted, characterized using Flowcytometry and cultured in Dulbecco's Modified Eagle Medium (DMEM). The healthy PDLFCs served as the positive control group, however the PDLFCs of periodontitis patients were divided into two subgroups. 1st subgroup: PDLFCs treated with NC different concentrations , counted by hemocytometer and Cell proliferation assay was done …
Evaluation Of The Effect Of Different Concentrations Of Rosehip On Gingival Fibroblastic Cell Culture, Mai H. Mohamed, Naglaa S. El Kilani, Entsar E. Badr, Omneya E . Ahmed
Evaluation Of The Effect Of Different Concentrations Of Rosehip On Gingival Fibroblastic Cell Culture, Mai H. Mohamed, Naglaa S. El Kilani, Entsar E. Badr, Omneya E . Ahmed
Al-Azhar Journal of Dentistry
Purpose: The purpose of this study was to see how varied rosehip concentrations affected gingival fibroblastic cell culture. Materials and Methods: we prepared different concentrations of Rosehip oil (RHO) and RHO with Solvent (RHO/S). Gingival fibroblast cells were isolated from gingivitis patient. Gingival fibroblast cells were cultured, characterized, and analyzed in two subgroups. 1st subgroup: gingival fibroblast cells were treated with RHO RHO\S at different concentrations (5, 10) µg/mL, counted using a hemocytometer. Trypan blue and the cell proliferation assay3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) were done at 24, 48, 72hours. The half-maximal effective concentration (EC50) of RHO and RHO/S was …